Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,885,773	A→G	Y233C (TAC→TGC)	yidC →	membrane protein insertase YidC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,885,773	0	A	G	100.0%	184.8 / NA	51	Y233C (TAC→TGC)	yidC	membrane protein insertase YidC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/27);  total (24/27)

GCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCG  >  NC_000913/3885636‑3885904                                                                                                                                          |                                                                                                                                    gCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCACGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCa                                                                                                                                   >  2:993255/1‑140 (MQ=255)   tGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAg                                                                                                                                 >  2:783621/1‑140 (MQ=255)             ctcGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGtt                                                                                                                               <  2:22694/132‑1 (MQ=255)             ctcGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGtt                                                                                                                               >  1:22694/1‑132 (MQ=255)                   tGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCg                                                                                                                 >  2:535297/1‑140 (MQ=255)                    ggTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGa                                                                                                                <  1:931548/140‑1 (MQ=255)                         gTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACg                                                                                                           <  2:334605/140‑1 (MQ=255)                         gTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACg                                                                                                           <  1:535297/140‑1 (MQ=255)                          ttGAAGCAATCCATCACTCTGCCACCGCATCTCGATACTGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGa                                                                                                          >  2:688315/1‑140 (MQ=255)                           tGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGaa                                                                                                         <  1:207004/140‑1 (MQ=255)                           tGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGaa                                                                                                         <  1:377014/140‑1 (MQ=255)                                  aTCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGa                                                                                                  >  2:455684/1‑140 (MQ=255)                                    ccATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAAc                                                                                                <  2:1087455/140‑1 (MQ=255)                                       tCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACAtc                                                                                             <  1:224181/140‑1 (MQ=255)                                       tCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTACACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACAtc                                                                                             <  1:239724/140‑1 (MQ=255)                                        cACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACAtct                                                                                            >  1:571659/1‑140 (MQ=255)                                             tGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTg                                                                                                   >  1:38475/1‑128 (MQ=255)                                             tGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTg                                                                                                   <  2:38475/128‑1 (MQ=255)                                             tGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCg                                                                                       <  1:783621/140‑1 (MQ=255)                                               ccACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGaa                                                                                     <  2:571659/140‑1 (MQ=255)                                               ccACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGaa                                                                                     >  1:219235/1‑140 (MQ=255)                                               ccACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGaa                                                                                     <  1:2789/140‑1 (MQ=255)                                                     cATCTCGATACTGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAggtgg                                                                               <  1:688315/140‑1 (MQ=255)                                                     cATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAggtgg                                                                               >  1:912705/1‑140 (MQ=255)                                                          cGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCACGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTggg                                                                          <  1:993255/140‑1 (MQ=255)                                                              aCCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGc                                                                      <  2:98577/140‑1 (MQ=255)                                                              aCCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGc                                                                      <  2:509398/140‑1 (MQ=255)                                                                cGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGa                                                                    >  1:874693/1‑140 (MQ=255)                                                                   aaGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGAtgc                                                                 <  2:105333/140‑1 (MQ=255)                                                                     gcagcaACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGAtgctg                                                               <  2:352184/140‑1 (MQ=255)                                                                      cagcaACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGAtgctgc                                                              <  2:219235/140‑1 (MQ=255)                                                                         caACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAAc                                                           >  1:65317/1‑140 (MQ=255)                                                                               cGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTAtt                                                     >  1:807364/1‑140 (MQ=255)                                                                                 cACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTc                                                   >  1:514994/1‑140 (MQ=255)                                                                                    tGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTcgcg                                                <  2:874693/140‑1 (MQ=255)                                                                                     gCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGa                                               <  2:807364/140‑1 (MQ=255)                                                                                      cacaCCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGAc                                              >  2:702967/1‑140 (MQ=255)                                                                                           cTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGt                                         <  2:583066/140‑1 (MQ=255)                                                                                               cGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGAt                                     <  1:455684/140‑1 (MQ=255)                                                                                                     gcggcgTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCa                               >  1:968083/1‑140 (MQ=255)                                                                                                     gcggcgTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCa                               >  1:968989/1‑140 (MQ=255)                                                                                                       ggcgTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATa                             >  1:71481/1‑140 (MQ=255)                                                                                                         cgTACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAac                           >  2:280394/1‑140 (MQ=255)                                                                                                           tACTCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAacga                         <  2:514994/140‑1 (MQ=255)                                                                                                              tCCACGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACgg                      <  2:968083/140‑1 (MQ=255)                                                                                                                  cGCCTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTAcc                  >  1:635023/1‑140 (MQ=255)                                                                                                                     cTGACGAGAAGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACcaac               >  2:545412/1‑140 (MQ=255)                                                                                                                             aaGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCta       <  2:968989/140‑1 (MQ=255)                                                                                                                             aaGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCta       >  2:187183/1‑140 (MQ=255)                                                                                                                              aGTATGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCtat      >  1:870359/1‑140 (MQ=255)                                                                                                                                  tGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCg  >  1:315372/1‑140 (MQ=255)                                                                                                                                  tGAGAAATGCAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCg  <  2:320243/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    GCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGACGAGAAGTATGAGAAATACAAGTTCGATACCATTGCCGATAACGAAAACCTGAACATCTCTTCGAAAGGTGGTTGGGTGGCGATGCTGCAACAGTATTTCGCGACGGCGTGGATCCCGCATAACGACGGTACCAACAACTTCTATACCG  >  NC_000913/3885636‑3885904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑