Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,423,458	G→A	G205R (GGG→AGG)	ulaE →	L‑ribulose‑5‑phosphate 3‑epimerase UlaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,423,458	0	G	A	100.0%	312.0 / NA	87	G205R (GGG→AGG)	ulaE	L‑ribulose‑5‑phosphate 3‑epimerase UlaE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/46);  total (41/46)

ATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGCCGTACCT  >  NC_000913/4423321‑4423591                                                                                                                                          |                                                                                                                                      aTCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCagg                                                                                                                                     >  2:903634/1‑140 (MQ=255)  tCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGc                                                                                                                                    >  1:314788/1‑140 (MQ=255)  tCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGc                                                                                                                                    >  1:307077/1‑140 (MQ=255)  tCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGc                                                                                                                                    >  2:892471/1‑140 (MQ=255)  tCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTAACTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGAGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGc                                                                                                                                    >  2:746346/1‑140 (MQ=255)      ttGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCatat                                                                                                                                <  1:903634/140‑1 (MQ=255)       tgatgaGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATAtc                                                                                                                               >  1:1076707/1‑140 (MQ=255)        gatgaGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCata                                                                                                                                 >  1:595124/1‑137 (MQ=255)        gatgaGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCata                                                                                                                                 <  2:595124/137‑1 (MQ=255)           gaGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATAtcgtcg                                                                                                                           <  2:307077/140‑1 (MQ=255)           gaGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATAtcgtcg                                                                                                                           <  2:499694/140‑1 (MQ=255)             gCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTcgcg                                                                                                                         <  1:501323/140‑1 (MQ=255)             gCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTcgcg                                                                                                                         <  2:458555/140‑1 (MQ=255)              cTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCgg                                                                                                                        >  2:950054/1‑140 (MQ=255)              cTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCgg                                                                                                                        <  2:83098/140‑1 (MQ=255)              cTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCgg                                                                                                                        <  1:929452/140‑1 (MQ=255)                ccATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTa                                                                                                                      <  1:347587/140‑1 (MQ=255)                        aaGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGaa                                                                                                              >  1:57936/1‑140 (MQ=255)                         aGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGaaa                                                                                                             <  1:950054/140‑1 (MQ=255)                               tGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACAcc                                                                                                       >  2:484961/1‑140 (MQ=255)                                      cgcgCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTg                                                                                                >  2:757493/1‑140 (MQ=255)                                      cgcgCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTg                                                                                                >  2:752787/1‑140 (MQ=255)                                      cgcgCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTg                                                                                                <  1:216912/140‑1 (MQ=255)                                      cgcgCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTg                                                                                                >  2:202334/1‑140 (MQ=255)                                      cgcgCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTg                                                                                                >  2:216912/1‑140 (MQ=255)                                      cgcgCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTg                                                                                                <  2:501271/140‑1 (MQ=255)                                           aCTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTc                                                                                           <  1:202334/140‑1 (MQ=255)                                            cTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCt                                                                                          >  1:539650/1‑140 (MQ=255)                                                   aacaaTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCTGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCaaaaa                                                                                   >  1:227611/1‑140 (MQ=255)                                                     caaTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTTCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACg                                                                                 >  2:362535/1‑140 (MQ=255)                                                     caaTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACg                                                                                 >  2:741461/1‑140 (MQ=255)                                                     caaTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACg                                                                                 >  1:343605/1‑140 (MQ=255)                                                      aaTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGt                                                                                <  1:484961/140‑1 (MQ=255)                                                       aTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTg                                                                               <  1:1053681/140‑1 (MQ=255)                                                       aTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTg                                                                               >  1:143706/1‑140 (MQ=255)                                                         ccGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTg                                                                               >  1:568685/1‑138 (MQ=255)                                                         ccGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTg                                                                               <  2:568685/138‑1 (MQ=255)                                                         ccGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGcc                                                                             >  1:797636/1‑140 (MQ=255)                                                         ccGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGcc                                                                             >  2:330430/1‑140 (MQ=255)                                                         ccGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGcc                                                                             >  1:95671/1‑140 (MQ=255)                                                          cGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCg                                                                            >  1:605703/1‑140 (MQ=255)                                                               ttCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTgg                                                                       <  1:330430/140‑1 (MQ=255)                                                                  cAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGa                                                                    <  1:741461/140‑1 (MQ=255)                                                                  cAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTAGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGa                                                                    >  2:835850/1‑140 (MQ=255)                                                                    gCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAg                                                                  <  1:591728/140‑1 (MQ=255)                                                                    gCTCTACCCGAATATCGGCAACCTGTCGGCGTGGGACAACTATGTGCAGATTGATTGGCAAGCCGCAATCATTCATATCAGCGCGGTACATGTTAAAGACACCAAACCTGGCGACTTCAAAAACGTGCCGTTTGGCGAAg                                                                  <  2:409334/140‑1 (MQ=255)                                                                     ctctACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAgg                                                                 <  2:605703/140‑1 (MQ=255)                                                                        tACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGgtgt                                                              >  1:209402/1‑140 (MQ=255)                                                                         aCCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAGCGTGCCGTTTGGCGAAGGTGTa                                                             >  1:766685/1‑140 (MQ=255)                                                                         aCCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTa                                                             >  1:409334/1‑140 (MQ=255)                                                                         aCCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTa                                                             >  2:1057350/1‑140 (MQ=255)                                                                          cccGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAg                                                            >  2:32003/1‑140 (MQ=255)                                                                          cccGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAg                                                            <  2:314788/140‑1 (MQ=255)                                                                          cccGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAg                                                            >  1:458188/1‑140 (MQ=255)                                                                             ggATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTgg                                                         >  2:895731/1‑140 (MQ=255)                                                                                   cGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCg                                                   >  2:282285/1‑140 (MQ=255)                                                                                    ggCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGa                                                  >  2:791968/1‑140 (MQ=255)                                                                                         ccTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCTAACg                                               <  1:424613/138‑1 (MQ=255)                                                                                         ccTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACgtt                                             >  1:326205/1‑140 (MQ=255)                                                                                         ccTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGCGCCGTTTGGCGAAGGCGTAGTGGATTTCTAACg                                               >  2:424613/1‑138 (MQ=255)                                                                                         ccTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGCACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACgtt                                             >  1:177840/1‑140 (MQ=255)                                                                                                  gTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGaaa                                    <  1:757493/140‑1 (MQ=255)                                                                                                   tGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAAc                                   >  2:388542/1‑140 (MQ=255)                                                                                                    gggACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGTGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACg                                  <  2:1016991/140‑1 (MQ=255)                                                                                                    gggACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACg                                  <  2:766685/140‑1 (MQ=255)                                                                                                    gggACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACg                                  <  1:746346/140‑1 (MQ=255)                                                                                                    gggACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTAGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACg                                  <  1:835850/140‑1 (MQ=255)                                                                                                     ggACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGc                                 <  2:209402/140‑1 (MQ=255)                                                                                                       aCAACGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTc                               >  1:709145/1‑140 (MQ=255)                                                                                                          aCGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCaaa                            >  2:213339/1‑140 (MQ=255)                                                                                                          aCGATGTGCAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCaaa                            <  2:709145/140‑1 (MQ=255)                                                                                                                  cAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTgg                    <  2:189864/140‑1 (MQ=255)                                                                                                                  cAGATGGAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTgg                    <  2:797636/140‑1 (MQ=255)                                                                                                                        gAGTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTg              <  1:895731/140‑1 (MQ=255)                                                                                                                          gTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCg            <  2:458188/140‑1 (MQ=255)                                                                                                                          gTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCg            <  1:213339/140‑1 (MQ=255)                                                                                                                          gTTGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGACTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCg            >  2:749808/1‑140 (MQ=255)                                                                                                                            tGCAGGCCTGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCggg          <  2:227611/140‑1 (MQ=255)                                                                                                                            tGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCggg          <  2:95671/140‑1 (MQ=255)                                                                                                                            tGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCggg          <  1:605391/140‑1 (MQ=255)                                                                                                                            tGCAGGCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCggg          <  1:362535/140‑1 (MQ=255)                                                                                                                                 gCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGCCGTa     <  2:326205/140‑1 (MQ=255)                                                                                                                                 gCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGCCGTa     <  1:749808/140‑1 (MQ=255)                                                                                                                                 gCCGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGCCGTa     <  1:282285/140‑1 (MQ=255)                                                                                                                                   cGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGCCGTAcc   <  2:143706/140‑1 (MQ=255)                                                                                                                                   cGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGCCGTAcc   <  2:1076707/140‑1 (MQ=255)                                                                                                                                   cGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGCCGTAcc   <  1:227048/140‑1 (MQ=255)                                                                                                                                   cGGAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGCCGTAcc   <  1:1057350/140‑1 (MQ=255)                                                                                                                                    ggAATCAGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGCCGTACCt  <  2:177840/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      ATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGCCGTACCT  >  NC_000913/4423321‑4423591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑