Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,105,737	A→G	intergenic (+12/‑83)	ymdA → / → ymdB	uncharacterized protein YmdA/2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,105,737	0	A	G	100.0%	157.8 / NA	44	intergenic (+12/‑83)	ymdA/ymdB	uncharacterized protein YmdA/2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/22);  total (22/22)

GGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGAT  >  NC_000913/1105600‑1105873                                                                                                                                          |                                                                                                                                         ggCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCt                                                                                                                                        >  2:461451/1‑140 (MQ=255) ggCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCt                                                                                                                                        >  2:999665/1‑140 (MQ=255) ggCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCt                                                                                                                                        >  1:534056/1‑140 (MQ=255) ggCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCt                                                                                                                                        >  2:757507/1‑140 (MQ=255) ggCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCt                                                                                                                                        >  2:828065/1‑140 (MQ=255)      aaCCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGt                                                                                                                                   >  1:386249/1‑140 (MQ=255)           aCTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTg                                                                                                                              >  2:963590/1‑140 (MQ=255)                  cAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTg                                                                                                                                 <  1:139567/130‑1 (MQ=255)                  cAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTg                                                                                                                                 >  2:139567/1‑130 (MQ=255)                       cGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATg                                                                                                                  >  1:630872/1‑140 (MQ=255)                                 cGGCAATGTCAAAAACGGGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAAcc                                                                                                        >  2:991795/1‑140 (MQ=255)                                 cGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAAcc                                                                                                        >  1:51068/1‑140 (MQ=255)                                 cGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAAcc                                                                                                        >  1:582444/1‑140 (MQ=255)                                        gTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGc                                                                                                 <  1:757507/140‑1 (MQ=255)                                        gTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGc                                                                                                 <  1:999665/140‑1 (MQ=255)                                        gTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGc                                                                                                 <  2:934256/140‑1 (MQ=255)                                           aaaaaCGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTAACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCctg                                                                                              <  2:546202/140‑1 (MQ=255)                                               aCGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGt                                                                                          <  1:963590/140‑1 (MQ=255)                                                cgcgCAAATAGCCTCAGTAAAAGTGCAGTATTTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGATTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTa                                                                                         <  1:1056949/140‑1 (MQ=255)                                                 gcgcAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTac                                                                                        <  2:51068/140‑1 (MQ=255)                                                  cgcAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTaca                                                                                       >  1:211342/1‑140 (MQ=255)                                                         aGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGa                                                                                <  1:461451/140‑1 (MQ=255)                                                           ccTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGaaa                                                                              >  2:127032/1‑140 (MQ=255)                                                               aGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACaag                                                                          <  1:826597/140‑1 (MQ=255)                                                               aGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACaag                                                                          <  1:93539/140‑1 (MQ=255)                                                                gTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACaaga                                                                         <  1:127032/140‑1 (MQ=255)                                                                gTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACaaga                                                                         <  2:534056/140‑1 (MQ=255)                                                                gTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACaaga                                                                         <  1:991795/140‑1 (MQ=255)                                                                gTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACaaga                                                                         <  1:828065/140‑1 (MQ=255)                                                                     aGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGgtg                                                                    >  2:963407/1‑140 (MQ=255)                                                                         cAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACaaga                                                                         <  2:813434/131‑1 (MQ=255)                                                                         cAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACaaga                                                                         >  1:813434/1‑131 (MQ=255)                                                                                        cAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGaaaa                                                 >  1:480089/1‑140 (MQ=255)                                                                                                gctggctgTAATGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTAtt                                         <  2:480089/140‑1 (MQ=255)                                                                                                          aTGAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGc                               <  2:386249/140‑1 (MQ=255)                                                                                                            gAACATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAg                             <  1:963407/140‑1 (MQ=255)                                                                                                             aaCATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAgg                            <  2:630872/140‑1 (MQ=255)                                                                                                               cATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTtgtg                                <  2:550434/134‑1 (MQ=255)                                                                                                               cATCGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTtgtg                                >  1:550434/1‑134 (MQ=255)                                                                                                                  cGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGat                       >  2:252421/1‑140 (MQ=255)                                                                                                                  cGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGat                       >  2:932755/1‑140 (MQ=255)                                                                                                                  cGAATATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGat                       >  2:1018349/1‑140 (MQ=255)                                                                                                                    aaTATAACTAAGTTTCTGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGatat                     >  2:946469/1‑140 (MQ=255)                                                                                                                                    tGAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGtt     >  2:952679/1‑140 (MQ=255)                                                                                                                                     gAACAGCTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTg    <  1:152256/140‑1 (MQ=255)                                                                                                                                       acagcTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGAt  <  1:952679/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTGCAGTATCTTGATAAACAGAAAAAGCTGGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGAT  >  NC_000913/1105600‑1105873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑