Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,579,158	T→C	Y163C (TAC→TGC)	yhhW ←	quercetin 2,3‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,579,158	0	T	C	100.0%	309.5 / NA	85	Y163C (TAC→TGC)	yhhW	quercetin 2,3‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/39);  total (46/39)

CCCAGATTGCCAGACCATCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTTTCTTCAGGCATGATCCA  >  NC_000913/3579021‑3579294                                                                                                                                          |                                                                                                                                         cccAGATTGCCAGACCATCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCAc                                                                                                                                        >  1:541653/1‑140 (MQ=255)  ccAGATTGCCAGACCATCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGcaca                                                                                                                                       <  2:306623/140‑1 (MQ=255)  ccAGATTGCCAGACCATCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGcaca                                                                                                                                       <  2:342536/140‑1 (MQ=255)  ccAGATTGCCAGACCATCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGcaca                                                                                                                                       <  2:874594/140‑1 (MQ=255)       ttGCCAGACCATCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTcc                                                                                                                                  <  1:183/140‑1 (MQ=255)           cAGACCATCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCatat                                                                                                                              >  2:365683/1‑140 (MQ=255)           cAGACCATCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCatat                                                                                                                              >  2:479397/1‑140 (MQ=255)           cAGACCATCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCatat                                                                                                                              >  1:621985/1‑140 (MQ=255)           cAGACCATCGCTGGTCGAGGCTTTCACGCCGTTAATGATGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCatat                                                                                                                              >  1:1048721/1‑140 (MQ=255)            aGACCATCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATc                                                                                                                             <  2:716954/140‑1 (MQ=255)                cATCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGa                                                                                                                         <  2:621985/140‑1 (MQ=255)                  tCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCAc                                                                                                                                        >  2:510835/1‑123 (MQ=255)                  tCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATg                                                                                                                       <  1:365683/140‑1 (MQ=255)                  tCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATg                                                                                                                       <  1:866820/140‑1 (MQ=255)                  tCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATg                                                                                                                       >  2:375509/1‑140 (MQ=255)                  tCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATg                                                                                                                       >  2:338335/1‑140 (MQ=255)                  tCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATg                                                                                                                       >  2:35295/1‑140 (MQ=255)                  tCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATg                                                                                                                       >  2:947368/1‑140 (MQ=255)                  tCGCTGGTCGAGGCTTTCACACCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATg                                                                                                                       <  2:931385/140‑1 (MQ=255)                    gCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCa                                                                                                                     >  1:982555/1‑140 (MQ=255)                     cTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCAc                                                                                                                    <  1:35295/140‑1 (MQ=255)                      tGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACt                                                                                                                   <  1:252899/140‑1 (MQ=255)                      tGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAACAACGCCCAGCGGCACAGTTCCATATCCTGATGCACt                                                                                                                   <  1:505857/140‑1 (MQ=255)                          cGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCa                                                                                                               <  1:92836/140‑1 (MQ=255)                                       ccGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTcg                                                                                                  <  1:400056/140‑1 (MQ=255)                                            aaTGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCAt                                                                                             >  2:794197/1‑140 (MQ=255)                                                  gACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCg                                                                                       <  2:898245/140‑1 (MQ=255)                                                    cATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCgag                                                                                     >  2:304844/1‑140 (MQ=255)                                                       tGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGc                                                                                  >  1:956030/1‑140 (MQ=255)                                                       tGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGc                                                                                  >  2:458315/1‑140 (MQ=255)                                                         ccTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCAc                                                                                >  2:163783/1‑140 (MQ=255)                                                         ccTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCAc                                                                                >  2:30878/1‑140 (MQ=255)                                                           tttCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCa                                                                              <  1:669185/140‑1 (MQ=255)                                                           tttCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCa                                                                              <  1:1029440/140‑1 (MQ=255)                                                            ttCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAg                                                                             >  2:454427/1‑140 (MQ=255)                                                             tCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGc                                                                            >  2:131079/1‑140 (MQ=255)                                                                ccaccTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGGGAGAGCACCAGCTGt                                                                         <  2:717792/140‑1 (MQ=255)                                                                   ccTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTg                                                                      <  1:794197/140‑1 (MQ=255)                                                                    cTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCTACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGc                                                                     >  1:402101/1‑140 (MQ=255)                                                                     tGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGcc                                                                    <  1:338335/140‑1 (MQ=255)                                                                     tGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGcc                                                                    <  1:304844/140‑1 (MQ=255)                                                                     tGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGcc                                                                    <  1:903212/140‑1 (MQ=255)                                                                     tGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGcc                                                                    <  1:454427/140‑1 (MQ=255)                                                                     tGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGcc                                                                    <  2:541653/140‑1 (MQ=255)                                                                      ggATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGccc                                                                   <  2:858127/140‑1 (MQ=255)                                                                       gATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCa                                                                              <  1:809244/128‑1 (MQ=255)                                                                       gATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCa                                                                              >  2:809244/1‑128 (MQ=255)                                                                        aTCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTg                                                                 >  1:920850/1‑140 (MQ=255)                                                                             gACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGt                                                                         >  2:760014/1‑127 (MQ=255)                                                                             gACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGt                                                                         <  1:760014/127‑1 (MQ=255)                                                                             gACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTg                                                                 <  2:187473/135‑1 (MQ=255)                                                                             gACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTg                                                                 >  1:187473/1‑135 (MQ=255)                                                                             gACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACgg                                                            >  2:514695/1‑140 (MQ=255)                                                                              aCGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGc                                                           >  2:899179/1‑140 (MQ=255)                                                                                    cgTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGaa                                                     >  1:603476/1‑140 (MQ=255)                                                                                      ttCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGc                                                   >  2:1037650/1‑140 (MQ=255)                                                                                        cAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGa                                                 <  2:956030/140‑1 (MQ=255)                                                                                          gCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACg                                               <  1:30878/140‑1 (MQ=255)                                                                                          gCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACg                                               <  1:479397/140‑1 (MQ=255)                                                                                                aTCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTc                                         >  1:280866/1‑140 (MQ=255)                                                                                                        cACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAAcgcg                                 >  2:1025654/1‑140 (MQ=255)                                                                                                        cACCGACTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAAcgcg                                 >  2:883612/1‑140 (MQ=255)                                                                                                          ccGACTGCTCATCTTTCAGCAACGTCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACgcggc                               >  1:1084752/1‑140 (MQ=255)                                                                                                           ctaCTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACgcggcg                              <  2:402101/138‑1 (MQ=255)                                                                                                             aCTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTa                            >  1:923174/1‑140 (MQ=255)                                                                                                             aCTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTa                            >  1:499453/1‑140 (MQ=255)                                                                                                             aCTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTa                            >  1:699713/1‑140 (MQ=255)                                                                                                             aCTGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTa                            >  1:726187/1‑140 (MQ=255)                                                                                                               tGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAAt                          >  2:220761/1‑140 (MQ=255)                                                                                                               tGCTCATCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAAt                          >  1:710905/1‑140 (MQ=255)                                                                                                                     tCTTTCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGtt                    <  2:228798/140‑1 (MQ=255)                                                                                                                         tCAGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTttct                >  2:64607/1‑140 (MQ=255)                                                                                                                           aGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCGTAACGCGGCGTAATACCGTTttcttc              >  1:758745/1‑140 (MQ=255)                                                                                                                           aGCAACGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTttcttc              >  1:508282/1‑140 (MQ=255)                                                                                                                               aCGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATa                         <  1:942663/125‑1 (MQ=255)                                                                                                                               aCGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATa                         >  2:942663/1‑125 (MQ=255)                                                                                                                               aCGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTTTCTTCAGGc          <  1:947368/140‑1 (MQ=255)                                                                                                                                cgtccAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTTTCTTCAGGCa         <  2:1084752/137‑1 (MQ=255)                                                                                                                                cGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGtt                    >  1:813026/1‑129 (MQ=255)                                                                                                                                cGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGtt                    <  2:813026/129‑1 (MQ=255)                                                                                                                                cGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTTTCTTCAGGCa         >  2:614305/1‑140 (MQ=255)                                                                                                                                cGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTTTCTTCAGGCa         <  1:883612/140‑1 (MQ=255)                                                                                                                                cGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTTTCTTCAGGCa         <  1:458315/140‑1 (MQ=255)                                                                                                                                cGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTTTCTTCAGGCa         <  2:1048721/140‑1 (MQ=255)                                                                                                                                cGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTTTCTTCAGGCa         >  1:122258/1‑140 (MQ=255)                                                                                                                                cGCCCAGCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACACTGTTCATAACGCGGCGTAATACCGTTTTCTTCAGGCa         >  2:119370/1‑140 (MQ=255)                                                                                                                                      gCGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTTTCTTCAGGCATGATcc   <  1:375509/140‑1 (MQ=255)                                                                                                                                       cGGCACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTTTCTTCAGGCATGATCCa  <  1:1037650/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CCCAGATTGCCAGACCATCGCTGGTCGAGGCTTTCACGCCGTTAATGGTGACATTGCCTTTCACCACCTGGATCCAGACGCGGCGTTCAGCGGCAATCTGATGCACCGACTGCTCATCTTTCAGCAACGCCCAGCGGTACAGTTCCATATCCTGATGCACTTTCAATGAACCATCTCGCGCATCCGGCGAGAGCACCAGCTGTTTGCCCTGTACGGCATCGAAGCGACGCTGTTCATAACGCGGCGTAATACCGTTTTCTTCAGGCATGATCCA  >  NC_000913/3579021‑3579294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑