Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,734	C→T	P310S (CCA→TCA)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,734	0	C	T	100.0%	194.8 / NA	55	P310S (CCA→TCA)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/27);  total (28/27)

TGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGG  >  NC_000913/46601‑46861                                                                                                                                      |                                                                                                                                tGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCAttgt                                                                                                                           >  1:990444/1‑140 (MQ=255)   gCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCAttgttg                                                                                                                         >  1:218822/1‑140 (MQ=255)        cAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTgg                                                                                                                    >  1:406112/1‑140 (MQ=255)        cAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTgg                                                                                                                    <  1:862803/140‑1 (MQ=255)                             ttGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGAcgct                                                                                               >  2:289564/1‑140 (MQ=255)                                 gggTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGAcgctggcg                                                                                           >  2:944588/1‑140 (MQ=255)                                 gggTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGAcgctggcg                                                                                           >  2:1018565/1‑140 (MQ=255)                                  ggTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGAcgctggcgc                                                                                          <  2:406112/140‑1 (MQ=255)                                    ttGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGAcgctggcgctg                                                                                        >  1:6338/1‑140 (MQ=255)                                     tGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGAcgctggcgctgg                                                                                       <  1:944588/140‑1 (MQ=255)                                     tGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGAcgctggcgctgg                                                                                       <  2:503107/140‑1 (MQ=255)                                     tGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGAcgctggcgctgg                                                                                       >  2:396673/1‑140 (MQ=255)                                      ggCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGTCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACgctggcgctggc                                                                                      >  2:355391/1‑140 (MQ=255)                                      ggCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACgctggcgctggc                                                                                      >  2:227087/1‑140 (MQ=255)                                      ggCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACgctggcgctggc                                                                                      >  1:765346/1‑140 (MQ=255)                                      ggCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTCCCATGATGACgctggcgctggc                                                                                      >  2:240283/1‑140 (MQ=255)                                                cgGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTgggg                                                                            <  2:934695/140‑1 (MQ=255)                                                cgGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTgggg                                                                            <  1:779920/140‑1 (MQ=255)                                                 ggCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGc                                                                           <  2:547428/140‑1 (MQ=255)                                                      tAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGAGGTTTTGGGGCTAATc                                                                      >  2:973036/1‑140 (MQ=255)                                                        gggAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGTCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATccc                                                                    <  1:355391/140‑1 (MQ=255)                                                         ggAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCg                                                                   <  2:461264/140‑1 (MQ=255)                                                          gAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCgg                                                                  <  2:6338/140‑1 (MQ=255)                                                          gAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGAACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCgg                                                                  <  2:754603/140‑1 (MQ=255)                                                               tggtgATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATg                                                             >  1:607763/1‑140 (MQ=255)                                                                ggtgATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGc                                                                           >  1:934723/1‑125 (MQ=255)                                                                ggtgATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGc                                                                           <  2:934723/125‑1 (MQ=255)                                                                ggtgATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATgg                                                            <  1:973036/140‑1 (MQ=255)                                                                  tgATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAAt                                                                       >  1:347002/1‑127 (MQ=255)                                                                  tgATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAAt                                                                       <  2:347002/127‑1 (MQ=255)                                                                   gATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCt                                                                          >  1:33865/1‑123 (MQ=255)                                                                   gATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCt                                                                          <  2:33865/123‑1 (MQ=255)                                                                         ccTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATctggc                                                   <  1:861923/140‑1 (MQ=255)                                                                           tGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATctggctg                                                 >  1:606448/1‑140 (MQ=255)                                                                            gTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGAt                                                        >  1:695706/1‑132 (MQ=255)                                                                            gTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGAt                                                        <  2:695706/132‑1 (MQ=255)                                                                            gTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATctggctgg                                                <  1:1018565/140‑1 (MQ=255)                                                                                   aTGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGAt                                                        <  1:69701/125‑1 (MQ=255)                                                                                   aTGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGAt                                                        >  2:69701/1‑125 (MQ=255)                                                                                      cTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGc                                      <  2:606448/140‑1 (MQ=255)                                                                                       tCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGGTATGGGGATCTGGGTGGGGGTGATGGcc                                     >  1:513829/1‑140 (MQ=255)                                                                                         ggCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATctggct                                                  >  2:714162/1‑125 (MQ=255)                                                                                         ggCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATctggct                                                  <  1:714162/125‑1 (MQ=255)                                                                                          gCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCttt                                  <  2:13181/140‑1 (MQ=255)                                                                                           cTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCTGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTg                                 <  2:990444/140‑1 (MQ=255)                                                                                             gTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCg                               <  1:227087/140‑1 (MQ=255)                                                                                             gTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTCCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCg                               <  1:240283/140‑1 (MQ=255)                                                                                              tATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTg                                 >  2:675156/1‑137 (MQ=255)                                                                                              tATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTg                                 <  1:675156/137‑1 (MQ=255)                                                                                                     ccgATGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGGTCTGGCTGGGAGTGATGGCCTTTGCGGTGGATGc                       >  2:774375/1‑140 (MQ=255)                                                                                                        aTGCTGTGGTTAAACACTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGGTATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCtt                    >  2:56670/1‑140 (MQ=255)                                                                                                                     acacTGCCGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTTGGGCTAATCCCGGGTATGGGGGTCTGGCTGGTAGTGATGGCCTTTGCGGGGTATGCCTTTTTTTCTCGCggg       >  2:811515/1‑140 (MQ=255)                                                                                                                          gCCGGACGGCGTTCATTGTAGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGGTCTGGCTGGGAATGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCggg  >  2:1007079/1‑140 (MQ=255)                                                                                                                           ccGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTTTCTGGCggg       >  2:60410/1‑134 (MQ=255)                                                                                                                           ccGGACGGCGTTCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCggg       <  1:60410/134‑1 (MQ=255)                                                                                                                                      |                                                                                                                                TGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGG  >  NC_000913/46601‑46861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑