Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	8,322	C→T	P29S (CCG→TCG)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	8,322	0	C	T	100.0%	217.6 / NA	63	P29S (CCG→TCG)	talB	transaldolase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/34);  total (29/34)

GGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACG  >  NC_000913/8188‑8454                                                                                                                                       |                                                                                                                                     ggCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAg                                                                                                                                 <  1:150112/140‑1 (MQ=255)    aGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGAt                                                                                                                              >  1:4218/1‑140 (MQ=255)    aGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGAt                                                                                                                              >  1:338906/1‑140 (MQ=255)      aCCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGc                                                                                                                            >  2:204592/1‑140 (MQ=255)      aCCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGc                                                                                                                            >  1:332008/1‑140 (MQ=255)       ccGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGcc                                                                                                                           <  1:638209/140‑1 (MQ=255)           ttACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACCTCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCAcaa                                                                                                                       >  2:231496/1‑140 (MQ=255)                  ccccTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAAccc                                                                                                                <  2:430315/140‑1 (MQ=255)                        aCAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTtctct                                                                                                          >  2:632150/1‑140 (MQ=255)                           aGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCAt                                                                                                       >  2:555175/1‑140 (MQ=255)                           aGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCAt                                                                                                       >  1:446292/1‑140 (MQ=255)                              tGTTTAAAGAGTAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCt                                                                                                    >  2:625957/1‑140 (MQ=255)                              tGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCt                                                                                                    <  1:530900/140‑1 (MQ=255)                                       agaAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAAcgcagc                                                                                           >  1:799177/1‑140 (MQ=255)                                        gaAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAGTCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAAcgcagcg                                                                                          <  1:347347/140‑1 (MQ=255)                                          aaTACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAAcgcagcgca                                                                                        >  2:480077/1‑140 (MQ=255)                                          aaTACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAAcgcagcgca                                                                                        <  1:330824/140‑1 (MQ=255)                                          aaTACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAAcgcagcgca                                                                                        <  1:656680/140‑1 (MQ=255)                                              cTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAAcgcagcgca                                                                                        >  1:824332/1‑136 (MQ=255)                                              cTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAAcgcagcgca                                                                                        <  2:824332/136‑1 (MQ=255)                                              cTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGAtt                                                                                    <  2:4218/140‑1 (MQ=255)                                                 tCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCg                                                                                 >  2:120020/1‑140 (MQ=255)                                                   aTGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGa                                                                               <  1:297404/140‑1 (MQ=255)                                                   aTGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGa                                                                               >  1:542116/1‑140 (MQ=255)                                                         gACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCGGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGa                                                                               >  1:750859/1‑134 (MQ=255)                                                         gACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGa                                                                               <  2:750859/134‑1 (MQ=255)                                                         gACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCg                                                                         >  2:948575/1‑140 (MQ=255)                                                         gACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCg                                                                         <  2:120584/140‑1 (MQ=255)                                                         gACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCg                                                                         >  1:353462/1‑140 (MQ=255)                                                           cAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTa                                                                       >  1:254677/1‑140 (MQ=255)                                                             aaTTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAg                                                                     <  2:799177/140‑1 (MQ=255)                                                                tGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTAGCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGttg                                                                  <  1:30086/140‑1 (MQ=255)                                                                       ccTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATtgatg                                                           >  1:290703/1‑140 (MQ=255)                                                                                 tACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGttgat                                                                >  1:321098/1‑125 (MQ=255)                                                                                 tACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGttgat                                                                <  2:321098/125‑1 (MQ=255)                                                                                 tACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGc                                                 <  2:353462/140‑1 (MQ=255)                                                                                    accaccGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTg                                              >  2:653389/1‑140 (MQ=255)                                                                                           tAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGaaa                                       <  1:324305/140‑1 (MQ=255)                                                                                           tAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGaaa                                       <  2:254677/140‑1 (MQ=255)                                                                                           tAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGaaa                                       >  2:1006130/1‑140 (MQ=255)                                                                                           tAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCGTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGaaa                                       >  2:478354/1‑140 (MQ=255)                                                                                           tAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTAGCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGaaa                                       <  2:542116/140‑1 (MQ=255)                                                                                              tGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAAcag                                    <  1:478354/140‑1 (MQ=255)                                                                                               ggCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAAcagc                                   <  1:225814/140‑1 (MQ=255)                                                                                               ggCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAAcagc                                   <  2:338906/140‑1 (MQ=255)                                                                                               ggCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAAcagc                                   <  1:480077/140‑1 (MQ=255)                                                                                                gCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAAcagca                                  <  2:840214/140‑1 (MQ=255)                                                                                                gCCGACACTGGGGACATCGCGGCAATGAAGCTGGATCAATCGCAGGATGCCACAACCAACCATTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAAcagca                                  >  2:970514/1‑140 (MQ=255)                                                                                                  cGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAAcag                                    <  2:199936/136‑1 (MQ=255)                                                                                                  cGACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAAcag                                    >  1:199936/1‑136 (MQ=255)                                                                                                   gACACTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCagag                               >  2:321297/1‑140 (MQ=255)                                                                                                     cacTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCa                             >  1:401017/1‑140 (MQ=255)                                                                                                      acTGGGGACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCaa                            <  1:948575/140‑1 (MQ=255)                                                                                                           ggACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATc                       <  2:470036/140‑1 (MQ=255)                                                                                                           ggACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATc                       <  2:855778/140‑1 (MQ=255)                                                                                                           ggACATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATc                       <  1:204592/140‑1 (MQ=255)                                                                                                             aCATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATcgc                     <  1:120020/140‑1 (MQ=255)                                                                                                              cATCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATcgcg                    <  1:321297/140‑1 (MQ=255)                                                                                                               aTCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATcgcgc                   <  1:625957/140‑1 (MQ=255)                                                                                                               aTCGCGGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATcgcgc                   >  1:416873/1‑140 (MQ=255)                                                                                                                  gcgGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATcgcg                    >  2:626476/1‑136 (MQ=255)                                                                                                                  gcgGCAATGAAGCTGTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATcgcg                    <  1:626476/136‑1 (MQ=255)                                                                                                                                gTATCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGAcg  <  1:653389/140‑1 (MQ=255)                                                                                                                                   tCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCg        >  1:186035/1‑131 (MQ=255)                                                                                                                                   tCAATCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCg        <  2:186035/131‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     GGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACG  >  NC_000913/8188‑8454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑