Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,438,502	T→C	N50D (AAC→GAC)	arfA ←	alternative ribosome‑rescue factor A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,438,502	0	T	C	100.0%	287.4 / NA	80	N50D (AAC→GAC)	arfA	alternative ribosome‑rescue factor A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/43);  total (37/43)

ACCAACTAAAGAAGAAGTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTTCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTATCGCATTATCCTTTATCTGCCCTTTAGTATG  >  NC_000913/3438365‑3438634                                                                                                                                          |                                                                                                                                     aCCAACTAAAGAAGAAGTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTccc                                                                                                                                    >  2:55910/1‑140 (MQ=255)   cAACTAAAGAAGAAGTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCcccc                                                                                                                                  <  1:648970/140‑1 (MQ=255)     aCTAAAGAAGAAGTATTGCTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCg                                                                                                                                >  2:288999/1‑140 (MQ=255)     aCTAAAGAAGAAGTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCg                                                                                                                                >  2:979245/1‑140 (MQ=255)     aCTAAAGAAGAAGTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCg                                                                                                                                >  1:621447/1‑140 (MQ=255)     aCTAAAGAAGAAGTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCg                                                                                                                                >  2:764317/1‑140 (MQ=255)     aCTAAAGAAGAAGTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCg                                                                                                                                >  2:138895/1‑140 (MQ=255)     aCTAAAGAAGAAGTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCg                                                                                                                                >  2:20115/1‑140 (MQ=255)      cTAAAGAAGAAGTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGa                                                                                                                               <  2:292613/140‑1 (MQ=255)           gaagaagTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGcc                                                                                                                          <  1:138895/140‑1 (MQ=255)           gaagaagTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGcc                                                                                                                          <  1:283709/140‑1 (MQ=255)             agaagTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCAt                                                                                                                        >  2:444843/1‑140 (MQ=255)               aagTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGcc                                                                                                                          >  2:1056129/1‑136 (MQ=255)               aagTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGcc                                                                                                                          <  1:1056129/136‑1 (MQ=255)                agTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGtt                                                                                                                     <  2:621447/140‑1 (MQ=255)                 gTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGttt                                                                                                                    >  2:734520/1‑140 (MQ=255)                 gTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGttt                                                                                                                    >  2:58463/1‑140 (MQ=255)                          aCAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCg                                                                                                           >  1:463583/1‑140 (MQ=255)                           cAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGc                                                                                                          <  1:444843/140‑1 (MQ=255)                           cAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGc                                                                                                          <  1:979245/140‑1 (MQ=255)                           cAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGc                                                                                                          >  1:831895/1‑140 (MQ=255)                           cAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGc                                                                                                          <  1:734520/140‑1 (MQ=255)                            aGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCa                                                                                                         >  2:251678/1‑140 (MQ=255)                            aGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCa                                                                                                         >  2:631437/1‑140 (MQ=255)                              aaaTTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATg                                                                                                       >  1:4172/1‑140 (MQ=255)                                 ttCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTaa                                                                                                    <  1:288999/140‑1 (MQ=255)                                 ttCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTaa                                                                                                    >  1:807671/1‑140 (MQ=255)                                 ttCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTaa                                                                                                    >  1:534579/1‑140 (MQ=255)                                    gTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTg                                                                                                 <  2:807671/140‑1 (MQ=255)                                      gATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGcc                                                                                               >  2:679765/1‑140 (MQ=255)                                          tGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTc                                                                                           >  1:739943/1‑140 (MQ=255)                                                     cAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTAtt                                                                                <  2:951516/140‑1 (MQ=255)                                                         aTAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTtc                                                                            >  2:997577/1‑140 (MQ=255)                                                             ccGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTa                                                                        >  1:747487/1‑140 (MQ=255)                                                               gCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTAcg                                                                      <  2:739943/140‑1 (MQ=255)                                                                ctctTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTAcgc                                                                     <  1:617987/140‑1 (MQ=255)                                                                 tctTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTAcgcg                                                                    >  1:633880/1‑140 (MQ=255)                                                                 tctTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTAcgcg                                                                    <  2:747487/140‑1 (MQ=255)                                                                 tctTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTAcgcg                                                                    <  2:633880/140‑1 (MQ=255)                                                                     aaCAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGt                                                                <  2:463583/140‑1 (MQ=255)                                                                     aaCAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGt                                                                <  1:218047/140‑1 (MQ=255)                                                                       cAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCg                                                              <  2:1083053/140‑1 (MQ=255)                                                                       cAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCg                                                              <  2:975264/140‑1 (MQ=255)                                                                           cgcCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAAt                                                          >  1:869020/1‑140 (MQ=255)                                                                             ccTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTAcgcg                                                                    >  1:531709/1‑128 (MQ=255)                                                                             ccTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTAcgcg                                                                    <  2:531709/128‑1 (MQ=255)                                                                             ccTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATaa                                                        >  1:347953/1‑140 (MQ=255)                                                                             ccTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATaa                                                        >  2:775540/1‑140 (MQ=255)                                                                              cTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAg                                                       <  1:58463/140‑1 (MQ=255)                                                                              cTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAg                                                       <  2:4172/140‑1 (MQ=255)                                                                               tGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTAcgcg                                                                    <  1:47553/126‑1 (MQ=255)                                                                               tGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTAcgcg                                                                    >  2:47553/1‑126 (MQ=255)                                                                               tGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAgg                                                      >  1:909492/1‑140 (MQ=255)                                                                               tGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAgg                                                      >  1:913055/1‑140 (MQ=255)                                                                                gAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAggg                                                     <  1:631437/140‑1 (MQ=255)                                                                                 aaaGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGa                                                    <  1:20115/140‑1 (MQ=255)                                                                                 aaaGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGa                                                    <  2:831895/140‑1 (MQ=255)                                                                                 aaaGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGa                                                    <  2:42682/140‑1 (MQ=255)                                                                                 aaaGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGa                                                    <  2:141378/140‑1 (MQ=255)                                                                                 aaaGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGa                                                    <  1:997577/140‑1 (MQ=255)                                                                                   aGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATc                                                  <  2:534579/140‑1 (MQ=255)                                                                                   aGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATc                                                  <  2:913055/140‑1 (MQ=255)                                                                                   aGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATc                                                  <  2:909492/140‑1 (MQ=255)                                                                                        aagaCCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATa                                                         >  2:1059938/1‑128 (MQ=255)                                                                                        aagaCCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATa                                                         <  1:1059938/128‑1 (MQ=255)                                                                                             cAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGa                                                    >  2:522373/1‑128 (MQ=255)                                                                                             cAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGa                                                    <  1:522373/128‑1 (MQ=255)                                                                                               gTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGc                                      <  1:775540/140‑1 (MQ=255)                                                                                                     aaaaaGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTAt                                >  1:432020/1‑140 (MQ=255)                                                                                                      aaaaGTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTATc                               <  2:347953/140‑1 (MQ=255)                                                                                                          gTGATTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTATCGCAt                           <  1:679765/140‑1 (MQ=255)                                                                                                             aTTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTATCGCATTAt                        <  1:860556/140‑1 (MQ=255)                                                                                                             aTTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTATCGCATTAt                        >  2:787411/1‑140 (MQ=255)                                                                                                             aTTTACTTTCTTGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTATCGCATTAt                        >  1:144461/1‑140 (MQ=255)                                                                                                                        tGCCACTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTATCGCATTATCCTTTATCTGc             >  2:363444/1‑140 (MQ=255)                                                                                                                            aCTGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTATCGCATTATCCTTTATCTGCCCtt         >  2:402249/1‑140 (MQ=255)                                                                                                                              tGGCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTATCGCATTATCCTTTATCTGCCCTTTa       <  2:869020/140‑1 (MQ=255)                                                                                                                               ggCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTATCGCATTATCCTTTATCTGCCCTTTAg      >  2:57724/1‑140 (MQ=255)                                                                                                                                gCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTATCGCATTATCCTTTATCTGCCCTTTAGt     <  1:764317/140‑1 (MQ=255)                                                                                                                                gCCTCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTATCGCATTATCCTTTATCTGCCCTTTAGt     <  1:57724/140‑1 (MQ=255)                                                                                                                                   tCCCAGTCCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTATCGCATTATCCTTTATCTGCCCTTTAGTATg  <  1:363444/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     ACCAACTAAAGAAGAAGTATTACTGACAGAAATTCGTGATTTGCTGAAAGAGCAGAATAACCGCTCTTAACAAGCGCCTGAAAGCAGAAGACCAGTGGTAAAAAAGTGATTTACTTTCTTGCCACTGGCCTCCCAGTTCCCCCGATTGCCATGTTTTCCTTTTCGCATGTAACTGCCTTTCCCCTTCTTATTTTTCTCTACGCGCTGTCGGAATAAGGGATCATGTAGTAATGCTTCTATCGCATTATCCTTTATCTGCCCTTTAGTATG  >  NC_000913/3438365‑3438634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑