Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,492,018	T→C	N94D (AAC→GAC)	ppiA ←	peptidyl‑prolyl cis‑trans isomerase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,492,018	0	T	C	100.0%	101.3 / NA	32	N94D (AAC→GAC)	ppiA	peptidyl‑prolyl cis‑trans isomerase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/27);  total (5/27)

GGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTTTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCT  >  NC_000913/3491883‑3492154                                                                                                                                        |                                                                                                                                          ggTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGggtctggt                                                                                                                                       <  2:17564/140‑1 (MQ=255)      aggaaggCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGtttttt                                                                                                                                  >  1:139580/1‑140 (MQ=255)         aaggCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTctg                                                                                                                               <  1:539894/140‑1 (MQ=255)           ggCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGGCTGGgttttttttct                                                                                                                             >  2:1027139/1‑135 (MQ=255)             cGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGGCTGGgtttttttttttt                                                                                                                           >  2:1081338/1‑133 (MQ=255)                tATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATc                                                                                                                        <  1:1027139/140‑1 (MQ=255)                  tCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTg                                                                                                                      <  2:646879/140‑1 (MQ=255)                   cGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGc                                                                                                                     <  1:128841/140‑1 (MQ=255)                               tAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAAccg                                                                                                        <  2:789707/140‑1 (MQ=255)                               tAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAAccg                                                                                                        <  1:392724/140‑1 (MQ=255)                                    aaCTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTGTGGGTTTTTTTTCTGCCTCTTCT‑CCGTGGAAACgccccc                                                                                                   >  2:546973/1‑140 (MQ=255)                                    aaCTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCgccgcc                                                                                                   <  1:164481/140‑1 (MQ=255)                                     aCTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGGCTGGGTTTTTTTTCTTCATCTTCT‑CGGTGGAAAcccccccc                                                                                                  >  1:1066990/1‑140 (MQ=255)                                         gctggTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGGCTGGGTTTTTTTTCTGCATCTTCTCCGGGGAAACCCCCCCCCGGa                                                                                               >  2:890930/1‑140 (MQ=255)                                              tggCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCTGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATa                                                                                         >  1:861604/1‑140 (MQ=255)                                                gcgcTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATaaa                                                                                       <  1:1081338/140‑1 (MQ=255)                                                             cAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcg                                                                          <  2:139580/140‑1 (MQ=255)                                                             cAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcg                                                                          <  1:385400/140‑1 (MQ=255)                                                             cAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcg                                                                          <  2:897640/140‑1 (MQ=255)                                                               gCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCTGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcgcg                                                                        <  2:861604/140‑1 (MQ=255)                                                                  gTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTg                                                                     <  1:546973/140‑1 (MQ=255)                                                                  gTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTg                                                                     <  2:584276/140‑1 (MQ=255)                                                                    aCGTGCCATCGCGATGGTGCCACGGGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGaa                                                                   <  1:668768/140‑1 (MQ=255)                                                                    aCGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGaa                                                                   <  2:712124/140‑1 (MQ=255)                                                                      gTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAg                                                                 <  1:959430/140‑1 (MQ=255)                                                                            tCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAgtg                                                           <  1:890930/140‑1 (MQ=255)                                                                            tCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAgtg                                                           <  2:888/140‑1 (MQ=255)                                                                            gacagATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAgtg                                                           <  1:1057019/136‑1 (MQ=255)                                                                              gcgATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGtgtt                                                         <  2:1030528/140‑1 (MQ=255)                                                                                       ccACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTGTGGGTTTTTTTTCTGCATCTGCT‑CGGTGGAAACCCCGCCCCGAATCATAAAACCAGGAAAGACGCGGGGAAAAGGAGGGTTGTTTTaaaa                                                >  2:151150/1‑140 (MQ=255)                                                                                          cgcgTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAAcc                                             <  1:48737/140‑1 (MQ=255)                                                                                           gcgTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCg                                            <  2:693154/140‑1 (MQ=255)                                                                                           gcgTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCg                                            <  2:758127/140‑1 (MQ=255)                                                                                            cgTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGGCTGGGTTTTTTTTCTTCCTCTGCT‑CGGGGGAAACGCCCCCCCGAATCATAAAACCAGGGAAGAAGCGGGGGAAAGGAGGGTTGTTTTaaaaatatt                                           >  2:1057019/1‑136 (MQ=255)                                                                                                             tATCGGCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCGACa                          <  1:218204/140‑1 (MQ=255)                                                                                                                  gCTTCATTTTTGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCGACAAAGtt                     <  1:666905/140‑1 (MQ=255)                                                                                                                    ttCATTTTTGATTGGCGGGTCTGGGTTTTTTTTCTGCCTCTTCT‑CCGTGAAAACCCCCCCCCCCATCATAAAAACAAGGAAGACGCGGGGGAAGGGAGGGGTGTTATAAAAAACCCCGGTTACATAAACGACaaaaattt                   >  2:218204/1‑135 (MQ=255)                                                                                                                      cATTTTTGATTGGCGGGTCTGGGTTTTTTTTCTGCATCTGCT‑CCGTGGAAACGCCGCCCCGGATCATAAAACCAGGGATGACGCGGGGGAAGGGAGTGGTGTTTTTAAAAACCCTGTTTACATAATCGACaaaaattttt                 >  2:666905/1‑133 (MQ=255)                                                                                                                         ttttGATTGGCGGGTCTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGcaca              <  2:1066990/140‑1 (MQ=255)                                                                                                                                     ggtctggtttTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCt  <  2:541175/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                          GGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGTTTGGTTTTTTCTGCTGCATCTGCT‑CGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCT  >  NC_000913/3491883‑3492154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑