Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,148,884	T→C	D461G (GAC→GGC)	eptC ←	phosphoethanolamine transferase EptC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,148,884	0	T	C	100.0%	256.4 / NA	73	D461G (GAC→GGC)	eptC	phosphoethanolamine transferase EptC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/37);  total (36/37)

GATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAATCGTTATATGACTCCAGCTCTTCCGCGTTTAATCCCGGC  >  NC_000913/4148756‑4149021                                                                                                                                 |                                                                                                                                          gATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATaa                                                                                                                                >  1:861563/1‑140 (MQ=255)    gAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAAcc                                                                                                                             >  2:819771/1‑140 (MQ=255)     aGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCa                                                                                                                            >  2:1032763/1‑140 (MQ=255)     aGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCa                                                                                                                            >  1:472301/1‑140 (MQ=255)          cTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGaaa                                                                                                                       >  1:468894/1‑140 (MQ=255)            tGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAAcc                                                                                                                     >  1:993612/1‑140 (MQ=255)            tGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAAcc                                                                                                                     >  1:203786/1‑140 (MQ=255)            tGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAAcc                                                                                                                     >  2:564748/1‑140 (MQ=255)               cATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGtt                                                                                                                  <  2:993612/140‑1 (MQ=255)                       tGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTtg                                                                                                          <  1:638262/140‑1 (MQ=255)                              cacaGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCttt                                                                                                   >  1:171924/1‑140 (MQ=255)                               acaGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTGGTAAACCTCTTCGCCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTa                                                                                                  <  1:564748/140‑1 (MQ=255)                               acaGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTa                                                                                                  <  2:861563/140‑1 (MQ=255)                                      gAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCtt                                                                                           >  1:82329/1‑140 (MQ=255)                                                 tgtACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTg                                                                                >  2:885176/1‑140 (MQ=255)                                                 tgtACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTg                                                                                >  2:64540/1‑140 (MQ=255)                                                   tACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGc                                                                              <  2:399399/140‑1 (MQ=255)                                                   tACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGc                                                                              <  2:472301/140‑1 (MQ=255)                                                     cATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCaa                                                                            >  1:379576/1‑140 (MQ=255)                                                     cATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCaa                                                                            >  1:491093/1‑140 (MQ=255)                                                      atatGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAAc                                                                           >  1:93574/1‑140 (MQ=255)                                                        atGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACca                                                                         >  2:107551/1‑140 (MQ=255)                                                        atGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTT‑AAGTCTTTAATCAGGCTGGCAACcac                                                                        >  2:588046/1‑140 (MQ=255)                                                          gACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACcaca                                                                       >  1:628860/1‑140 (MQ=255)                                                          gACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACcaca                                                                       >  1:579046/1‑140 (MQ=255)                                                          gACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACcaca                                                                       >  2:674869/1‑140 (MQ=255)                                                           aCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACAt                                                                      <  2:479866/140‑1 (MQ=255)                                                           aCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACAt                                                                      <  1:885176/140‑1 (MQ=255)                                                           aCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACAt                                                                      <  1:994703/140‑1 (MQ=255)                                                           aCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACAt                                                                      <  1:819771/140‑1 (MQ=255)                                                           aCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACAt                                                                      >  2:495382/1‑140 (MQ=255)                                                           aCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACAt                                                                      >  1:271935/1‑140 (MQ=255)                                                           aCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACAt                                                                      <  1:64540/140‑1 (MQ=255)                                                           aCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACAt                                                                      <  1:605466/140‑1 (MQ=255)                                                             gcgTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGa                                                                    <  1:473667/140‑1 (MQ=255)                                                              cgTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGAt                                                                   <  2:379576/140‑1 (MQ=255)                                                              cgTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGAt                                                                   <  2:203786/140‑1 (MQ=255)                                                              cgTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGAt                                                                   <  2:491093/140‑1 (MQ=255)                                                              cgTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGAt                                                                   <  1:674869/140‑1 (MQ=255)                                                               gTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGa                                                                    <  1:815077/138‑1 (MQ=255)                                                               gTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGa                                                                    >  2:815077/1‑138 (MQ=255)                                                               gTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATc                                                                  >  2:102119/1‑140 (MQ=255)                                                               gTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATc                                                                  >  1:1033987/1‑140 (MQ=255)                                                               gTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATc                                                                  >  1:719818/1‑140 (MQ=255)                                                                tCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCaa                                                                            <  2:695532/129‑1 (MQ=255)                                                                tCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCaa                                                                            >  1:695532/1‑129 (MQ=255)                                                                tCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCg                                                                 <  2:82329/140‑1 (MQ=255)                                                                tCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCg                                                                 >  2:782513/1‑140 (MQ=255)                                                                tCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCg                                                                 >  1:930317/1‑140 (MQ=255)                                                                tCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGACCg                                                                 >  1:297208/1‑140 (MQ=255)                                                                     ttGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTtata                                                            <  1:1032763/140‑1 (MQ=255)                                                                          cTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCg                                                                 <  2:963722/130‑1 (MQ=255)                                                                          cTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCg                                                                 >  1:963722/1‑130 (MQ=255)                                                                             aTTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCg                                                                 >  2:619702/1‑127 (MQ=255)                                                                             aTTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCg                                                                 <  1:619702/127‑1 (MQ=255)                                                                               tACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCg                                                  <  2:98158/140‑1 (MQ=255)                                                                               tACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCg                                                  <  1:102119/140‑1 (MQ=255)                                                                                   ccccTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGt                                              >  1:362083/1‑140 (MQ=255)                                                                                    cccTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTc                                             >  2:345278/1‑140 (MQ=255)                                                                                      cTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCAt                                           <  2:468894/140‑1 (MQ=255)                                                                                       tGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATa                                          <  2:1033987/140‑1 (MQ=255)                                                                                         aGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAAt                                        <  2:271935/140‑1 (MQ=255)                                                                                         aGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAAt                                        <  2:362083/140‑1 (MQ=255)                                                                                         aGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAAt                                        <  1:107551/140‑1 (MQ=255)                                                                                           ttttGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAATCg                                      <  2:405333/140‑1 (MQ=255)                                                                                                 gcggcggcgTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAATCGTTatat                                <  2:579046/140‑1 (MQ=255)                                                                                                    gcggcgTGTCGTAAACCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAATCGTTATATGAc                             <  2:538006/140‑1 (MQ=255)                                                                                                                aaaCCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAATCGTTATATGACTCCAGCTCTTcc                 <  2:783056/140‑1 (MQ=255)                                                                                                                aaaCCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAATCGTTATATGACTCCAGCTCTTcc                 <  2:628860/140‑1 (MQ=255)                                                                                                                 aaCCTCTTCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAATCGTTATATGACTCCAGCTCTTCcg                <  1:782513/140‑1 (MQ=255)                                                                                                                       ttCACCGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAATCGTTATATGACTCCAGCTCTTCCGCGTTTa          >  1:46236/1‑140 (MQ=255)                                                                                                                            cGTGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAATCGTTATATGACTCCAGCTCTTCCGCGTTTAATccc     >  2:62203/1‑140 (MQ=255)                                                                                                                              tGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAATCGTTATATGACTCCAGCTCTTCCGCGTTTAATCCcgg   >  2:295439/1‑140 (MQ=255)                                                                                                                              tGGCCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTT‑AAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAATCGTTATATGACTCCAGCTCTTCCGCGTTTAATCCcggc  <  1:588046/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                          GATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAACCACATGATCGTTATACAGGTTAGCGTTGTCATAATCGTTATATGACTCCAGCTCTTCCGCGTTTAATCCCGGC  >  NC_000913/4148756‑4149021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑