Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,729,740	T→C	V342V (GTA→GTG)	xylA ←	xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,729,740	0	T	C	98.6%	239.9 / ‑3.3	70	V342V (GTA→GTG)	xylA	xylose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (35/34);  minor base G (0/1);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

ACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTG  >  NC_000913/3729608‑3729875                                                                                                                                     |                                                                                                                                        aCGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTgg                                                                                                                                  >  1:689005/1‑140 (MQ=255)       cgcgATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGa                                                                                                                            >  2:241189/1‑140 (MQ=255)       cgcgATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGa                                                                                                                            >  1:471911/1‑140 (MQ=255)                 ttATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGacc                                                                                                                  >  1:572613/1‑140 (MQ=255)                  tATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGacca                                                                                                                 <  2:554281/140‑1 (MQ=255)                    tCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGaccacc                                                                                                               <  2:716767/140‑1 (MQ=255)                        gCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCggtg                                                                                                           >  2:521237/1‑140 (MQ=255)                            gCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGAGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGa                                                                                                       >  1:9136/1‑140 (MQ=255)                             ccATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGGCCGTAAAACAGATCATATTTATCAGTACTTTGACGACGGACTTTGGCATCGAAGTTCAGACCACCGGTGGTGaa                                                                                                      <  2:783314/140‑1 (MQ=255)                                    cAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCt                                                                                               >  1:264403/1‑140 (MQ=255)                                          tGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTg                                                                                         >  2:688474/1‑140 (MQ=255)                                          tGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTg                                                                                         >  1:466691/1‑140 (MQ=255)                                          tGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTg                                                                                         >  1:541651/1‑140 (MQ=255)                                           gcgcgcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTga                                                                                        <  1:688474/140‑1 (MQ=255)                                           gcgcgcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTga                                                                                        <  1:820485/140‑1 (MQ=255)                                           gcgcgcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTga                                                                                        <  1:884106/140‑1 (MQ=255)                                            cgcgcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTgag                                                                                       <  1:810237/140‑1 (MQ=255)                                            cgcgcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTgag                                                                                       <  1:521237/140‑1 (MQ=255)                                            cgcgcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTgag                                                                                       >  2:305326/1‑140 (MQ=255)                                            cgcgcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTgag                                                                                       >  2:599470/1‑140 (MQ=255)                                            cgcgcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTga                                                                                        <  2:148422/139‑1 (MQ=255)                                            cgcgcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTga                                                                                        <  1:993484/139‑1 (MQ=255)                                            cgcgcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTga                                                                                        >  2:570341/1‑139 (MQ=255)                                            cgcgcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTga                                                                                        >  2:993484/1‑139 (MQ=255)                                            cgcgcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTga                                                                                        >  1:148422/1‑139 (MQ=255)                                            cgcgcTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTga                                                                                        <  1:570341/139‑1 (MQ=255)                                                 tGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAAttt                                                                                  <  1:705406/140‑1 (MQ=255)                                                 tGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAAttt                                                                                  >  1:776842/1‑140 (MQ=255)                                                 tGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAAttt                                                                                  >  1:265417/1‑140 (MQ=255)                                                 tGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAAttt                                                                                  <  2:471882/140‑1 (MQ=255)                                                           aGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCAc                                                                        >  1:142273/1‑140 (MQ=255)                                                            gcgcCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCAcc                                                                       <  2:604509/140‑1 (MQ=255)                                                             cgcCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCAcc                                                                       >  2:169729/1‑139 (MQ=255)                                                             cgcCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCAcc                                                                       <  1:169729/139‑1 (MQ=255)                                                             cgcCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCa                                                                      >  1:189428/1‑140 (MQ=255)                                                                  gTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCa                                                                 <  2:142273/140‑1 (MQ=255)                                                                  gTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCa                                                                 <  2:173900/140‑1 (MQ=255)                                                                  gTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCa                                                                 <  2:256572/140‑1 (MQ=255)                                                                    gCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCAtt                                                               <  2:686074/140‑1 (MQ=255)                                                                    gCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCAtt                                                               <  1:305326/140‑1 (MQ=255)                                                                    gCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCAtt                                                               <  2:541651/140‑1 (MQ=255)                                                                    gCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCAtt                                                               <  2:466691/140‑1 (MQ=255)                                                                    gCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCAtt                                                               <  2:689005/140‑1 (MQ=255)                                                                          gTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTc                                                         >  1:548405/1‑140 (MQ=255)                                                                            aTCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCca                                                       <  2:548405/140‑1 (MQ=255)                                                                              ccATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCATTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCcaca                                                     <  2:189428/140‑1 (MQ=255)                                                                               cATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCacac                                                    <  1:843416/140‑1 (MQ=255)                                                                                  cgcgCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGt                                                 >  1:291414/1‑140 (MQ=255)                                                                                  cgcgCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGt                                                 >  1:691007/1‑140 (MQ=255)                                                                                    cgcCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTc                                               <  2:265417/140‑1 (MQ=255)                                                                                    cgcCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTc                                               >  1:556078/1‑140 (MQ=255)                                                                                    cgcCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTc                                               >  1:397005/1‑140 (MQ=255)                                                                                                cGTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCg                                   <  1:599470/140‑1 (MQ=255)                                                                                                 gTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCgg                                  <  2:397005/140‑1 (MQ=255)                                                                                                 gTAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGGGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCgg                                  <  2:346770/140‑1 (MQ=255)                                                                                                  tAAAACAGATCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCggg                                 >  2:880868/1‑139 (MQ=255)                                                                                                          aTCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTcccagc                         <  2:291414/140‑1 (MQ=255)                                                                                                          aTCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTcccagc                         >  1:727780/1‑140 (MQ=255)                                                                                                           tCATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTcccagcc                        <  2:556078/140‑1 (MQ=255)                                                                                                            cATATTTATCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTcccagccc                       >  1:604354/1‑140 (MQ=255)                                                                                                                    tCAGTACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTgcgc               >  2:663220/1‑140 (MQ=255)                                                                                                                        tACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGt                    <  2:86654/131‑1 (MQ=255)                                                                                                                        tACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGt                    >  1:86654/1‑131 (MQ=255)                                                                                                                        tACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCg           <  1:663220/140‑1 (MQ=255)                                                                                                                        tACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCg           >  2:715918/1‑140 (MQ=255)                                                                                                                        tACTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCg           >  2:32836/1‑140 (MQ=255)                                                                                                                          cTTTGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGcc         >  1:105813/1‑140 (MQ=255)                                                                                                                             tGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACg      >  2:362655/1‑140 (MQ=255)                                                                                                                             tGACGACGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGCTGTCCCAGCCCAGTTGCGCATCGCCACg      >  1:612308/1‑140 (MQ=255)                                                                                                                              gacgacGCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACgg     <  1:959444/140‑1 (MQ=255)                                                                                                                                 gacgCACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTg  <  2:572613/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        ACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTG  >  NC_000913/3729608‑3729875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑