Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,468,938	T→C	T306A (ACT→GCT)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,468,938	0	T	C	100.0%	121.7 / NA	35	T306A (ACT→GCT)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/4);  total (31/4)

AAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCAT  >  NC_000913/3468809‑3469063                                                                                                                                  |                                                                                                                              aattttCGCTCCCTTCCTGGCCGCTCCACGCTTGAAATGAGTAATCGTTAACGCTACCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTGGGGGTCCCGGGTGTTTCCGGAGTTGGATCACCCGGAGCCACAGCACCg                                                                                                                     <  2:110534/137‑1 (MQ=255)         cTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCattatt                                                                                                             >  1:110534/1‑140 (MQ=255)               tCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCgg                                                                                                       >  1:1001545/1‑140 (MQ=255)                        cTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGa                                                                                              >  1:842791/1‑140 (MQ=255)                         tCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGaa                                                                                             >  2:543306/1‑140 (MQ=255)                         tCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGaa                                                                                             >  1:450280/1‑140 (MQ=255)                          ccACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCACCGGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAAc                                                                                            <  2:619443/140‑1 (MQ=255)                              gCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGa                                                                                        >  2:457742/1‑140 (MQ=255)                               cTTGCAATGAGTAATCGTTAACGCTTCCCGGTGGTGTTGATGGCTCGCTGTTACCCGGAGTTGGGGTCCCCGGTTTTTCGGGAGTTGGACCACCGGGAGCCCCAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAg                                                                                       <  2:842791/140‑1 (MQ=255)                               cTTGCAATGAGAATTCGTTAGCGTTACCCGCGGGTGTTAGGGGCTCGTTGTTACCCGGGGTGGGGTGCACCGTTGTTCCGGGATTTGGATCACCGGGACCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAg                                                                                       <  2:450280/140‑1 (MQ=255)                                 tGCAATGAGTAATCGTAACCGCTATCCGCTGGTGTTGATGGCTCGCTTTTACCGGGGGTGGGGGTCCCGGGTTTTTCGGGAGTTGGACCAACCGGACCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGcc                                                                                     <  2:865987/140‑1 (MQ=255)                                  gaaaTGAGTAATCGTTAACGCTACCCGCTGGTTTTGAGGGCTCGCTTTTATCCGGAGTGGGGGTCACGGGTTTTCCGGGAGTTGGACCACCGGGACCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCa                                                                                    <  2:354163/138‑1 (MQ=255)                                   aaaTGAGTAATCGTTAACGCTACCCGTTGGTGTTGATGGCTCGCTTTTATCGGGAGTGGGGGTCCCCGGTTTTCCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAg                                                                                   <  2:429473/139‑1 (MQ=255)                                        aGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTa                                                                              >  1:865987/1‑140 (MQ=255)                                          tAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTAcc                                                                            >  2:1024057/1‑140 (MQ=255)                                                  aaCGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGc                                                                    >  1:834726/1‑140 (MQ=255)                                                    cGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTg                                                                  >  1:722190/1‑140 (MQ=255)                                                    cGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTg                                                                  >  2:156317/1‑140 (MQ=255)                                                        aTCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGAttt                                                              >  1:298827/1‑140 (MQ=255)                                                         tCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTc                                                             >  1:723777/1‑140 (MQ=255)                                                         tCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTc                                                             >  1:129925/1‑140 (MQ=255)                                                              tGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttg                                                        >  1:954461/1‑140 (MQ=255)                                                              tGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttg                                                        >  2:623088/1‑140 (MQ=255)                                                                 tgtTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttgccg                                                     >  1:112491/1‑140 (MQ=255)                                                                   ttGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttgccgtt                                                   >  1:568531/1‑140 (MQ=255)                                                                      aTGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTgttgccgttgcc                                                >  2:458002/1‑140 (MQ=255)                                                                         gCTCGCTTTTATCGGGAGTTGGGGTCCCGGGTGTTTCGGGAGTTGGATCACCGGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCcgcg                                             <  2:423134/140‑1 (MQ=255)                                                                          cTCGTTTTTATCGGGAGTGGGGGTCCCCGGTGTTTCGGGAGTTGGATCACCGGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCcgcgc                                            <  2:723777/140‑1 (MQ=255)                                                                          cTCGCTGTTATCGGGAGTGGGGGTCCCGGGTTTTCCCGGAGTTGGATCACCGGGACCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCcgcgc                                            <  2:568531/140‑1 (MQ=255)                                                                          cTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCcgcgc                                            >  2:118621/1‑140 (MQ=255)                                                                          cTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCcgcgc                                            >  1:441375/1‑140 (MQ=255)                                                                          cTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCcgcgc                                            >  1:423134/1‑140 (MQ=255)                                                                                tttATCGGGAGTGGGGGCCCCGGGTTTTTCCGGGGTGGGATCACCGGGCGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACAt                                      <  2:638299/139‑1 (MQ=255)                                                                                gTTATCGGGAGTGGGGGCCCCCGGTGTTCCCGGAGTTGGACCACCGGGACCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACAt                                      <  2:722190/140‑1 (MQ=255)                                                                                    ccgggAGTGGGGGTCACGGGTGTTCCCGGAGTTGGATCACCGGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACg                                  <  2:298827/137‑1 (MQ=255)                                                                                      cGGGGTTGGGGCCCCCGGTTTTTCCGGTTTTGGATCACCCGGGGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGcc                                <  2:112491/140‑1 (MQ=255)                                                                                       ggAGTGGGGGTCCCGGGTTTTCCGGGAGTGGGATCACCGGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCa                               <  2:1001545/140‑1 (MQ=255)                                                                                         aGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACAt                                      <  2:850469/131‑1 (MQ=255)                                                                                         aGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACAt                                      >  1:850469/1‑131 (MQ=255)                                                                                         aGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATg                             >  1:871239/1‑140 (MQ=255)                                                                                         aGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATg                             >  1:139562/1‑140 (MQ=255)                                                                                           ttGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATggg                           >  1:1052218/1‑140 (MQ=255)                                                                                           ttGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATggg                           >  1:797858/1‑140 (MQ=255)                                                                                           ttGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATggg                           >  1:252929/1‑140 (MQ=255)                                                                                              gggTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCG‑ACATAACGCCATGGGTTAc                       >  1:847130/1‑140 (MQ=255)                                                                                                    ccGGTTTTTCCGGAGTTGGATCACCGGGAGCCACCGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCa                  <  1:942554/140‑1 (MQ=39)                                                                                                           ttCCGGAGTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGtttt           >  2:583477/1‑140 (MQ=255)                                                                                                                  gTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGc    <  2:834726/140‑1 (MQ=255)                                                                                                                  gTTGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGc    <  1:156317/140‑1 (MQ=255)                                                                                                                    tGGATCAACCGGAGCCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGAATCGTTTTCTTTCGCAt  >  2:1003455/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                              AAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCAT  >  NC_000913/3468809‑3469063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑