Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,357,077	A→G	V514A (GTG→GCG)	cadA ←	lysine decarboxylase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,357,077	0	A	G	100.0%	328.3 / NA	90	V514A (GTG→GCG)	cadA	lysine decarboxylase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (48/42);  total (48/42)

CGTTTAAAGTCAGTCAGAGCACGCAGCAGGCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCACGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTC  >  NC_000913/4356945‑4357210                                                                                                                                     |                                                                                                                                      cGTTTAAAGTCAGTCAGAGCACGCAGCAGGCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCt                                                                                                                                <  1:198208/140‑1 (MQ=255)   tttAAAGTCAGTCAGAGCACGCAGCAGGCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTgg                                                                                                                              <  2:908266/140‑1 (MQ=255)    ttAAAGTCAGTCAGAGCACGCAGCAGGCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGc                                                                                                                             <  2:753785/140‑1 (MQ=255)       aaGTCAGTCAGAGCACGCAGCAGGCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCgg                                                                                                                          <  1:417526/140‑1 (MQ=255)       aaGTCAGTCAGAGCACGCAGCAGGCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCgg                                                                                                                          <  2:539285/140‑1 (MQ=255)            agtcagAGCACGCAGCAGGCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGTCGGAATAc                                                                                                                     >  1:456749/1‑140 (MQ=255)                   gCACGCAGCAGGCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTc                                                                                                              <  2:897616/140‑1 (MQ=255)                    cACGCAGCAGGCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCg                                                                                                             >  2:375563/1‑140 (MQ=255)                       gcagcaGGCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTc                                                                                                          >  2:669337/1‑140 (MQ=255)                       gcagcaGGCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTc                                                                                                          >  1:778684/1‑140 (MQ=255)                            agGCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGt                                                                                                     >  1:362521/1‑140 (MQ=255)                             ggCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTg                                                                                                    <  1:72559/140‑1 (MQ=255)                             ggCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTg                                                                                                    >  1:166480/1‑140 (MQ=255)                              gCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATggag                                                                                                    >  1:479948/1‑137 (MQ=255)                              gCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTg                                                                                                    <  2:479948/139‑1 (MQ=255)                              gCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTg                                                                                                    >  1:793343/1‑139 (MQ=255)                              gCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTg                                                                                                    <  2:793343/139‑1 (MQ=255)                                   gTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCt                                                                                              >  2:505791/1‑140 (MQ=255)                                   gTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCt                                                                                              <  2:527782/140‑1 (MQ=255)                                     gCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCttt                                                                                            >  1:1028330/1‑140 (MQ=255)                                     gCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCttt                                                                                            >  2:259628/1‑140 (MQ=255)                                     gCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCttt                                                                                            >  1:297084/1‑140 (MQ=255)                                        ttGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTc                                                                                         <  1:937455/140‑1 (MQ=255)                                        ttGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTc                                                                                         <  1:277860/140‑1 (MQ=255)                                        ttGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTc                                                                                         <  1:259628/140‑1 (MQ=255)                                            tCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATc                                                                                     >  1:312832/1‑140 (MQ=255)                                              ttATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCa                                                                                       <  1:963022/136‑1 (MQ=255)                                              ttATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCa                                                                                       >  2:963022/1‑136 (MQ=255)                                                ctCGATACCGATGCTGAACTGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGATCGTCGAGGCATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCg                                                                                 >  2:765273/2‑140 (MQ=255)                                                aTCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCg                                                                                 >  2:752834/1‑140 (MQ=255)                                                     tACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTc                                                                            >  2:572239/1‑140 (MQ=255)                                                     tACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTc                                                                            >  1:916630/1‑140 (MQ=255)                                                     tACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTc                                                                            <  2:131065/140‑1 (MQ=255)                                                     tACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTGCCATCCCCGGAGTc                                                                            >  2:666534/1‑140 (MQ=255)                                                       ccGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTcag                                                                          <  2:166480/140‑1 (MQ=255)                                                          aTGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTcagcag                                                                       >  2:164838/1‑140 (MQ=255)                                                          aTGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTcagcag                                                                       >  2:304726/1‑140 (MQ=255)                                                          aTGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTcagcag                                                                       >  2:354458/1‑140 (MQ=255)                                                          aTGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTcagcag                                                                       >  2:390117/1‑140 (MQ=255)                                                          aTGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTcagcag                                                                       >  2:1011589/1‑140 (MQ=255)                                                          aTGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTcagcag                                                                       >  2:71202/1‑140 (MQ=255)                                                          aTGCTGAACAGGAACAGCAGGCTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTcagcag                                                                       >  2:430810/1‑140 (MQ=255)                                                           tGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAgg                                                                      <  1:752834/140‑1 (MQ=255)                                                           tGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAgg                                                                      <  1:54886/140‑1 (MQ=255)                                                           tGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAgg                                                                      >  1:545747/1‑140 (MQ=255)                                                           tGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAgg                                                                      >  2:173910/1‑140 (MQ=255)                                                               gaacaggaacagCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCAGGAGTCAGCAGGGTGa                                                                  >  1:125778/1‑140 (MQ=255)                                                                      aacagCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGAt                                                           <  1:505791/140‑1 (MQ=255)                                                                      aacagCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGAt                                                           <  1:521032/140‑1 (MQ=255)                                                                      aacagCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGAt                                                           <  2:545747/140‑1 (MQ=255)                                                                      aacagCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGAt                                                           <  2:916630/140‑1 (MQ=255)                                                                      aacagCAGGTCATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGAt                                                           <  1:1011589/140‑1 (MQ=255)                                                                         agcagGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCgg                                                        <  1:881044/140‑1 (MQ=255)                                                                          gcagGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCggg                                                       >  1:807509/1‑140 (MQ=255)                                                                           cagGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGt                                                      <  1:164838/140‑1 (MQ=255)                                                                           cagGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGt                                                      <  1:542171/140‑1 (MQ=255)                                                                           cagGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGt                                                      <  1:375563/140‑1 (MQ=255)                                                                               ttATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAg                                                  >  2:21953/1‑140 (MQ=255)                                                                               ttATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAg                                                  >  2:85353/1‑140 (MQ=255)                                                                                   aCGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGAt                                                           >  1:667535/1‑127 (MQ=255)                                                                                   aCGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGAt                                                           <  2:667535/127‑1 (MQ=255)                                                                                       aCCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACAtgt                                          >  1:842028/1‑140 (MQ=255)                                                                                        ccGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGt                                                      <  2:199698/127‑1 (MQ=255)                                                                                        ccGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGt                                                      >  1:199698/1‑127 (MQ=255)                                                                                        ccGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACAtgtg                                         >  2:778480/1‑140 (MQ=255)                                                                                        ccGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACAtgtg                                         <  1:217992/140‑1 (MQ=255)                                                                                         cGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGt                                                      <  1:475685/126‑1 (MQ=255)                                                                                         cGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGt                                                      >  2:475685/1‑126 (MQ=255)                                                                                         cGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGc                                        >  1:529880/1‑140 (MQ=255)                                                                                          ggTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCt                                       >  1:310019/1‑140 (MQ=255)                                                                                                 tcAACAACGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATc                                >  2:817184/1‑140 (MQ=255)                                                                                                    acaacGATGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGAt                             <  1:71202/140‑1 (MQ=255)                                                                                                           tGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTg                      <  2:1064927/140‑1 (MQ=255)                                                                                                           tGCCATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTg                      >  1:335140/1‑140 (MQ=255)                                                                                                              cATGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAg                   <  2:473017/140‑1 (MQ=255)                                                                                                                tGTTCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGcc                 >  1:943815/1‑140 (MQ=255)                                                                                                                  ttCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGaa                    >  1:1030146/1‑135 (MQ=255)                                                                                                                  ttCGTCGAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGaa                    <  2:1030146/135‑1 (MQ=255)                                                                                                                    cgtcgAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGc             <  2:297084/140‑1 (MQ=255)                                                                                                                    cgtcgAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGc             <  1:778480/140‑1 (MQ=255)                                                                                                                    cgtcgAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGc             <  1:173910/140‑1 (MQ=255)                                                                                                                    cgtcgAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGc             <  2:1028330/140‑1 (MQ=255)                                                                                                                      tcgAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGCGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCa           >  1:1070398/1‑140 (MQ=255)                                                                                                                       cgAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAg                   >  2:668068/1‑131 (MQ=255)                                                                                                                       cgAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAg                   <  1:668068/131‑1 (MQ=255)                                                                                                                       cgAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAg          >  1:96635/1‑140 (MQ=255)                                                                                                                        gAGGTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAgg         <  1:21953/140‑1 (MQ=255)                                                                                                                          ggTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGa                     <  2:236941/126‑1 (MQ=255)                                                                                                                          ggTATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGa                     >  1:236941/1‑126 (MQ=255)                                                                                                                            tATTTCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCt     >  1:386081/1‑140 (MQ=255)                                                                                                                               ttCGCCGCGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTc  >  2:296116/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      CGTTTAAAGTCAGTCAGAGCACGCAGCAGGCTCAGTGCTTTGGTCTTATCGATACCGATGCTGAACAGGAACAGCAGGTTATACGGACCGGTTTTCTCAACAACGATGCCATGTTCGTCGAGGTATTTCGCCACGATGCTGGCCGGAATACCAAAGTCGCTCATGGTGCCGTCTTTTTCCATCCCCGGAGTCAGCAGGGTGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTC  >  NC_000913/4356945‑4357210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑