Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,162,938	A→G	S101G (AGC→GGC)	lpoB →	outer membrane lipoprotein ‑ activator of MrcB activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,162,938	0	A	G	100.0%	109.7 / NA	33	S101G (AGC→GGC)	lpoB	outer membrane lipoprotein ‑ activator of MrcB activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/17);  total (16/17)

CCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTAGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCAACTGTCGATGGC  >  NC_000913/1162802‑1163074                                                                                                                                         |                                                                                                                                         cccGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCg                                                                                                                                       <  1:361279/140‑1 (MQ=255)        cagcCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCctgct                                                                                                                                >  2:593729/1‑140 (MQ=255)        cagcCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCctgct                                                                                                                                >  1:113894/1‑140 (MQ=255)           ccAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGt                                                                                                                             <  1:504798/140‑1 (MQ=255)             aGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTg                                                                                                                           <  1:969418/140‑1 (MQ=255)             aGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTg                                                                                                                           <  1:573893/140‑1 (MQ=255)                       gAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTaa                                                                                                                 <  1:780830/140‑1 (MQ=255)                        aGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTaac                                                                                                                <  1:226596/140‑1 (MQ=255)                        aGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTaac                                                                                                                <  2:113894/140‑1 (MQ=255)                        aGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTaac                                                                                                                >  2:620155/1‑140 (MQ=255)                        aGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTaac                                                                                                                <  1:50309/140‑1 (MQ=255)                                       cTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGt                                                                                                 >  1:135331/1‑140 (MQ=255)                                              gCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGa                                                                                          >  2:604958/1‑140 (MQ=255)                                               ccTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGaa                                                                                         <  1:620155/140‑1 (MQ=255)                                                             tCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGa                                                                           >  1:699288/1‑140 (MQ=255)                                                                  aTTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGaa                                                                      >  2:921917/1‑140 (MQ=255)                                                                       gACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCt                                                                 <  1:799665/140‑1 (MQ=255)                                                                           ggAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGa                                                             >  1:493099/1‑140 (MQ=255)                                                                                 gcgcAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATgcg                                                       <  1:926624/140‑1 (MQ=255)                                                                                 gcgcAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATgcg                                                       <  1:279469/140‑1 (MQ=255)                                                                                    cAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTg                                                    >  2:795399/1‑140 (MQ=255)                                                                                       tGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCt                                                 <  1:501671/140‑1 (MQ=255)                                                                                               aTGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACgg                                         >  2:606855/1‑140 (MQ=255)                                                                                                              cTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGt                          >  2:11010/1‑140 (MQ=255)                                                                                                              cTTGGGGCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGt                          <  2:135331/140‑1 (MQ=255)                                                                                                                 ggggCTGACGGGGTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTc                       >  2:939631/1‑140 (MQ=255)                                                                                                                          ggggTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCa              <  1:604958/140‑1 (MQ=255)                                                                                                                          ggggTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCa              <  1:593729/140‑1 (MQ=255)                                                                                                                             gTGACTGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCAACt           >  2:588379/1‑140 (MQ=255)                                                                                                                                  tGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCAACTGTCGa      >  2:3685/1‑140 (MQ=255)                                                                                                                                  tGCGGGTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCAACTGTCGa      >  1:840209/1‑140 (MQ=255)                                                                                                                                     gggTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCAACTGTCGATgg   <  2:840209/140‑1 (MQ=255)                                                                                                                                      ggTGGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCAACTGTCGATGGc  >  2:1045073/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         CCCGCAGCAGCCAGGCCCAATTGAGCACGAAGATCAAACTGCACCGCCTGCGCCGCATATTCGCCATTATGACTGGAATGGCGCAATGCAGCCGATGGTCAGTAAGATGCTTGGGGCTGACGGGGTGACTGCGGGTAGCGTCCTGCTGGTTGATAGCGTTAACAACCGTACTAACGGTTCGCTGAATGCCGCAGAAGCGACCGAAACGCTGCGAAATGCGCTGGCTAATAACGGGAAATTTACCCTGGTTTCCGCCCAGCAACTGTCGATGGC  >  NC_000913/1162802‑1163074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑