Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	407,368	C→T	intergenic (+198/‑60)	yaiA → / → aroM	protein YaiA/protein AroM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	407,368	0	C	T	100.0%	285.9 / NA	80	intergenic (+198/‑60)	yaiA/aroM	protein YaiA/protein AroM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/41);  total (39/41)

CATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAA  >  NC_000913/407233‑407499                                                                                                                                        |                                                                                                                                    cATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAgtg                                                                                                                                 <  1:372035/140‑1 (MQ=255)  aTAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAgtgg                                                                                                                                <  1:255282/140‑1 (MQ=255)  aTAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAgtgg                                                                                                                                <  1:22573/140‑1 (MQ=255)                 cTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGcc                                                                                                                 <  1:897986/140‑1 (MQ=255)                 cTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGcc                                                                                                                 <  1:977082/140‑1 (MQ=255)                            aaTATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAac                                                                                                      >  1:170201/1‑140 (MQ=255)                                 tAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAAcgacga                                                                                                 >  1:537338/1‑140 (MQ=255)                                 tAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAAcgacga                                                                                                 >  1:403340/1‑140 (MQ=255)                                   aacaataacaatCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGcc                                                                                                                 <  2:1067661/122‑1 (MQ=255)                                   aacaataacaatCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGcc                                                                                                                 >  1:1067661/1‑122 (MQ=255)                                   aacaataacaatCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACgaaa                                                                                               >  1:776604/1‑140 (MQ=255)                                              tCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAgg                                                                                    >  2:336753/1‑140 (MQ=255)                                              tCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAgg                                                                                    <  1:919565/140‑1 (MQ=255)                                              tCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAgg                                                                                    <  2:247665/140‑1 (MQ=255)                                                cATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTg                                                                                  >  1:576543/1‑140 (MQ=255)                                                  ttACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGa                                                                                <  2:776604/140‑1 (MQ=255)                                                      tGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGaa                                                                            >  2:542930/1‑140 (MQ=255)                                                       ggAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAAc                                                                           <  2:50100/140‑1 (MQ=255)                                                          aTCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAAt                                                                        <  2:576543/140‑1 (MQ=255)                                                             aTTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGa                                                                                <  2:317186/129‑1 (MQ=255)                                                             aTTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGa                                                                                >  1:317186/1‑129 (MQ=255)                                                             aTTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAg                                                                     >  2:297304/1‑140 (MQ=255)                                                             aTTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAg                                                                     >  1:1044946/1‑140 (MQ=255)                                                               ttGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTg                                                                   >  1:771043/1‑140 (MQ=255)                                                                   aaTCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGcgtc                                                               <  2:1044946/140‑1 (MQ=255)                                                                       tttACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTg                                                           >  2:664037/1‑140 (MQ=255)                                                                        ttACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTgg                                                          <  1:336753/140‑1 (MQ=255)                                                                         tACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGc                                                         >  2:81829/1‑140 (MQ=255)                                                                          aCATTATGCCGTGGACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCg                                                        <  1:246984/140‑1 (MQ=255)                                                                                tGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTg                                                  >  1:515161/1‑140 (MQ=255)                                                                                tGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTg                                                  <  1:1023235/140‑1 (MQ=255)                                                                                tGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTg                                                  >  2:377408/1‑140 (MQ=255)                                                                                 gCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTgg                                                          >  2:158477/1‑131 (MQ=255)                                                                                 gCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTgg                                                          <  1:158477/131‑1 (MQ=255)                                                                                 gCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGa                                                 >  2:62347/1‑140 (MQ=255)                                                                                 gCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGa                                                 >  2:423502/1‑140 (MQ=255)                                                                                 gCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGa                                                 >  1:670479/1‑140 (MQ=255)                                                                                     tGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTg                                                  <  1:932576/135‑1 (MQ=255)                                                                                     tGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTg                                                  >  2:932576/1‑135 (MQ=255)                                                                                     tGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCAt                                             <  2:403340/140‑1 (MQ=255)                                                                                     tGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCAt                                             >  2:927077/1‑140 (MQ=255)                                                                                     tGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCAt                                             >  2:961763/1‑140 (MQ=255)                                                                                     tGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCAt                                             >  2:709742/1‑140 (MQ=255)                                                                                     tGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCAt                                             >  2:62663/1‑140 (MQ=255)                                                                                     tGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCAt                                             >  2:976941/1‑140 (MQ=255)                                                                                     tGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCAt                                             <  1:664037/140‑1 (MQ=255)                                                                                     tGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCAt                                             >  1:79196/1‑140 (MQ=255)                                                                                     tGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCAt                                             <  1:81829/140‑1 (MQ=255)                                                                                     tGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCAt                                             >  1:363480/1‑140 (MQ=255)                                                                                     tGCACGTCTGCTGCTACGC‑TTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATc                                            <  1:155466/140‑1 (MQ=255)                                                                                      gCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAATGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATc                                            >  2:951901/1‑140 (MQ=255)                                                                                      gCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATc                                            >  2:127411/1‑140 (MQ=255)                                                                                      gCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATc                                            <  1:62347/140‑1 (MQ=255)                                                                                      gCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATc                                            <  2:936281/140‑1 (MQ=255)                                                                                       cACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCg                                           >  2:1015589/1‑140 (MQ=255)                                                                                       cACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCg                                           <  2:670479/140‑1 (MQ=255)                                                                                        aCGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCgg                                          <  1:709742/140‑1 (MQ=255)                                                                                          gTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCa                                        <  1:961763/140‑1 (MQ=255)                                                                                          gTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCa                                        >  2:1048238/1‑140 (MQ=255)                                                                                          gTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCa                                        <  2:696193/140‑1 (MQ=255)                                                                                                  ctACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCt                                >  1:367634/1‑140 (MQ=255)                                                                                                       cTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCa                           <  2:367634/140‑1 (MQ=255)                                                                                                                  ttGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGc                >  1:991093/1‑140 (MQ=255)                                                                                                                  ttGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGc                <  2:1007841/140‑1 (MQ=255)                                                                                                                   tGTAGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGcc               <  2:771043/140‑1 (MQ=255)                                                                                                                      aGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCt            <  1:542930/140‑1 (MQ=255)                                                                                                                      aGCACAAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCt            <  2:363480/140‑1 (MQ=255)                                                                                                                        cacaAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTcc          >  2:118613/1‑140 (MQ=255)                                                                                                                         acaAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCt         <  1:118613/140‑1 (MQ=255)                                                                                                                          caAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTg        <  2:537338/140‑1 (MQ=255)                                                                                                                          caAGTAAGTGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTg        <  1:992675/140‑1 (MQ=255)                                                                                                                           aagtaagtGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGa       <  2:343027/140‑1 (MQ=255)                                                                                                                             gtaagtGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTcc          >  1:4611/1‑135 (MQ=255)                                                                                                                             gtaagtGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTcc          <  2:4611/135‑1 (MQ=255)                                                                                                                             gtaagtGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACg     >  2:337354/1‑140 (MQ=255)                                                                                                                                agtGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGaa  <  2:515161/140‑1 (MQ=255)                                                                                                                                agtGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGaa  >  1:157480/1‑140 (MQ=255)                                                                                                                                agtGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGaa  <  1:1048238/140‑1 (MQ=255)                                                                                                                                agtGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGa   <  1:40049/139‑1 (MQ=255)                                                                                                                                agtGTCAGTAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGa   >  2:40049/1‑139 (MQ=255)                                                                                                                                     cagtagTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTg        <  1:853699/129‑1 (MQ=255)                                                                                                                                     cagtagTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTg        >  2:853699/1‑129 (MQ=255)                                                                                                                                        |                                                                                                                                    CATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAA  >  NC_000913/407233‑407499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑