Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	317,683	(G)5→6	intergenic (‑547/+43)	ykgA ← / ← rclC	putative DNA‑binding transcriptional regulator YkgA/reactive chlorine species resistance protein C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	317,678	1	.	G	100.0%	302.7 / NA	83	intergenic (‑542/+48)	ykgA/rclC	putative DNA‑binding transcriptional regulator YkgA/reactive chlorine species resistance protein C
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (44/39);  total (44/39)

TGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAA‑‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCA  >  NC_000913/317541‑317813                                                                                                                                           |                                                                                                                                         tGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAA‑‑gg                                                                                                                                       <  2:586235/140‑1 (MQ=255)       gCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAA‑‑ggggggcc                                                                                                                                 >  2:1079644/1‑137 (MQ=255)           tAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGAc                                                                                                                              <  1:965224/140‑1 (MQ=255)             aaaaTACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGc                                                                                                                            >  2:708160/1‑140 (MQ=255)                aTACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGGGCCTGACGCCt                                                                                                                          >  1:122581/1‑140 (MQ=255)                  aCATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGaa                                                                                                                       >  2:289622/1‑140 (MQ=255)                  aCATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGaa                                                                                                                       >  2:339998/1‑140 (MQ=255)                     tgattgatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGaaaaa                                                                                                                    >  1:862066/1‑140 (MQ=255)                     tgattgatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGaaaaa                                                                                                                    >  1:830635/1‑140 (MQ=255)                      gattgatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAGGGGGGGCCTGACGCCTGaaaaa                                                                                                                    <  2:122581/140‑1 (MQ=255)                         tgatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGa                                                                                                                <  1:410369/140‑1 (MQ=255)                         tgatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGa                                                                                                                <  1:789601/140‑1 (MQ=255)                          gatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGaa                                                                                                               <  1:771962/140‑1 (MQ=255)                             atTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGaacaa                                                                                                            >  2:645801/1‑140 (MQ=255)                                aaTCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAacag                                                                                                         >  1:502831/1‑140 (MQ=255)                                 aTCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAacaga                                                                                                        <  1:645801/140‑1 (MQ=255)                                 aTCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAacaga                                                                                                        <  2:502831/140‑1 (MQ=255)                                 aTCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAacaga                                                                                                        <  1:1021594/140‑1 (MQ=255)                                 aTCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAacaga                                                                                                        <  2:830635/140‑1 (MQ=255)                                   cATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAacagaca                                                                                                      <  1:52658/140‑1 (MQ=255)                                     tGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAgt                                                                                                    >  1:790651/1‑140 (MQ=255)                                      gTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGCGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAacaga                                                                                                        <  2:1013767/135‑1 (MQ=255)                                      gTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACGACAGACAgtg                                                                                                   >  1:112650/1‑140 (MQ=255)                                      gTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAacaga                                                                                                        >  1:1013767/1‑135 (MQ=255)                                      gTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAgtg                                                                                                   >  2:697494/1‑140 (MQ=255)                                       tATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAacaga                                                                                                        >  1:435469/1‑134 (MQ=255)                                       tATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAacaga                                                                                                        <  2:435469/134‑1 (MQ=255)                                       tATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAgtgt                                                                                                  >  2:54256/1‑140 (MQ=255)                                       tATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAgtgt                                                                                                  >  1:307112/1‑140 (MQ=255)                                           ttACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGaacaa                                                                                                            <  1:349519/126‑1 (MQ=255)                                           ttACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGaacaa                                                                                                            >  2:349519/1‑126 (MQ=255)                                           ttACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCgg                                                                                              <  1:1079644/140‑1 (MQ=255)                                            tACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTTGGa                                                                                             >  2:472075/1‑140 (MQ=255)                                             aCCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGAt                                                                                            <  2:790651/140‑1 (MQ=255)                                              ccTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGAtt                                                                                           >  1:428553/1‑140 (MQ=255)                                                       acAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATAtt                                                                                  <  2:733088/140‑1 (MQ=255)                                                       acAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATAtt                                                                                  <  1:1042638/140‑1 (MQ=255)                                                        cAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTc                                                                                 >  1:934394/1‑140 (MQ=255)                                                         aaaaaCCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTG‑AAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAg                                                                               >  1:1032798/1‑140 (MQ=255)                                                                   tAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACg                                                                      <  1:339998/140‑1 (MQ=255)                                                                       aGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGa                                                                  >  2:393319/1‑140 (MQ=255)                                                                          gAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACt                                                               <  1:289622/140‑1 (MQ=255)                                                                          gAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACt                                                               <  1:488919/140‑1 (MQ=255)                                                                          gAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACt                                                               <  1:472075/140‑1 (MQ=255)                                                                          gAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACt                                                               <  2:934394/140‑1 (MQ=255)                                                                          gAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACt                                                               <  1:966712/140‑1 (MQ=255)                                                                          gAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACt                                                               <  1:348855/140‑1 (MQ=255)                                                                               atatTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGAtt                                                          <  1:393319/140‑1 (MQ=255)                                                                                   tGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGaa                                                                 >  2:942551/1‑129 (MQ=255)                                                                                   tGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGaa                                                                 <  1:942551/129‑1 (MQ=255)                                                                                     aTCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTAc                                                    >  1:830747/1‑140 (MQ=255)                                                                                     aTCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTAc                                                    >  2:5949/1‑140 (MQ=255)                                                                                       cTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTg                                                  >  1:107300/1‑140 (MQ=255)                                                                                       cTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTg                                                  >  1:435944/1‑140 (MQ=255)                                                                                         tCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAGCAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCg                                                <  1:708160/140‑1 (MQ=255)                                                                                          cACTCTGTCATATATCCGGTTATATGCCGTCAGGCTTCATCATCAGACA‑‑GGGGGGCGGACGCCGGAAAGAGTGAACAACAGACAGTGTTGGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCgtt                                              >  2:324750/1‑140 (MQ=255)                                                                                                 tCATATCTCCGGTAATATTGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTaa                                        >  2:761582/1‑140 (MQ=255)                                                                                                           ggTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGTCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCcg                              >  1:1067872/1‑140 (MQ=255)                                                                                                           ggTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGGCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCg                                                >  2:746264/1‑122 (MQ=255)                                                                                                           ggTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCg                                                <  1:746264/122‑1 (MQ=255)                                                                                                           ggTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCg                                                <  2:4188/122‑1 (MQ=255)                                                                                                           ggTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCg                                                >  1:4188/1‑122 (MQ=255)                                                                                                           ggTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCcg                              >  2:991230/1‑140 (MQ=255)                                                                                                           ggTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCcg                              >  1:1032008/1‑140 (MQ=255)                                                                                                           ggTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCcg                              >  1:233731/1‑140 (MQ=255)                                                                                                           ggTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCcg                              >  1:417051/1‑140 (MQ=255)                                                                                                           ggTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCcg                              >  2:44833/1‑140 (MQ=255)                                                                                                           ggTAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCcg                              >  2:163952/1‑140 (MQ=255)                                                                                                            gTAATATGGCGTCGGGCTTCATCATCAGAAAG‑GGGGGCCTGACGAATGAAAAAGTGAACAACAGTCAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCcgc                             <  2:938671/140‑1 (MQ=255)                                                                                                             tAATATGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCcgcg                            <  2:107300/140‑1 (MQ=255)                                                                                                                  tGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGaa                       >  2:853740/1‑140 (MQ=255)                                                                                                                  tGGCGTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGaa                       >  2:153707/1‑140 (MQ=255)                                                                                                                      gTCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTg                   <  2:1032008/140‑1 (MQ=255)                                                                                                                       tCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGc                  >  1:1028805/1‑140 (MQ=255)                                                                                                                       tCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGc                  >  2:527902/1‑140 (MQ=255)                                                                                                                       tCAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGc                  >  2:637900/1‑140 (MQ=255)                                                                                                                        cAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACGACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGcc                 <  2:112650/140‑1 (MQ=255)                                                                                                                        cAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGcc                 <  2:1065026/140‑1 (MQ=255)                                                                                                                        cAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGcc                 <  2:233731/140‑1 (MQ=255)                                                                                                                        cAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGcc                 <  1:418342/140‑1 (MQ=255)                                                                                                                        cAGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGcc                 <  1:697494/140‑1 (MQ=255)                                                                                                                         aGGCTTCATCATCAGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCa                >  1:337993/1‑140 (MQ=255)                                                                                                                              tcatcatcaGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATc           >  2:608704/1‑140 (MQ=255)                                                                                                                               catcatcaGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCa          <  1:5949/140‑1 (MQ=255)                                                                                                                                atcatcaGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCAt         <  1:44833/140‑1 (MQ=255)                                                                                                                                     caGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCt                    <  2:811346/124‑1 (MQ=255)                                                                                                                                     caGAAAG‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCt                    >  1:811346/1‑124 (MQ=255)                                                                                                                                      aGAAAG‑GGGGGCCTGACGCCTG‑AAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCa  <  2:1032798/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         TGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATCATCAGAAA‑‑GGGGGCCTGACGCCTGAAAAAGTGAACAACAGACAGTGTTCGGATTATCAATATTCAGTTTTTAACGTTGAACTGGATTCATTACTGCGTTGTTTAAGAATTTCCCGCGCCGAATCTGCCATTATCATGACTGCA  >  NC_000913/317541‑317813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑