Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,147,556	A→G	R254R (CGT→CGC)	yijO ←	putative DNA‑binding transcriptional regulator YijO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,147,556	0	A	G	100.0%	197.1 / NA	55	R254R (CGT→CGC)	yijO	putative DNA‑binding transcriptional regulator YijO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/31);  total (24/31)

GAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCC  >  NC_000913/4147422‑4147689                                                                                                                                       |                                                                                                                                      gAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCa                                                                                                                                  <  2:1059066/140‑1 (MQ=255)  aGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAg                                                                                                                                 <  1:95754/140‑1 (MQ=255)   gcgcGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGa                                                                                                                                <  2:921033/140‑1 (MQ=255)     gcgGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAag                                                                                                                              >  1:103354/1‑140 (MQ=255)      cgGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAagt                                                                                                                             >  2:860428/1‑140 (MQ=255)                   gCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGAc                                                                                                                >  2:490969/1‑140 (MQ=255)                     tttCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACaa                                                                                                              <  1:1079706/140‑1 (MQ=255)                     tttCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACaa                                                                                                              <  1:517874/140‑1 (MQ=255)                          gcgcCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCg                                                                                                         >  1:414716/1‑140 (MQ=255)                            gccgcAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCa                                                                                                       >  1:854574/1‑140 (MQ=255)                              cgcAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAgg                                                                                                     >  1:261313/1‑140 (MQ=255)                                 agcaAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGt                                                                                                  <  1:971640/140‑1 (MQ=255)                                     aaCCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTgcgc                                                                                              <  2:414716/140‑1 (MQ=255)                                       cccATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCa                                                                                            <  2:261313/140‑1 (MQ=255)                                           tCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCActtc                                                                                        >  2:128783/1‑140 (MQ=255)                                              tATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCttt                                                                                     <  1:128783/140‑1 (MQ=255)                                                ttCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTa                                                                                   >  2:116332/1‑140 (MQ=255)                                                 tCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTAc                                                                                  <  2:854574/140‑1 (MQ=255)                                                  cTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGTGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTAcc                                                                                 >  2:985433/1‑140 (MQ=255)                                                    ggAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGTGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt                                                                               <  1:985433/140‑1 (MQ=255)                                                    ggAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt                                                                               <  2:424157/140‑1 (MQ=255)                                                     gAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCttt                                                                                     <  2:755008/133‑1 (MQ=255)                                                     gAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCttt                                                                                     >  1:755008/1‑133 (MQ=255)                                                     gAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTc                                                                              >  2:1059624/1‑140 (MQ=255)                                                      agtagtCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCa                                                                             >  1:705515/1‑140 (MQ=255)                                                      agtagtCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCa                                                                             <  2:916291/140‑1 (MQ=255)                                                        tagtCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAgg                                                                           >  2:107406/1‑140 (MQ=255)                                                        tagtCGGTTTTTCTGTCAGTTAGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTc                                                                              <  1:228892/137‑1 (MQ=255)                                                        tagtCGGTTTTTCTGTCAGTTAGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTc                                                                              >  2:228892/1‑137 (MQ=255)                                                          gtCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt                                                                               <  2:663501/134‑1 (MQ=255)                                                          gtCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt                                                                               >  1:663501/1‑134 (MQ=255)                                                          gtCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTc                                                                         >  2:430107/1‑140 (MQ=255)                                                            cGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCAt                                                                       <  1:430107/140‑1 (MQ=255)                                                            cGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCAt                                                                       <  1:107406/140‑1 (MQ=255)                                                               tttttCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGc                                                                    <  2:553090/140‑1 (MQ=255)                                                               tttttCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGc                                                                    >  1:614298/1‑140 (MQ=255)                                                                 tttCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCt                                                                  <  2:705515/140‑1 (MQ=255)                                                                     tGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCa                                                              >  1:200457/1‑140 (MQ=255)                                                                        cAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATa                                                           <  1:1059624/140‑1 (MQ=255)                                                                                        tGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCACCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTc                                           >  1:141408/1‑140 (MQ=255)                                                                                          ccGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATa                                                           >  1:973631/1‑122 (MQ=255)                                                                                          ccGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATa                                                           <  2:973631/122‑1 (MQ=255)                                                                                             gCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCg                                                <  1:826983/130‑1 (MQ=255)                                                                                             gCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCg                                                >  2:826983/1‑130 (MQ=255)                                                                                                atatTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTcgcg                                   <  2:1030574/140‑1 (MQ=255)                                                                                                  atTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCgtg                                 <  2:471307/140‑1 (MQ=255)                                                                                                  atTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCgtg                                 <  2:200457/140‑1 (MQ=255)                                                                                                       gACGGCGAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGAtt                            >  2:415104/1‑140 (MQ=255)                                                                                                             gAGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTa                      <  2:614298/140‑1 (MQ=255)                                                                                                              aGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTAt                     <  1:399588/140‑1 (MQ=255)                                                                                                              aGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTAGCGTGTGATTCAGGTAt                     <  2:630654/140‑1 (MQ=255)                                                                                                              aGCGTTCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTAGCGTGTGATTCAGGTAt                     >  1:630654/1‑140 (MQ=255)                                                                                                                  ttCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGt                 >  1:937653/1‑140 (MQ=255)                                                                                                                   tCGGTGTTTTTACGAAACAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGtt                <  1:415104/140‑1 (MQ=255)                                                                                                                                 aaaCAGGCGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGccc  <  1:116332/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      GAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGGCCC  >  NC_000913/4147422‑4147689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑