Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,421,409	T→C	F29L (TTC→CTC)	yhdY →	putative ABC transporter membrane subunit YhdY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,421,409	0	T	C	100.0%	239.1 / NA	66	F29L (TTC→CTC)	yhdY	putative ABC transporter membrane subunit YhdY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/30);  total (36/30)

GATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCG  >  NC_000913/3421273‑3421544                                                                                                                                         |                                                                                                                                        gatgaATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGctct                                                                                                                                      >  1:1058029/1‑140 (MQ=255)            ataACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGc                                                                                                                           >  2:509860/1‑140 (MQ=255)                 cgccgcATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAg                                                                                                                      >  1:718051/1‑140 (MQ=255)                   ccgcATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGcc                                                                                                                    >  1:559071/1‑140 (MQ=255)                   ccgcATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGcc                                                                                                                    >  2:899720/1‑140 (MQ=255)                   ccgcATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGcc                                                                                                                    >  1:947762/1‑140 (MQ=255)                   ccgcATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGcc                                                                                                                    >  1:950322/1‑140 (MQ=255)                   ccgcATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCG‑AAAAATCTGCTCTCCAGc                                                                                                                                 >  1:879358/1‑126 (MQ=255)                   ccgcATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCG‑AAAAATCTGCTCTCCAGc                                                                                                                                 <  2:879358/126‑1 (MQ=255)                   ccgcATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGT‑GGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCct                                                                                                                   >  1:715916/1‑140 (MQ=255)                    cgcATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCct                                                                                                                   <  1:1011655/140‑1 (MQ=255)                    cgcATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCct                                                                                                                   >  1:252630/1‑140 (MQ=255)                    cgcATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCct                                                                                                                   >  2:406803/1‑140 (MQ=255)                      cATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCctgc                                                                                                                 <  2:559071/140‑1 (MQ=255)                      cATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCctgc                                                                                                                 <  1:601510/140‑1 (MQ=255)                       aTCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCctgct                                                                                                                <  1:1016543/140‑1 (MQ=255)                            gATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTa                                                                                                           <  1:261439/140‑1 (MQ=255)                                     aCGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCa                                                                                                  <  2:876385/140‑1 (MQ=255)                                     aCGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCa                                                                                                  <  1:899720/140‑1 (MQ=255)                                      cGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCat                                                                                                 <  2:718051/140‑1 (MQ=255)                                        cTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTgg                                                                                                       <  2:268565/132‑1 (MQ=255)                                        cTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTgg                                                                                                       >  1:268565/1‑132 (MQ=255)                                        cTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCatat                                                                                               >  1:502934/1‑140 (MQ=255)                                        cTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCatat                                                                                               >  1:700500/1‑140 (MQ=255)                                         tAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTAt                                                                                                          <  2:394427/128‑1 (MQ=255)                                         tAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTAt                                                                                                          >  1:394427/1‑128 (MQ=255)                                         tAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATg                                                                                              <  1:765416/140‑1 (MQ=255)                                          aaGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATgg                                                                                             >  1:711125/1‑140 (MQ=255)                                           aGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGt                                                                                            <  2:700500/140‑1 (MQ=255)                                              aGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGa                                                                                         <  2:415848/140‑1 (MQ=255)                                              aGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGT‑GGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGAt                                                                                        <  2:715916/140‑1 (MQ=255)                                                tCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATgg                                                                                             >  2:353713/1‑134 (MQ=255)                                                tCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATgg                                                                                             <  1:353713/134‑1 (MQ=255)                                                 cATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCa                                                                                                  >  1:893526/1‑128 (MQ=255)                                                 cATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCa                                                                                                  <  2:893526/128‑1 (MQ=255)                                                     atgaCAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGa                                                                                  >  2:596559/1‑140 (MQ=255)                                                       gaCAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGtt                                                                                           >  2:789557/1‑129 (MQ=255)                                                       gaCAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGtt                                                                                           <  1:789557/129‑1 (MQ=255)                                                       gaCAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAAc                                                                                >  2:676689/1‑140 (MQ=255)                                                       gaCAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAAc                                                                                >  2:87113/1‑140 (MQ=255)                                                          aaaaGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGa                                                                             >  2:1065486/1‑140 (MQ=255)                                                            aaGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGCGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATc                                                                           >  2:1055149/1‑140 (MQ=255)                                                             aGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATcc                                                                          <  1:161032/140‑1 (MQ=255)                                                                aTTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCAc                                                                       <  2:252630/140‑1 (MQ=255)                                                                     gatctcTTCCCCCGCGCCGGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTg                                                                  <  2:947762/138‑1 (MQ=255)                                                                         tcATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGa                                                              >  1:341085/1‑140 (MQ=255)                                                                            tCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCAc                                                                       <  1:93911/128‑1 (MQ=255)                                                                            tCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCAc                                                                       >  2:93911/1‑128 (MQ=255)                                                                            tCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACt                                                           >  1:273490/1‑140 (MQ=255)                                                                            tCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACt                                                           >  2:666316/1‑140 (MQ=255)                                                                                       cGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTg                                                <  2:502934/140‑1 (MQ=255)                                                                                       cGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTg                                                <  2:1058029/140‑1 (MQ=255)                                                                                                    cTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTggg                                   <  2:341085/140‑1 (MQ=255)                                                                                                    cTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTggg                                   <  1:596559/140‑1 (MQ=255)                                                                                                      cAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGtt                                 <  1:406803/140‑1 (MQ=255)                                                                                                        aGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTgg                               >  2:100838/1‑140 (MQ=255)                                                                                                         gCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGc                              >  1:244210/1‑140 (MQ=255)                                                                                                         gCCGCGCGATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGc                              <  1:100838/140‑1 (MQ=255)                                                                                                            gcgcgATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCa                           >  1:527696/1‑140 (MQ=255)                                                                                                             cgcgATGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCaa                          >  2:1083934/1‑140 (MQ=255)                                                                                                                  tGGTGTGGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGt                     <  2:244210/140‑1 (MQ=255)                                                                                                                     tgtgGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGcc                  <  2:192909/140‑1 (MQ=255)                                                                                                                     tgtgGGTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGcc                  <  1:245227/140‑1 (MQ=255)                                                                                                                          gTGCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTg             >  1:399554/1‑140 (MQ=255)                                                                                                                            gCGAAAAAATCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGca           >  2:256806/1‑140 (MQ=255)                                                                                                                               aaaaaaTCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACaa        >  2:117997/1‑140 (MQ=255)                                                                                                                               aaaaaaTCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACaa        >  2:385268/1‑140 (MQ=255)                                                                                                                                     tCTGCTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCg  >  1:978673/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCATCCCCCGCGCCCGGCGAGCCATAACTCAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGCAGGCTAACTGGGTTGGCTCAACGCGTGCCGACTGCACAAAAGCCG  >  NC_000913/3421273‑3421544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑