Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,175,955	C→T	R177C (CGT→TGT)	lolC →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,175,955	0	C	T	100.0%	109.6 / NA	34	R177C (CGT→TGT)	lolC	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/18);  total (16/18)

ATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTG  >  NC_000913/1175818‑1176091                                                                                                                                          |                                                                                                                                         aTGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGgtgt                                                                                                                                        >  2:117758/1‑140 (MQ=255) aTGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGgtgt                                                                                                                                        >  2:1018717/1‑140 (MQ=255)      aaacaaacTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTcc                                                                                                                                   <  2:434515/140‑1 (MQ=255)      aaacaaacTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTcc                                                                                                                                   <  1:253229/140‑1 (MQ=255)                         cGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAAt                                                                                                                <  1:1018717/140‑1 (MQ=255)                            ggAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGcc                                                                                                                              >  2:167801/1‑123 (MQ=255)                            ggAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGcc                                                                                                                              <  1:167801/123‑1 (MQ=255)                                     aTGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAAtg                                                                                                               <  2:912828/129‑1 (MQ=255)                                     aTGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAAtg                                                                                                               >  1:912828/1‑129 (MQ=255)                                      tGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACtt                                                                                                   >  2:8395/1‑140 (MQ=255)                                        tCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTc                                                                                                 >  2:673518/1‑140 (MQ=255)                                          aTCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTcgc                                                                                               >  1:836090/1‑140 (MQ=255)                                           tCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTcgcc                                                                                              >  2:1030150/1‑140 (MQ=255)                                           tCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGCTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTcgcc                                                                                              >  2:135421/1‑140 (MQ=255)                                             cTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTcgccgc                                                                                            <  2:836090/140‑1 (MQ=255)                                                ggCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCaa                                                                                         <  1:135421/140‑1 (MQ=255)                                                            gCCTCACAGCTGGGCTTTAATCGCGGTAATCAAACCCGCGCGATGGTACTATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGa                                                                             <  2:543673/140‑1 (MQ=255)                                                            gCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGa                                                                             <  2:836013/140‑1 (MQ=255)                                                               tCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATgg                                                                          <  2:225156/140‑1 (MQ=255)                                                                 acaGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCt                                                                        >  2:40510/1‑140 (MQ=255)                                                                             tAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGTCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTgg                                                            >  2:1010105/1‑140 (MQ=255)                                                                             tAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATg                                                                >  2:133713/1‑136 (MQ=255)                                                                             tAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATg                                                                <  1:133713/136‑1 (MQ=255)                                                                              aaTCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGt                                                           <  1:117758/140‑1 (MQ=255)                                                                               aTCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTg                                                          <  2:1081613/140‑1 (MQ=255)                                                                                   cgGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAata                                                      <  1:673518/140‑1 (MQ=255)                                                                                    ggTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAatat                                                     >  1:126841/1‑140 (MQ=255)                                                                                    ggTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAatat                                                     <  2:723284/140‑1 (MQ=255)                                                                                                       tGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTg                                  <  2:426486/140‑1 (MQ=255)                                                                                                               tCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCCAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTa                          >  2:6130/1‑140 (MQ=255)                                                                                                               tCTGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTa                          >  2:85773/1‑140 (MQ=255)                                                                                                                 tGCCAGCCAGTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATc                        >  1:1016239/1‑140 (MQ=255)                                                                                                                    cagccagTTCACGCCGATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCgg                     <  1:1030150/140‑1 (MQ=255)                                                                                                                                   gATGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCg      <  2:402684/140‑1 (MQ=255)                                                                                                                                     tGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGc    >  2:31918/1‑140 (MQ=255)                                                                                                                                     tGGGGTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGc    >  2:874887/1‑140 (MQ=255)                                                                                                                                       gggTGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTg  <  2:1016239/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTG  >  NC_000913/1175818‑1176091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑