Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,405,687	T→C	R84R (CGT→CGC)	accB →	biotin carboxyl carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,405,687	0	T	C	100.0%	188.1 / NA	52	R84R (CGT→CGC)	accB	biotin carboxyl carrier protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/25);  total (27/25)

TGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGTTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCG  >  NC_000913/3405561‑3405822                                                                                                                               |                                                                                                                                        tgtgATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGAtggttg                                                                                                                            <  1:136362/140‑1 (MQ=255) tgtgATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGAtggttg                                                                                                                            <  1:869802/140‑1 (MQ=255) tgtgATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGAtggttg                                                                                                                            <  1:636608/140‑1 (MQ=255)    gATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTa                                                                                                                         <  2:159815/140‑1 (MQ=255)    gATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTa                                                                                                                         >  2:207486/1‑140 (MQ=255)    gATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTa                                                                                                                         <  1:651320/140‑1 (MQ=255)               cTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGc                                                                                                              >  2:292769/1‑140 (MQ=255)                        cACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGc                                                                                                     >  2:291302/1‑140 (MQ=255)                                  gcagcaGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCaa                                                                                           >  1:104183/1‑140 (MQ=255)                                  gcagcaGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCaa                                                                                           >  2:1059352/1‑140 (MQ=255)                                       agccagcTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCg                                                                                      <  1:1059352/140‑1 (MQ=255)                                        gccagcTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGt                                                                                     >  2:408763/1‑140 (MQ=255)                                        gccagcTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGt                                                                                     >  2:264125/1‑140 (MQ=255)                                        gccagcTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGt                                                                                     >  2:82030/1‑140 (MQ=255)                                        gccagcTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGt                                                                                     >  1:32269/1‑140 (MQ=255)                                           agcTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGc                                                                                       >  2:797258/1‑135 (MQ=255)                                           agcTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGc                                                                                       <  1:797258/135‑1 (MQ=255)                                                aaTCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGaa                                                                             <  1:408763/140‑1 (MQ=255)                                                aaTCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGaa                                                                             >  1:205002/1‑140 (MQ=255)                                                aaTCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGaa                                                                             <  1:207486/140‑1 (MQ=255)                                                aaTCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGaa                                                                             <  2:32269/140‑1 (MQ=255)                                                        gCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCa                                                                     <  2:104183/140‑1 (MQ=255)                                                            ccGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGaaa                                                                 >  2:644181/1‑140 (MQ=255)                                                            ccGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGaaa                                                                 >  2:419373/1‑140 (MQ=255)                                                            ccGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGaaa                                                                 >  2:682851/1‑140 (MQ=255)                                                                  cGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAAc                                                           <  2:205002/140‑1 (MQ=255)                                                                   ggCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACg                                                          <  1:419373/140‑1 (MQ=255)                                                                    gCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGt                                                         <  1:82030/140‑1 (MQ=255)                                                                    gCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGt                                                         <  2:210951/140‑1 (MQ=255)                                                                    gCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGt                                                         >  2:187918/1‑140 (MQ=255)                                                                    gCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGt                                                         >  2:42708/1‑140 (MQ=255)                                                                      gACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTgg                                                       >  2:394221/1‑140 (MQ=255)                                                                         cGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCg                                                    <  1:42708/140‑1 (MQ=255)                                                                           ttccttccATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGAt                                                  <  1:658577/140‑1 (MQ=255)                                                                              cttccATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATAcc                                               <  2:787182/140‑1 (MQ=255)                                                                                 ccATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCtg                                            >  1:58268/1‑140 (MQ=255)                                                                                  cATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCtgt                                           >  1:942819/1‑140 (MQ=255)                                                                                   aTGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCtgtg                                          <  2:942819/140‑1 (MQ=255)                                                                                     ggAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCa                                        <  2:509526/140‑1 (MQ=255)                                                                                     ggAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCa                                        <  1:291302/140‑1 (MQ=255)                                                                                          cgcCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGtt                                   >  2:142593/1‑140 (MQ=255)                                                                                          cgcCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGtt                                   >  2:21737/1‑140 (MQ=255)                                                                                           gcCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTg                                  >  2:205683/1‑140 (MQ=255)                                                                                                 agcagcGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCa                            <  1:394221/140‑1 (MQ=255)                                                                                                     gcGGAAATCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGaa                        <  1:292769/140‑1 (MQ=255)                                                                                                           aTCAGTGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAatgat                  <  1:187918/140‑1 (MQ=255)                                                                                                                tGGTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTCGAAGCCATGAAAATGATGAAcc             >  1:1053627/1‑140 (MQ=255)                                                                                                                  gTCACATCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAg           >  1:223285/1‑140 (MQ=255)                                                                                                                       aTCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGa      >  2:353490/1‑140 (MQ=255)                                                                                                                        tCGTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACACAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGaa     >  2:151872/1‑140 (MQ=255)                                                                                                                          gTACGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGc   >  2:549753/1‑140 (MQ=255)                                                                                                                            aCGCTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCg  <  1:855838/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                        TGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGTTCCTTCCATGGAAGCGCCAGCAGCAGCGGAAATCAGTGGTCACATCGTACGTTCCCCGATGGTTGGTACTTTCTACCGCACCCCAAGCCCGGACGCAAAAGCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCG  >  NC_000913/3405561‑3405822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑