Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	358,871	C→T	Q25* (CAG→TAG)	cynT →	carbonic anhydrase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	358,871	0	C	T	100.0%	270.9 / NA	76	Q25* (CAG→TAG)	cynT	carbonic anhydrase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/35);  total (41/35)

ACTTTTACCTTATGACAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAACAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCGGGCAATATCG  >  NC_000913/358734‑358997                                                                                                                                          |                                                                                                                               aCTTTTACCTTATGACAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAg                                                                                                                              >  2:24691/1‑140 (MQ=255)   ttttACCTTATGACAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCt                                                                                                                            <  1:708702/140‑1 (MQ=255)   ttttACCTTATGACAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCt                                                                                                                            <  2:727157/140‑1 (MQ=255)     ttACCTTATGACAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTgg                                                                                                                          <  1:24691/140‑1 (MQ=255)         cTTATGACAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGac                                                                                                                      <  1:748446/140‑1 (MQ=255)                   tCGGCGAGTAGTCTGCCTCTTATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGac                                                                                                                      >  1:1016765/1‑130 (MQ=255)                   tCGGCGAGTAGTCTGCCTCTTATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGac                                                                                                                      <  2:1016765/130‑1 (MQ=255)                   tCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGc                                                                                                            >  1:962928/1‑140 (MQ=255)                          gtagtCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGca                                                                                                     <  2:255946/140‑1 (MQ=255)                          gtagtCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGca                                                                                                     <  2:962928/140‑1 (MQ=255)                           tagtCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGcac                                                                                                    >  1:343459/1‑140 (MQ=255)                           tagtCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGcac                                                                                                    <  2:525828/140‑1 (MQ=255)                                tGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACttt                                                                                               >  1:681334/1‑140 (MQ=255)                                  ccTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACttttt                                                                                             <  2:529232/140‑1 (MQ=255)                                           ccAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGc                                                                                    <  2:681334/140‑1 (MQ=255)                                            cAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACttttt                                                                                             >  2:616062/1‑130 (MQ=255)                                            cAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACttttt                                                                                             <  1:616062/130‑1 (MQ=255)                                              gagaCAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCGAAGCCCGCGCACACTTTTTATCTCCTGCTcc                                                                                 >  2:157997/1‑140 (MQ=255)                                               agacagacAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCg                                                                                >  1:525057/1‑140 (MQ=255)                                                gacagacaGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGa                                                                               <  1:849845/140‑1 (MQ=255)                                                   agacagaGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAg                                                                            <  2:525057/140‑1 (MQ=255)                                                        gagGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTc                                                                       <  2:383543/140‑1 (MQ=255)                                                         agGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCt                                                                      <  2:114901/140‑1 (MQ=255)                                                           gTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTgg                                                                    >  2:1028165/1‑140 (MQ=255)                                                              aaCGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTcc                                                                 >  1:536289/1‑140 (MQ=255)                                                              aaCGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTG‑‑GACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCt                                                               >  2:596354/1‑140 (MQ=255)                                                               aCGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTccc                                                                >  2:1050156/1‑140 (MQ=255)                                                                 ggTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTg                                                              >  2:43059/1‑140 (MQ=255)                                                                 ggTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTg                                                              >  1:1029670/1‑140 (MQ=255)                                                                 ggTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTg                                                              >  2:706677/1‑140 (MQ=255)                                                                   tGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAg                                                            >  2:580298/1‑140 (MQ=255)                                                                     aaaGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCt                                                          >  2:362410/1‑140 (MQ=255)                                                                     aaaGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCt                                                          >  2:624970/1‑140 (MQ=255)                                                                     aaaGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCt                                                          >  1:538030/1‑140 (MQ=255)                                                                          gaTTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGa                                                             >  2:883192/1‑132 (MQ=255)                                                                          gaTTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGa                                                             <  1:883192/132‑1 (MQ=255)                                                                          gaTTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGa                                                     <  1:1050156/140‑1 (MQ=255)                                                                           attattGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGAc                                                    >  2:276399/1‑140 (MQ=255)                                                                           attattGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGAc                                                    <  2:536289/140‑1 (MQ=255)                                                                           attattGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGAc                                                    >  1:668512/1‑140 (MQ=255)                                                                               ttGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGt                                                       <  1:1069315/133‑1 (MQ=255)                                                                               ttGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGt                                                       >  2:1069315/1‑133 (MQ=255)                                                                                 gATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTG‑‑GACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTg                                            <  1:596354/140‑1 (MQ=255)                                                                                     gATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAg                                          >  1:860568/1‑140 (MQ=255)                                                                                       ttCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGcc                                        >  2:534376/1‑140 (MQ=255)                                                                                       ttCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGcc                                        >  1:984281/1‑140 (MQ=255)                                                                                       ttCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGcc                                        >  1:725935/1‑140 (MQ=255)                                                                                       ttCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGcc                                        >  1:914369/1‑140 (MQ=255)                                                                                         ccTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTg                                      <  2:185797/140‑1 (MQ=255)                                                                                          cTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTgg                                     <  2:860568/140‑1 (MQ=255)                                                                                          cTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTgg                                     >  1:195083/1‑140 (MQ=255)                                                                                              aaTTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGAt                                 <  2:343459/140‑1 (MQ=255)                                                                                               aTTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATc                                >  1:1021261/1‑140 (MQ=255)                                                                                                    aGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGc                                         >  1:790427/1‑126 (MQ=255)                                                                                                    aGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGc                                         <  2:790427/126‑1 (MQ=255)                                                                                                    aGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTc                           >  2:514557/1‑140 (MQ=255)                                                                                                    aGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTc                           >  1:470896/1‑140 (MQ=255)                                                                                                     gcgcGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGAt                                 <  2:232677/133‑1 (MQ=255)                                                                                                     gcgcGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGAt                                 >  1:232677/1‑133 (MQ=255)                                                                                                      cgcgAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACCTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGt                         >  2:298737/1‑140 (MQ=255)                                                                                                            gCATTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCg                   <  2:1021261/140‑1 (MQ=255)                                                                                                              aTTTCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCa                 <  1:218872/140‑1 (MQ=255)                                                                                                                ttCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAAc               <  1:534376/140‑1 (MQ=255)                                                                                                                ttCCGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAAc               <  1:276399/140‑1 (MQ=255)                                                                                                                  ccGAAGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAAcgc             <  2:914369/140‑1 (MQ=255)                                                                                                                     aaGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCggg          >  2:254527/1‑140 (MQ=255)                                                                                                                     aaGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCggg          <  1:706677/140‑1 (MQ=255)                                                                                                                     aaGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCggg          >  2:751953/1‑140 (MQ=255)                                                                                                                      aGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCggg          <  1:1023712/139‑1 (MQ=255)                                                                                                                      aGCGGGAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCggg          >  2:1023712/1‑139 (MQ=255)                                                                                                                          ggAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCGGGCAata     >  1:324130/1‑140 (MQ=255)                                                                                                                          ggAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCGGGCAat      <  1:401128/139‑1 (MQ=255)                                                                                                                          ggAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCGGGCAat      >  2:401128/1‑139 (MQ=255)                                                                                                                          ggAAGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCACCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCGGGCAata     >  2:267303/1‑140 (MQ=255)                                                                                                                            aaGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCGGGCAATATc   <  1:254527/140‑1 (MQ=255)                                                                                                                             aGCCTTGTTTAAATAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCGGGCAATATCg  <  1:43059/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                               ACTTTTACCTTATGACAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAACAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATTCGCAACGCGGGCAATATCG  >  NC_000913/358734‑358997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑