Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	78,637	A→G	A339A (GCA→GCG)	setA →	sugar exporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	78,637	0	A	G	100.0%	179.4 / NA	50	A339A (GCA→GCG)	setA	sugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/29);  total (21/29)

TTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGT  >  NC_000913/78500‑78762                                                                                                                                          |                                                                                                                              ttttACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGggcggc                                                                                                                             >  1:748211/1‑140 (MQ=255)   ttACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTa                                                                                                                           <  1:967175/140‑1 (MQ=255)   ttACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTa                                                                                                                           <  2:1075940/140‑1 (MQ=255)   ttACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTa                                                                                                                           <  2:689103/140‑1 (MQ=255)         cGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTaccacc                                                                                                                      <  1:882277/139‑1 (MQ=255)         cGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTaccacc                                                                                                                      >  2:882277/1‑139 (MQ=255)         cGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCt                                                                                                                     <  1:1078174/140‑1 (MQ=255)           gattgattTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTacca                                                                                                                        >  1:210132/1‑135 (MQ=255)           gattgattTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTacca                                                                                                                        <  2:210132/135‑1 (MQ=255)                 ttttCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACt                                                                                                             <  1:85039/140‑1 (MQ=255)                    tCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAAc                                                                                                          <  2:681582/140‑1 (MQ=255)                    tCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAAc                                                                                                          <  1:580033/140‑1 (MQ=255)                     cTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCt                                                                                                                     >  1:180388/1‑128 (MQ=255)                     cTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCt                                                                                                                     <  2:180388/128‑1 (MQ=255)                     cTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACa                                                                                                         <  2:1035705/140‑1 (MQ=255)                      tttAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAg                                                                                                        <  1:516572/140‑1 (MQ=255)                          aTAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAAc                                                                                                          <  1:779783/134‑1 (MQ=255)                          aTAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAAc                                                                                                          >  2:779783/1‑134 (MQ=255)                               cGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTAc                                                                                               <  1:316945/140‑1 (MQ=255)                                    ggCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCgggg                                                                                          <  2:477757/140‑1 (MQ=255)                                    ggCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCgggg                                                                                          <  2:748211/140‑1 (MQ=255)                                           atgaCGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTGTTTCTACCGGGGTAATTct                                                                                   >  1:421977/1‑140 (MQ=255)                                           atgaCGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTct                                                                                   >  2:221165/1‑140 (MQ=255)                                              aCGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTctggc                                                                                <  1:754315/140‑1 (MQ=255)                                              aCGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTctggc                                                                                >  2:1024915/1‑140 (MQ=255)                                                   gCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCg                                                                           <  2:585927/140‑1 (MQ=255)                                                              aCGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGa                                                                >  1:16359/1‑140 (MQ=255)                                                                 cTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCa                                                             >  2:357451/1‑140 (MQ=255)                                                                    tATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAAtt                                                          >  1:1061573/1‑140 (MQ=255)                                                                            ggCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAg                                                  >  1:864513/1‑140 (MQ=255)                                                                              cATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGtt                                                >  1:333712/1‑140 (MQ=255)                                                                                   ttgCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTggggg                                           <  1:1024915/140‑1 (MQ=255)                                                                                     gCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCa                                         <  1:221165/140‑1 (MQ=255)                                                                                     gCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCa                                         <  1:38211/140‑1 (MQ=255)                                                                                     gCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCa                                         <  2:333712/140‑1 (MQ=255)                                                                                       gggTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACt                                       <  2:946277/140‑1 (MQ=255)                                                                                            ttGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCt                                  <  1:572100/140‑1 (MQ=255)                                                                                            ttGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCt                                  >  1:843423/1‑140 (MQ=255)                                                                                             tGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTCATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTg                                    >  1:704328/1‑137 (MQ=255)                                                                                             tGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTCATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTg                                    <  2:704328/137‑1 (MQ=255)                                                                                             tGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGG‑GGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGt                                >  2:132418/1‑140 (MQ=255)                                                                                                   gCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACt                           >  1:726865/1‑140 (MQ=255)                                                                                                    cTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCTCCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTg                          >  2:827873/1‑140 (MQ=255)                                                                                                    cTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTg                          >  1:705582/1‑140 (MQ=255)                                                                                                            tCAGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTg                  >  1:198331/1‑140 (MQ=255)                                                                                                              aGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCg                <  1:357451/140‑1 (MQ=255)                                                                                                              aGGATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCg                >  2:875892/1‑140 (MQ=255)                                                                                                               ggATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCg                >  1:799158/1‑139 (MQ=255)                                                                                                               ggATTTAATGCCTGGAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCg                <  2:799158/139‑1 (MQ=255)                                                                                                                            ggAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCtgttgt  <  2:198331/140‑1 (MQ=255)                                                                                                                            ggAAGAGCGGGGGCGGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCtgttgt  <  2:843423/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                              TTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGT  >  NC_000913/78500‑78762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑