Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,100,871	A→G	S272P (TCG→CCG)	csgG ←	curli secretion channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,100,871	0	A	G	100.0%	195.6 / NA	55	S272P (TCG→CCG)	csgG	curli secretion channel
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/26);  total (29/26)

CGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTAATCAGGAAAATGACCCCTGTTTCGATAGCCGACATCAGGCACAGCATA  >  NC_000913/1100735‑1101004                                                                                                                                         |                                                                                                                                      cgcgTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCat                                                                                                                                    >  2:5738/1‑140 (MQ=255) cgcgTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCat                                                                                                                                    >  2:507068/1‑140 (MQ=255)     tAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATgg                                                                                                                                <  2:565121/140‑1 (MQ=255)      aGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGc                                                                                                                               <  2:523321/140‑1 (MQ=255)             aGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTAttt                                                                                                                        <  2:188030/140‑1 (MQ=255)             aGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTAttt                                                                                                                        >  1:896802/1‑140 (MQ=255)              gACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTc                                                                                                                       >  1:446462/1‑140 (MQ=255)                  gcgcACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTa                                                                                                                           <  2:988379/132‑1 (MQ=255)                  gcgcACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTa                                                                                                                           >  1:988379/1‑132 (MQ=255)                  gcgcACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCa                                                                                                                   <  2:173487/140‑1 (MQ=255)                  gcgcACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCa                                                                                                                   >  2:351966/1‑140 (MQ=255)                  gcgcACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCa                                                                                                                   <  1:605225/140‑1 (MQ=255)                  gcgcACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCa                                                                                                                   <  1:552949/140‑1 (MQ=255)                  gcgcACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCa                                                                                                                   >  1:224214/1‑140 (MQ=255)                          tGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCGCCAGAATGTCa                                                                                                           <  2:533024/140‑1 (MQ=255)                            aGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGCCAtt                                                                                                         >  2:267534/1‑140 (MQ=255)                                  aaaTCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCg                                                                                                   >  1:120256/1‑140 (MQ=255)                                    aTCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGtt                                                                                                 >  1:97706/1‑140 (MQ=255)                                      cAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCt                                                                                               >  2:309265/1‑140 (MQ=255)                                         aaaTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCGTATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCt                                                                                            <  1:267731/140‑1 (MQ=255)                                               aGCGTGAGGGGCACTCACGCTTTTGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTAttt                                                                                      >  2:942282/1‑140 (MQ=255)                                                          cACTCACGCTTTTGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCca                                                                           <  1:942282/140‑1 (MQ=255)                                                          cACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCca                                                                           <  2:120256/140‑1 (MQ=255)                                                          cACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCca                                                                           >  1:768846/1‑140 (MQ=255)                                                           aCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCcac                                                                          >  2:908320/1‑140 (MQ=255)                                                                cGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGAcc                                                                     <  2:97706/140‑1 (MQ=255)                                                                cGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGAcc                                                                     <  2:628408/140‑1 (MQ=255)                                                                 gCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCa                                                                    >  2:1041272/1‑140 (MQ=255)                                                                   tttCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACg                                                                  <  1:1041272/140‑1 (MQ=255)                                                                           aaaCAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATAc                                                          <  1:267534/140‑1 (MQ=255)                                                                             aCAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGAc                                                                      >  1:402344/1‑126 (MQ=255)                                                                             aCAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGAc                                                                      <  2:402344/126‑1 (MQ=255)                                                                             aCAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACca                                                        >  1:370986/1‑140 (MQ=255)                                                                                gTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACcatca                                                     <  2:768846/140‑1 (MQ=255)                                                                                 tAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATAc                                                          >  2:431310/1‑134 (MQ=255)                                                                                 tAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATAc                                                          <  1:295486/134‑1 (MQ=255)                                                                                 tAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATAc                                                          >  2:295486/1‑134 (MQ=255)                                                                                 tAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATAc                                                          <  1:431310/134‑1 (MQ=255)                                                                                 tAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACcatcat                                                    >  2:775972/1‑140 (MQ=255)                                                                                  aaaaTGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGt                                                                >  2:667921/1‑127 (MQ=255)                                                                                  aaaaTGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGt                                                                <  1:667921/127‑1 (MQ=255)                                                                                           gatgatAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTAATCAGGaa                                          >  1:815452/1‑140 (MQ=255)                                                                                                  aTTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTaa                                                 >  1:545813/1‑126 (MQ=255)                                                                                                  aTTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTaa                                                 <  2:545813/126‑1 (MQ=255)                                                                                                  aTTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTAATCAGGAAAATGAcc                                   <  2:370986/140‑1 (MQ=255)                                                                                                      cGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTAATCAGGAAAATGACCCCTg                               <  1:775972/140‑1 (MQ=255)                                                                                                           tttttATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTAATCAGGAAAATGACCCCTGTTTCg                          >  1:958266/1‑140 (MQ=255)                                                                                                            ttttATCTGTTAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCAGAGACCACGGTCGATACCATCATTAATCAGGAAAATGACCCCTGTTTCGa                         <  2:669350/140‑1 (MQ=255)                                                                                                                       aGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTAATCAGGAAAATTACCCCTGTTTCGATAGCCGACATc              >  2:619039/1‑140 (MQ=255)                                                                                                                               ggTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTAATCAGGAAAATGACCCCTGTTTCGATAGCCGACATCAGGCACAg      >  2:716377/1‑140 (MQ=255)                                                                                                                                   gAACCCGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTAATCAGGAAAATGACCCCTGTTTCGATAGCCGACATCAGGCACAGCATa  >  1:966247/1‑140 (MQ=255)                                                                                                                                     aCCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTAATCAGGAAAATGACCCCTGTTTCGATAGCCGACa                <  1:301129/124‑1 (MQ=255)                                                                                                                                     aCCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTAATCAGGAAAATGACCCCTGTTTCGATAGCCGACa                >  2:301129/1‑124 (MQ=255)                                                                                                                                     aCCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTAATCAGGAAAATGACCCCTGTTTCGATAGCCGACa                <  1:960231/124‑1 (MQ=255)                                                                                                                                     aCCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTAATCAGGAAAATGACCCCGGTTTCGATAGCCGACa                >  2:960231/1‑124 (MQ=255)                                                                                                                                         |                                                                                                                                      CGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGTCGATACCATCATTAATCAGGAAAATGACCCCTGTTTCGATAGCCGACATCAGGCACAGCATA  >  NC_000913/1100735‑1101004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑