Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,464,536	A→G	S58S (TCT→TCC)	treC ←	trehalose‑6‑phosphate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,464,536	0	A	G	100.0%	279.4 / NA	78	S58S (TCT→TCC)	treC	trehalose‑6‑phosphate hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/39);  total (39/39)

AAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGA  >  NC_000913/4464401‑4464672                                                                                                                                        |                                                                                                                                         aaaCACCATATCGCGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGgggaga                                                                                                                                      >  2:547930/1‑140 (MQ=255)  aaCACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGAc                                                                                                                                     >  2:957229/1‑140 (MQ=255)   acacCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGgggaggca                                                                                                                                    <  1:294453/140‑4 (MQ=255)   acacCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCa                                                                                                                                    >  1:779503/1‑140 (MQ=255)        atatCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGAc                                                                                                                                     >  1:295433/1‑134 (MQ=255)        atatCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGAc                                                                                                                                     <  2:295433/134‑1 (MQ=255)        atatCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATaaaa                                                                                                                               >  2:318695/1‑140 (MQ=255)        atatCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATaaaa                                                                                                                               >  1:811542/1‑140 (MQ=255)        atatCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATaaaa                                                                                                                               >  1:884208/1‑140 (MQ=255)         tatCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAg                                                                                                                              >  2:667909/1‑140 (MQ=255)                aaTGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTa                                                                                                                       >  1:248741/1‑140 (MQ=255)                 aTGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAg                                                                                                                      >  2:251114/1‑140 (MQ=255)                  tGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGc                                                                                                                     <  2:779503/140‑1 (MQ=255)                    aTACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTTGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATaaaa                                                                                                                               <  1:149217/128‑1 (MQ=255)                    aTACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATaaaa                                                                                                                               >  2:149217/1‑128 (MQ=255)                     tACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATaaaa                                                                                                                               <  2:476786/127‑1 (MQ=255)                     tACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATaaaa                                                                                                                               >  1:476786/1‑127 (MQ=255)                     tACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAg                                                                                                                  >  2:419697/1‑140 (MQ=255)                     tACGAATCCCGCGCGATTTTGCCTGCGTAACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAg                                                                                                                  >  2:271989/1‑140 (MQ=255)                      aCGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGa                                                                                                                 <  2:538408/140‑1 (MQ=255)                         aaTCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATaaaa                                                                                                                               <  2:469221/123‑1 (MQ=255)                         aaTCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATaaaa                                                                                                                               >  1:469221/1‑123 (MQ=255)                         aaTCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATgg                                                                                                              <  1:419697/140‑1 (MQ=255)                             ccGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATc                                                                                                          <  1:318695/140‑1 (MQ=255)                              cgcgcgATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCa                                                                                                         >  2:679001/1‑140 (MQ=255)                              cgcgcgATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCa                                                                                                         >  2:365184/1‑140 (MQ=255)                              cgcgcgATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCa                                                                                                         >  1:257513/1‑140 (MQ=255)                              cgcgcgATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCa                                                                                                         >  2:981564/1‑140 (MQ=255)                               gcgcgATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCa                                                                                                         <  1:1086636/139‑1 (MQ=255)                               gcgcgATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCa                                                                                                         >  2:1086636/1‑139 (MQ=255)                                cgcgATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAAc                                                                                                       >  1:486088/1‑140 (MQ=255)                                cgcgATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAAc                                                                                                       >  2:1044159/1‑140 (MQ=255)                                cgcgATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAAc                                                                                                       >  1:462661/1‑140 (MQ=255)                                  cgATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGc                                                                                                     <  2:811542/140‑1 (MQ=255)                                      tttGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAg                                                                                                 <  1:839437/140‑1 (MQ=255)                                      tttGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAg                                                                                                 <  1:540947/140‑1 (MQ=255)                                      tttGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAg                                                                                                 <  1:957229/140‑1 (MQ=255)                                        tGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGtt                                                                                               <  2:921511/140‑1 (MQ=255)                                           cTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTAt                                                                                            <  2:248741/140‑1 (MQ=255)                                                taaCCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGa                                                                                       <  1:271989/138‑1 (MQ=255)                                                tCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGa                                                                                       <  2:1071829/140‑1 (MQ=255)                                                tCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGa                                                                                       <  2:884208/140‑1 (MQ=255)                                                 cACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGAt                                                                                      <  1:365184/140‑1 (MQ=255)                                                     aGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTc                                                                                  <  1:981564/140‑1 (MQ=255)                                                     aGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTc                                                                                  <  2:257513/140‑1 (MQ=255)                                                      gTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTcc                                                                                 <  1:679001/140‑1 (MQ=255)                                                      gTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTcc                                                                                 <  1:251114/140‑1 (MQ=255)                                                         cGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAgg                                                                              >  1:22905/1‑140 (MQ=255)                                                         cGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAgg                                                                              >  1:175082/1‑140 (MQ=255)                                                         cGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAgg                                                                              >  2:733584/1‑140 (MQ=255)                                                         cGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAgg                                                                              <  1:25782/140‑1 (MQ=255)                                                         cGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAgg                                                                              >  2:25782/1‑140 (MQ=255)                                                                   gTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATaa                                                                    <  2:175082/140‑1 (MQ=255)                                                                    tCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAAc                                                                   >  2:87991/1‑140 (MQ=255)                                                                    tCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAAc                                                                   <  1:87991/140‑1 (MQ=255)                                                                                   gTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAAt                                                        <  2:733981/136‑1 (MQ=255)                                                                                   gTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAAt                                                        >  1:733981/1‑136 (MQ=255)                                                                                    tGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCg                                                   <  2:22905/140‑1 (MQ=255)                                                                                        aTCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTAc                                               >  2:693143/1‑140 (MQ=255)                                                                                         tCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTAcc                                              <  1:733584/140‑1 (MQ=255)                                                                                             tCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCg                                                      <  2:897595/128‑1 (MQ=255)                                                                                             tCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCg                                                      >  1:897595/1‑128 (MQ=255)                                                                                              cGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTAc                                         <  1:1044159/140‑1 (MQ=255)                                                                                                   tataGTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCgtg                                    >  2:826084/1‑140 (MQ=255)                                                                                                       gTTCGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGgtgt                                <  1:839723/140‑1 (MQ=255)                                                                                                        ttCGCTACGGCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTc                               >  2:404405/1‑140 (MQ=255)                                                                                                          cGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTcc                              >  1:449053/1‑139 (MQ=255)                                                                                                          cGCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTcc                              <  2:449053/139‑1 (MQ=255)                                                                                                           gCTACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTg                            >  2:491206/1‑140 (MQ=255)                                                                                                             tACGTCGTAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGaa                          >  1:1018096/1‑140 (MQ=255)                                                                                                                  cgtAACCGTTATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAActc                     >  2:215297/1‑140 (MQ=255)                                                                                                                   gtAACCGTTATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAActct                    <  1:547930/140‑1 (MQ=255)                                                                                                                           tATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATaa            >  1:295276/1‑140 (MQ=255)                                                                                                                           tATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATaa            <  1:215297/140‑1 (MQ=255)                                                                                                                           tATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATaa            <  2:314231/140‑1 (MQ=255)                                                                                                                           tATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATaa            >  1:1048837/1‑140 (MQ=255)                                                                                                                           tATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATaa            <  2:1018096/140‑1 (MQ=255)                                                                                                                           tATCGACCTGGGGGGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGGGTCCTGAAAACTCTTTGGATaa            >  1:13123/1‑140 (MQ=255)                                                                                                                           tATCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATaa            <  2:462661/140‑1 (MQ=255)                                                                                                                            aTCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCtt                   >  1:531786/1‑132 (MQ=255)                                                                                                                            aTCGACCTGGGGGGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCtt                   <  2:531786/132‑1 (MQ=255)                                                                                                                                     ggggGAGGCATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGa  <  2:1048837/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         AAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGA  >  NC_000913/4464401‑4464672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑