Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	517,217	T→C	V212A (GTG→GCG)	fetB →	putative iron ABC exporter membrane subunit FetB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	517,217	0	T	C	100.0%	180.7 / NA	51	V212A (GTG→GCG)	fetB	putative iron ABC exporter membrane subunit FetB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/28);  total (23/28)

GGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTG  >  NC_000913/517086‑517350                                                                                                                                    |                                                                                                                                      ggCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGa                                                                                                                               <  1:965447/140‑1 (MQ=255)    ttCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTa                                                                                                                            <  1:1007619/140‑1 (MQ=255)    ttCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTa                                                                                                                            >  2:507907/1‑140 (MQ=255)    ttCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTa                                                                                                                            >  1:219680/1‑140 (MQ=255)    ttCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTa                                                                                                                            <  1:736157/140‑1 (MQ=255)      cAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTaaa                                                                                                                          >  2:956488/1‑140 (MQ=255)        gCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAata                                                                                                                        <  2:463380/140‑1 (MQ=255)        gCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAata                                                                                                                        <  1:956488/140‑1 (MQ=255)         cGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAatat                                                                                                                       >  2:1819/1‑140 (MQ=255)             atTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGa                                                                                                                   <  1:689974/140‑1 (MQ=255)                gATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTa                                                                                                                <  1:958724/140‑1 (MQ=255)                    cgcgACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGt                                                                                                            <  2:1059866/140‑1 (MQ=255)                    cgcgACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGt                                                                                                            <  2:1051783/140‑1 (MQ=255)                    cgcgACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGt                                                                                                            <  2:1051782/140‑1 (MQ=255)                      cgACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGa                                                                                                          <  1:832978/140‑1 (MQ=255)                      cgACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGa                                                                                                          >  2:302208/1‑140 (MQ=255)                      cgACAGTATTCGCGCGACTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGa                                                                                                          >  2:695044/1‑140 (MQ=255)                              ttCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTAtg                                                                                                  >  1:970386/1‑140 (MQ=255)                                 gcgcgGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTAtgctg                                                                                               <  1:302208/140‑1 (MQ=255)                                    cgGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCt                                                                                                       <  2:206796/129‑1 (MQ=255)                                    cgGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCt                                                                                                       >  1:206796/1‑129 (MQ=255)                                    cgGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGctc                                                                                            >  1:799208/1‑140 (MQ=255)                                    cgGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGctc                                                                                            >  1:927114/1‑140 (MQ=255)                                      gCTTTACTTCCGACGGACGATTCAGCAAATCCGGTTGCCTTAGTGAGCTTACCAGGAATGATGTCCGGGCTCCTATTTGCCGGGATCGATCCGCTCAGGGCGCTTAAATATCAGCTTATGGTGCCCTTTATGCTGCtctc                                                                                          >  2:119793/1‑140 (MQ=255)                                      gCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCtctc                                                                                          >  2:931738/1‑140 (MQ=255)                                                 gACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCt                                                                               >  1:966741/1‑140 (MQ=255)                                                       cGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGa                                                                         <  2:1038811/140‑1 (MQ=255)                                                       cGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGa                                                                         <  1:780343/140‑1 (MQ=255)                                                                  aaaCGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTTATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGcc                                                              <  2:173864/140‑1 (MQ=255)                                                                   aaCGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCt                                                             <  1:695044/140‑1 (MQ=255)                                                                    aCGGTTGGATTAGTGAGTTTACTAGGAATGATTCCGGGGTTGATTTTTGCCGGGGTTGATCCGGCGAGGGTGATTAATTATGAGATTGTGGTGACTTTTATGCTGCTTTCAACCGCCAGCTTGTCGACCATTATTGCCTg                                                            <  2:532435/140‑1 (MQ=255)                                                                        ttGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTAt                                                        <  1:126033/140‑1 (MQ=255)                                                                         tGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTAtt                                                       >  2:471382/1‑140 (MQ=255)                                                                           gCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTa                                                     >  1:1082551/1‑140 (MQ=255)                                                                             ttAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAAc                                                   <  2:1082551/140‑1 (MQ=255)                                                                                    gTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGt                                            <  2:927114/140‑1 (MQ=255)                                                                                     tttACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTa                                           >  1:523375/1‑140 (MQ=255)                                                                                        aCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGt                                        >  1:933734/1‑140 (MQ=255)                                                                                               atgatgTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATaa                                 <  1:1819/140‑1 (MQ=255)                                                                                                  atgTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTc                              >  1:19345/1‑140 (MQ=255)                                                                                                   tgTCCGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTcg                             <  2:19345/140‑1 (MQ=255)                                                                                                       cGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGcc                         >  2:1038095/1‑140 (MQ=255)                                                                                                       cGGGCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGcc                         >  1:320017/1‑140 (MQ=255)                                                                                                         ggCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGccac                       <  2:933734/140‑1 (MQ=255)                                                                                                         ggCTGATATTTGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGccac                       <  1:931738/140‑1 (MQ=255)                                                                                                                 tttGCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTtggt               <  1:507907/140‑1 (MQ=255)                                                                                                                    gCCGGGATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTggtggt            >  1:389565/1‑140 (MQ=255)                                                                                                                       gggATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTACGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGAc         <  1:1038095/140‑1 (MQ=255)                                                                                                                         gATTGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGc       >  1:357670/1‑140 (MQ=255)                                                                                                                           ttGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTTTCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCaa     <  2:966741/140‑1 (MQ=255)                                                                                                                            tGATCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAAt    <  2:357670/140‑1 (MQ=255)                                                                                                                              aTCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGTTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTg  >  2:85916/1‑140 (MQ=255)                                                                                                                              aTCCGGCGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTg  >  2:891476/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                      GGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTG  >  NC_000913/517086‑517350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑