Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 375,928 C→T T157I (ACA→ATA)  mhpT → 3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913375,9280CT98.2% 191.8 / NA 57T157I (ACA→ATA) mhpT3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (31/25);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGACCGGTGTCGGGCTGGGGGCGGCGTTGCCGAATCTTATCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGACACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTCGGCGGTTTTCGCTGGCGAAAAACAGTC  >  NC_000913/375792‑376060
                                                                                                                                        |                                                                                                                                    
atgaCCGGTGTCGGGCTGGGGGCGGCGTTGCCGAATCTTATCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACt                                                                                                                                   >  2:279323/1‑140 (MQ=255)
       gtgtCGGGCTGGGGGCGGCGTTGCCGAATCTTATCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTc                                                                                                                            >  2:500938/1‑140 (MQ=255)
          tCGGGCTGGGGGCGGCGTTGCCGAATCTTATCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTcgcg                                                                                                                         <  1:711540/140‑1 (MQ=255)
          tCGGGCTGGGGGCGGCGTTGCCGAATCTTATCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTcgcg                                                                                                                         >  1:4417/1‑140 (MQ=255)
             ggCTGGGGGCGGCGTTGCCGAATCTTATCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCgggg                                                                                                                      <  1:275181/140‑1 (MQ=255)
                  ggggCGGCGTTGCCGAATCTTATCGCCCTGCCGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGACACTGGGTTTCGCGGGGGCaaa                                                                                                                 >  2:840651/1‑140 (MQ=255)
                  ggggCGGCGTTGCCGAATCTTATCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCaaa                                                                                                                 >  2:399884/1‑140 (MQ=255)
                  ggggCGGCGTTGCCGAATCTTATCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCaaa                                                                                                                 >  2:286277/1‑140 (MQ=255)
                        gcgTTGCCGAATCTTATCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGc                                                                                                           <  1:286277/140‑1 (MQ=255)
                              ccGAATCTTATCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCa                                                                                                     >  2:90210/1‑140 (MQ=255)
                                gAATCTTATCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCaaa                                                                                                   <  1:90210/140‑1 (MQ=255)
                                 aaTCTTATCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAAc                                                                                                  <  1:750278/140‑1 (MQ=255)
                                  aTCTTATCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACg                                                                                                 <  1:840651/140‑1 (MQ=255)
                                        tCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGgt                                                                                               >  2:692505/1‑136 (MQ=255)
                                        tCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGgt                                                                                               <  1:692505/136‑1 (MQ=255)
                                        tCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGttt                                                                                           >  2:340588/1‑140 (MQ=255)
                                        tCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGttt                                                                                           >  2:785144/1‑140 (MQ=255)
                                          gCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTg                                                                                         <  1:279323/140‑1 (MQ=255)
                                           cccTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTgg                                                                                        <  1:610852/140‑1 (MQ=255)
                                            ccTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTggg                                                                                       <  1:340588/140‑1 (MQ=255)
                                             cTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGt                                                                                      >  2:571154/1‑140 (MQ=255)
                                              tGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTa                                                                                     >  1:1087543/1‑140 (MQ=255)
                                               gACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAg                                                                                    >  2:741375/1‑140 (MQ=255)
                                                  gTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAggtg                                                                                 <  1:297598/140‑1 (MQ=255)
                                                  gTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAggtg                                                                                 <  1:312728/140‑1 (MQ=255)
                                                        aGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGgtggtgtgg                                                                           <  1:785144/140‑1 (MQ=255)
                                                            gcgGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGTTGGCGGCGATACTGGTTTTCGCGGGGGCGAACTTAGCATGGCAAACGGGGTTTTGGGTAGGGGGTGTGGTGcc                                                                       >  2:297598/1‑140 (MQ=255)
                                                            gcgGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGtggtgtggtg                                                                         <  2:443706/138‑1 (MQ=255)
                                                            gcgGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGtggtgtggtg                                                                         >  1:443706/1‑138 (MQ=255)
                                                                    aCGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTc                                                               <  1:978875/140‑1 (MQ=255)
                                                                      gTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTg                                                             <  1:741375/140‑1 (MQ=255)
                                                                      gTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTg                                                             <  1:702838/140‑1 (MQ=255)
                                                                             tGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGc                                                      <  2:351438/140‑1 (MQ=255)
                                                                                  cGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGc                                                         <  1:659650/132‑1 (MQ=255)
                                                                                  cGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGc                                                         >  2:659650/1‑132 (MQ=255)
                                                                                            ccTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCgctggc                                       >  1:481708/1‑140 (MQ=255)
                                                                                               gATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGc                                    >  2:352439/1‑140 (MQ=255)
                                                                                               gATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGAGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGc                                    >  2:1056392/1‑140 (MQ=255)
                                                                                                    aTTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAg                               >  2:361240/1‑140 (MQ=255)
                                                                                                     ttGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGt                              >  1:1026818/1‑140 (MQ=255)
                                                                                                     ttGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGt                              >  1:133622/1‑140 (MQ=255)
                                                                                                          gtgtTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTcggcg                         >  1:120096/1‑140 (MQ=255)
                                                                                                          gtgtTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTcggcg                         >  2:860917/1‑140 (MQ=255)
                                                                                                          gtgtTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTcggcg                         >  1:795198/1‑140 (MQ=255)
                                                                                                          gtgtTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTcggcg                         >  2:404471/1‑140 (MQ=255)
                                                                                                          gtgtTCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTcggcg                         >  1:783521/1‑140 (MQ=255)
                                                                                                             ttCCCATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTCGGCGGtt                      >  1:416940/1‑140 (MQ=255)
                                                                                                               cccATTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTCGGCGGtttt                    >  2:45053/1‑140 (MQ=255)
                                                                                                                  aTTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTCGGCGGtt                      >  1:174360/1‑135 (MQ=255)
                                                                                                                  aTTGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTCGGCGGtt                      <  2:174360/135‑1 (MQ=255)
                                                                                                                    tGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTCGGCGGTTTTCGCTg               <  2:4417/140‑1 (MQ=255)
                                                                                                                    tGGCGCGGCGCTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTCGGCGGTTTTCGCTg               <  1:81381/140‑1 (MQ=255)
                                                                                                                         cggcgcTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTCGGCGGTTTTCGCTGGCGaa          >  2:347960/1‑140 (MQ=255)
                                                                                                                          ggcgcTGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTCGGCGGTTTTCGCTGGCGaaa         >  1:216403/1‑140 (MQ=255)
                                                                                                                               tGGCGGCGATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTCGGCGGTTTTCGCTGGCGAAAAACAg    <  2:686595/140‑1 (MQ=255)
                                                                                                                                 gcggcgATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTCGGCGGTTTTCGCTGGCGAAAAACAGTc  <  2:481708/140‑1 (MQ=255)
                                                                                                                                 gcggcgATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTCGGCGGTTTTCGCTGGCGAAAAACAGTc  <  1:404471/140‑1 (MQ=255)
                                                                                                                                 gcggcgATACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTAGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTCGGCGGTTTTCGCTGGCGAAAAACAGTc  >  1:20346/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                    
ATGACCGGTGTCGGGCTGGGGGCGGCGTTGCCGAATCTTATCGCCCTGACGTCTGAAGCCGCGGGTCCACGTTTTCGTGGGACGGCAGTGAGCCTGATGTATTGCGGTGTTCCCATTGGCGCGGCGCTGGCGGCGACACTGGGTTTCGCGGGGGCAAACTTAGCATGGCAAACGGTGTTTTGGGTAGGTGGTGTGGTGCCGTTGATTCTGGTGCCGCTATTAATGCGCTGGCTGCCGGAGTCGGCGGTTTTCGCTGGCGAAAAACAGTC  >  NC_000913/375792‑376060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑