Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,482,676	C→T	V388I (GTC→ATC)	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,482,676	0	C	T	98.4%	218.1 / ‑4.4	63	V388I (GTC→ATC)	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (29/33);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

ACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACT  >  NC_000913/4482542‑4482809                                                                                                                                       |                                                                                                                                      cccccccCACAACTGACGAGAGATACACCAGTCCTGAATACCGCGCATCCAGGAGAAGTACATTTTTTCGTACTGCTTCGGTACGAACGGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCgg                                                                                                                                  <  2:744887/139‑1 (MQ=255) aCCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCgg                                                                                                                                  >  1:1021196/1‑140 (MQ=255) aCCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCgg                                                                                                                                  <  1:314709/140‑1 (MQ=255)   cccACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGgca                                                                                                                                <  1:671338/140‑1 (MQ=255)    ccaccaCAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGgcac                                                                                                                               <  1:611860/140‑1 (MQ=255)     caccacAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGTCGGTTTCGCCAGGATATCGgcacg                                                                                                                              <  2:94592/140‑1 (MQ=255)      accacAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGgcacgc                                                                                                                             >  2:884504/1‑140 (MQ=255)       ccaaAACTGAAGGGAGATACCCCAGTCCTGAATATCGCCCACCAGGGAGAAGTACATTTTTCCTTACTGTTGCGGTACGAACTGAATTTCGCCGTTCCCAACCGCTTAACCCGCCGGTTTCGCCAGGATATCGgcacgca                                                                                                                            <  2:127665/140‑1 (MQ=255)             cTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTAcc                                                                                                                      >  1:127665/1‑140 (MQ=255)                  aagagaTACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACTTGTTTTCGTCCTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCTGTTTCGCCAGGATATCGGCACGCACGTACCACTgg                                                                                                                 <  1:414991/139‑1 (MQ=255)                       aTACCCCAGTCCTGAATATCGCCCACCCAGGAGAAGTACATGTTTTCGTACTGCTTGGGTCCGAACTGAATGTCGCCGTTCTCAGCCGCTTCATCCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTggtcggt                                                                                                            <  2:395268/140‑1 (MQ=255)                       aTACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCACCCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTggtcggt                                                                                                            <  1:465084/140‑1 (MQ=255)                       aTACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTggtcggt                                                                                                            <  1:874816/140‑1 (MQ=255)                       aTACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTggtcggt                                                                                                            <  2:362202/140‑1 (MQ=255)                            ccAGCCCTGAATATCGCGCATCCAGGAGAAGTACATTTTTTCGTACTGCTTCGGTACGAACTAAATGCCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCa                                                                                                       <  2:548592/140‑1 (MQ=255)                               gTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTg                                                                                                    >  1:887544/1‑140 (MQ=255)                               gTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTg                                                                                                    >  1:998961/1‑140 (MQ=255)                                tCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCAtt                                                                                                     <  2:747527/138‑1 (MQ=255)                                tCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCAtt                                                                                                     >  1:747527/1‑138 (MQ=255)                                tCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTgg                                                                                                   >  1:598116/1‑140 (MQ=255)                                tCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTgg                                                                                                   >  2:401675/1‑140 (MQ=255)                                 ccTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGt                                                                                                  >  2:1050009/1‑140 (MQ=255)                                 ccTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGt                                                                                                  >  1:710967/1‑140 (MQ=255)                                 ccTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGt                                                                                                  >  2:721095/1‑140 (MQ=255)                                  ttGAATACCGCGCAGCCAGGAGAATTACTTGTTTACGTCCTGCTTCGGTACGACCTAAATGTCGCCGTTCCCAACCTCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGtt                                                                                                 <  2:160497/139‑1 (MQ=255)                                        atCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATaa                                                                                           <  2:710967/140‑1 (MQ=255)                                          cgcgCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACt                                                                                         >  1:1024608/1‑140 (MQ=255)                                          cgcgCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACt                                                                                         >  1:895905/1‑140 (MQ=255)                                          cgcgCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACt                                                                                         >  1:762556/1‑140 (MQ=255)                                          cgcgCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACt                                                                                         >  1:160497/1‑140 (MQ=255)                                           gcgcATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTa                                                                                        <  2:598116/140‑1 (MQ=255)                                            cgcATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTAc                                                                                       >  1:763004/1‑140 (MQ=255)                                            cgcATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTAc                                                                                       >  2:254555/1‑140 (MQ=255)                                            cgcATCCAGGAGAAGTACATGTTTTCGTACTGCTACGGTACGAACTGAATGTCGCCGTTCTCAACCACTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTAc                                                                                       >  2:963289/1‑140 (MQ=255)                                                      aaaaGTACTTTTTTTCTTACTGTTTCGTTACAAACTAAATGTCCCCGTTCCCACCCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACgg                                                                             <  2:271576/138‑1 (MQ=255)                                                       gaAGTACATTTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCTTTCCCACCCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGt                                                                            <  2:762556/140‑1 (MQ=255)                                                       gaAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGt                                                                            <  2:73805/140‑1 (MQ=255)                                                           tACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGcc                                                                        >  1:905829/1‑140 (MQ=255)                                                           tACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGcc                                                                        >  2:416056/1‑140 (MQ=255)                                                           tACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGcc                                                                        >  1:161819/1‑140 (MQ=255)                                                                 ttttCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAgg                                                                  <  2:887544/140‑1 (MQ=255)                                                                 ttttCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAgg                                                                  <  2:161819/140‑1 (MQ=255)                                                                 ttttCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAgg                                                                  <  1:254555/140‑1 (MQ=255)                                                                 ttttCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCACTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAgg                                                                  <  1:963289/140‑1 (MQ=255)                                                                  tttCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGa                                                                 <  2:895905/140‑1 (MQ=255)                                                                   ttCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGaa                                                                <  2:905829/140‑1 (MQ=255)                                                                             ttCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTc                                                      <  1:401675/140‑1 (MQ=255)                                                                                      aaCTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGttt                                             >  1:922740/1‑140 (MQ=255)                                                                                        cTGAATGTCGCCTTTCTCACCCGTTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTaa                                           <  2:1024608/140‑1 (MQ=255)                                                                                        cTGAATGCCGCCGTCCTAACCCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTaa                                           <  2:469564/140‑1 (MQ=255)                                                                                           aaTGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAAttt                                        >  2:200565/1‑140 (MQ=255)                                                                                           aaTGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGCTTAAttt                                        >  1:598832/1‑140 (MQ=255)                                                                                            aTGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATttc                                       >  2:569369/1‑140 (MQ=255)                                                                                                 gCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCca                                  >  2:1034391/1‑140 (MQ=255)                                                                                                  ccGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCcag                                 <  2:922740/140‑1 (MQ=255)                                                                                                   cGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCcagc                                >  1:813607/1‑140 (MQ=255)                                                                                                         cAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCa                          >  2:1041581/1‑140 (MQ=255)                                                                                                         cAACCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGGCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCa                          <  1:721095/140‑1 (MQ=255)                                                                                                           aCCGCTTCAACCGCCGGTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAg                        >  1:214035/1‑140 (MQ=255)                                                                                                                          ggTTTCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGc         <  1:1074155/140‑1 (MQ=255)                                                                                                                              tCGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACg     <  2:998961/140‑1 (MQ=255)                                                                                                                               cgacAGGATATCGGCACGCACGTACCGCTGGTCGGTCAGCATTGGTGCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGa    <  2:921458/137‑1 (MQ=255)                                                                                                                               cGCCAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGa    <  1:884504/140‑1 (MQ=255)                                                                                                                                 ccAGGATATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACt  <  2:598832/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      ACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACT  >  NC_000913/4482542‑4482809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑