Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,403,790	C→T	R103* (CGA→TGA)	yhdH →	acrylyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,403,790	0	C	T	100.0%	162.3 / NA	49	R103* (CGA→TGA)	yhdH	acrylyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/28);  total (21/28)

CCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGG  >  NC_000913/3403655‑3403922                                                                                                                                        |                                                                                                                                     ccGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGt                                                                                                                                  <  2:376972/140‑1 (MQ=255)       atTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAgg                                                                                                                            >  1:262969/1‑140 (MQ=255)                 tCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGActggctg                                                                                                                  >  2:852676/1‑140 (MQ=255)                   gATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGt                                                                                                                >  1:166235/1‑140 (MQ=255)                   gATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGt                                                                                                                >  2:839887/1‑140 (MQ=255)                    aTTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGtt                                                                                                               >  2:717559/1‑140 (MQ=255)                     ttttGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTg                                                                                                              <  1:839887/140‑1 (MQ=255)                        tGCCGGAACTGTACGCCCCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCa                                                                                                           <  1:343582/140‑1 (MQ=255)                        tGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGTGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCa                                                                                                           <  1:245835/140‑1 (MQ=255)                             gAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCg                                                                                                      <  2:166235/140‑1 (MQ=255)                               aCTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCa                                                                                                    <  2:262969/140‑1 (MQ=255)                               aCTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCa                                                                                                    <  1:403607/140‑1 (MQ=255)                               aCTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCa                                                                                                    <  2:31532/140‑1 (MQ=255)                                  gTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAgg                                                                                                 >  1:968199/1‑140 (MQ=255)                                   tACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAggg                                                                                                >  1:833948/1‑140 (MQ=255)                                        aCCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGa                                                                                           >  1:223698/1‑140 (MQ=255)                                             cGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCg                                                                                                      >  2:794693/1‑124 (MQ=255)                                             cGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCg                                                                                                      <  1:794693/124‑1 (MQ=255)                                             cGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGAcgcgc                                                                                      >  1:28886/1‑140 (MQ=255)                                             cGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGAc                                                                                          >  1:71781/1‑136 (MQ=255)                                             cGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGAc                                                                                          <  2:71781/136‑1 (MQ=255)                                                aGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTa                                                                                   <  1:954355/140‑1 (MQ=255)                                                     cgcgTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCa                                                                              <  1:504493/140‑1 (MQ=255)                                                      gcgTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCaa                                                                             <  2:223698/140‑1 (MQ=255)                                                       cgTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAAt                                                                            >  1:225607/1‑140 (MQ=255)                                                        gTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATg                                                                           <  2:750232/140‑1 (MQ=255)                                                             cATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTAt                                                                      >  1:1019760/1‑140 (MQ=255)                                                              aTGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATc                                                                     >  2:123679/1‑140 (MQ=255)                                                                gCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCgg                                                                   <  1:852676/140‑1 (MQ=255)                                                                  cGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTa                                                                 <  1:717559/140‑1 (MQ=255)                                                                       aggaggTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGcc                                                            <  1:123679/140‑1 (MQ=255)                                                                             tgtTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCg                                                                    <  2:829796/126‑1 (MQ=255)                                                                             tgtTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCg                                                                    >  1:829796/1‑126 (MQ=255)                                                                                  cTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGc                                                 <  2:28886/140‑1 (MQ=255)                                                                                  cTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGc                                                 <  2:419899/140‑1 (MQ=255)                                                                                   tCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGcc                                                <  2:225607/140‑1 (MQ=255)                                                                                      ctggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATg                                             <  2:1019760/140‑1 (MQ=255)                                                                                      ctggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATg                                             <  1:462127/140‑1 (MQ=255)                                                                                      ctggctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCAATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATg                                             <  2:614947/140‑1 (MQ=255)                                                                                         gctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGttt                                                       <  2:965824/127‑1 (MQ=255)                                                                                         gctggGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGttt                                                       >  1:965824/1‑127 (MQ=255)                                                                                             gggCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCtgtgtg                                      >  1:8592/1‑140 (MQ=255)                                                                                                   tGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATgg                                >  2:940851/1‑140 (MQ=255)                                                                                                     gTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGgcg                              <  1:346442/140‑1 (MQ=255)                                                                                                        aaaaCCACTGGGGTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTg                           >  2:437744/1‑140 (MQ=255)                                                                                                                  gggTGGGCTGGCGGAGCAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGATGTGTGTGATGGCGCTGGAAGATGCCg                 >  2:389328/1‑140 (MQ=255)                                                                                                                              ggAGCAGGCGTGAGTAAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGAAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGc     <  1:940851/140‑1 (MQ=255)                                                                                                                                  cAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAgg  <  2:209936/139‑1 (MQ=255)                                                                                                                                  cAGGCGTGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAgg  >  1:209936/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                     CCGATGATTCCTGGGATCGATTTTGCCGGAACTGTACGCACCAGCGAAGATCCGCGTTTTCATGCCGGTCAGGAGGTGTTACTCACTGGCTGGGGCGTTGGTGAAAACCACTGGGGTGGGCTGGCGGAGCAGGCGCGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGG  >  NC_000913/3403655‑3403922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑