Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	713,628	C→T	A24V (GCG→GTG)	pgm →	phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	713,628	0	C	T	100.0%	166.2 / NA	48	A24V (GCG→GTG)	pgm	phosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/20);  total (28/20)

TCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAG  >  NC_000913/713492‑713755                                                                                                                                         |                                                                                                                                tCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCa                                                                                                                              <  1:814769/140‑1 (MQ=255) tCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCa                                                                                                                              <  2:698228/140‑1 (MQ=255)    cggcggAATTGATTGAGAAGGTTTGCGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAata                                                                                                                           <  1:707157/140‑1 (MQ=255)      gcggAATTGATTGAGAAGGTTTGCGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAAtatt                                                                                                                         <  1:1064407/140‑1 (MQ=255)                             cGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAggg                                                                                                  >  1:986878/1‑140 (MQ=255)                             cGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAggg                                                                                                  >  2:449495/1‑140 (MQ=255)                             cGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAggg                                                                                                  <  2:875000/140‑1 (MQ=255)                                 aCTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATg                                                                                              <  2:871107/140‑1 (MQ=255)                                              ttGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGt                                                                                 >  2:380430/1‑140 (MQ=255)                                                      aaaggacaaagCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCg                                                                         >  1:30566/1‑140 (MQ=255)                                                         ggacaaagCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTa                                                                      <  2:586684/140‑1 (MQ=255)                                                              aagCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACt                                                                    >  2:1024047/1‑137 (MQ=255)                                                              aagCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACt                                                                    <  1:1024047/137‑1 (MQ=255)                                                               agCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCg                                                                >  2:429568/1‑140 (MQ=255)                                                               agCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCg                                                                <  1:380430/140‑1 (MQ=255)                                                                 cAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGt                                                              <  2:30566/140‑1 (MQ=255)                                                                    tGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCAc                                                           >  2:482468/1‑140 (MQ=255)                                                                    tGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCAc                                                           >  2:53259/1‑140 (MQ=255)                                                                    tGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCAc                                                           <  1:630004/140‑1 (MQ=255)                                                                       cAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGt                                                        >  1:1076473/1‑140 (MQ=255)                                                                         aTCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCgg                                                                        <  1:270754/122‑1 (MQ=255)                                                                         aTCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCgg                                                                        >  2:270754/1‑122 (MQ=255)                                                                         aTCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTgg                                                      >  1:126665/1‑140 (MQ=255)                                                                                    tGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAGATTCGGTACTTCCGGTCACCGTGGCAGTGCAgcgc                                           >  2:747980/1‑140 (MQ=255)                                                                                    tGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAgcgc                                           >  2:497474/1‑140 (MQ=255)                                                                                    tGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAgcgc                                           >  1:1014600/1‑140 (MQ=255)                                                                                        ggCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCca                                       <  1:429568/140‑1 (MQ=255)                                                                                         gCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCcac                                      >  2:728446/1‑140 (MQ=255)                                                                                           cAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAg                                    >  2:772962/1‑140 (MQ=255)                                                                                                       caGAGTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGcc                        >  2:1004108/1‑140 (MQ=255)                                                                                                        agagTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCg                       >  2:157321/1‑140 (MQ=255)                                                                                                         gagTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGc                      <  1:1004108/140‑1 (MQ=255)                                                                                                          agTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGca                     >  2:66474/1‑140 (MQ=255)                                                                                                          agTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGca                     >  2:937853/1‑140 (MQ=255)                                                                                                          agTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGca                     >  1:232920/1‑140 (MQ=255)                                                                                                           gTGATTTGATTAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGcgc                    <  1:449495/140‑3 (MQ=255)                                                                                                            tgatttgattAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGcaca                   >  2:1039156/1‑140 (MQ=255)                                                                                                                 tgattAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTg              >  2:90906/1‑140 (MQ=255)                                                                                                                   attAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGc            >  1:169685/1‑140 (MQ=255)                                                                                                                    ttAACGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCg           <  2:460452/140‑1 (MQ=255)                                                                                                                      aaCGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGAt         >  2:474966/1‑140 (MQ=255)                                                                                                                        cGTCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCg       >  2:412253/1‑140 (MQ=255)                                                                                                                          tCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCt     <  2:232920/140‑1 (MQ=255)                                                                                                                          tCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCt     <  2:126665/140‑1 (MQ=255)                                                                                                                          tCGCCCAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCt     <  1:482468/140‑1 (MQ=255)                                                                                                                             cccAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGc      >  2:322967/1‑136 (MQ=255)                                                                                                                             cccAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGc      <  1:322967/136‑1 (MQ=255)                                                                                                                             cccAACTGACGGTGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAg  >  2:963108/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                TCCCGGCGGAATTGATTGAGAAGGTTTGCGGAACTATCTAAAACGTTGCAGACAAAGGACAAAGCAATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCCCAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAG  >  NC_000913/713492‑713755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑