Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,177,836	T→C	S173P (TCG→CCG)	lolE →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,177,836	0	T	C	100.0%	121.0 / NA	35	S173P (TCG→CCG)	lolE	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/18);  total (17/18)

CGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACTCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCGGATGCCCAACAATATCTTGATAT  >  NC_000913/1177700‑1177969                                                                                                                                         |                                                                                                                                      cgcATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGa                                                                                                                                    <  1:186706/140‑1 (MQ=255)    aTTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATc                                                                                                                                 >  2:905981/1‑140 (MQ=255)     ttACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATcc                                                                                                                                <  1:458514/140‑1 (MQ=255)      tACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCTGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATccc                                                                                                                               >  1:830087/1‑140 (MQ=255)      tACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAACGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATccc                                                                                                                               <  2:316262/140‑1 (MQ=255)      tACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATccc                                                                                                                               >  1:230539/1‑140 (MQ=255)         ccTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAg                                                                                                                            >  2:597431/1‑140 (MQ=255)             gTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATa                                                                                                                        >  1:233195/1‑140 (MQ=255)                  tCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATGCCGAGCATAAACTg                                                                                                                   >  2:1078755/1‑140 (MQ=255)                  tCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGc                                                                                                                           >  1:1073376/1‑132 (MQ=255)                  tCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGc                                                                                                                           <  2:1073376/132‑1 (MQ=255)                  tCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGGGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTg                                                                                                                   >  1:985147/1‑140 (MQ=255)                  tCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGATGTGAAAGAGAAGCATTGGGGTTGGGGGTCGAGTATGATCCCCAACCCGAATCCCGAGCATGAAcgg                                                                                                                   >  2:60499/1‑138 (MQ=255)                     aGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCTGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATg                                                                                                                <  2:830087/140‑1 (MQ=255)                                   cgcAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACgtgt                                                                                                  >  2:1007022/1‑140 (MQ=255)                                        ttttAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGtt                                                                                             <  1:828944/140‑1 (MQ=255)                                        ttttAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGtt                                                                                             <  1:685624/140‑1 (MQ=255)                                            aaaGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCa                                                                                         <  2:230539/140‑1 (MQ=255)                                                  ggCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGc                                                                                   <  2:985147/140‑1 (MQ=255)                                                  ggCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGc                                                                                   <  1:807454/140‑1 (MQ=255)                                                                   catcGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTTTGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGa                                                                  <  2:951243/140‑1 (MQ=255)                                                                   catcGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGa                                                                  <  2:134328/140‑1 (MQ=255)                                                                    atcGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAg                                                                 <  1:1007022/140‑1 (MQ=255)                                                                     tcGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTTTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGt                                                                >  2:748093/1‑140 (MQ=255)                                                                                 tGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGAt                                                    <  1:597431/140‑1 (MQ=255)                                                                                                     aaGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATccc                                >  1:976351/1‑140 (MQ=255)                                                                                                      aGCAGGGGGATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCg                               >  1:256655/1‑140 (MQ=255)                                                                                                           ggggATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGc                          >  1:985085/1‑140 (MQ=255)                                                                                                            gggATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCg                         >  1:541996/1‑140 (MQ=255)                                                                                                             ggATTGGGTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCgg                        <  2:256655/140‑1 (MQ=255)                                                                                                                   ggTGTCGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCTATGATCCCGCTGGCGGATGccc                  <  2:976351/140‑1 (MQ=255)                                                                                                                        cGATTATGATCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCGGATGCCcaacaa             >  1:490126/1‑140 (MQ=255)                                                                                                                                 tCCCCAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCGGATGCCCAACAATATCTTGat    <  1:1078755/140‑1 (MQ=255)                                                                                                                                   cccAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCGGATGCCCAACAATATCTTGatat  <  2:490126/140‑1 (MQ=255)                                                                                                                                   cccAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCGGATGCCCAACAATATCTTGatat  >  2:631904/1‑140 (MQ=255)                                                                                                                                   cccAACCCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTAGTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCGGATGCCCAACAATATCTTGatat  >  2:1009471/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGAAGCAGGGGGATTGGGTGTCGATTATGATCCCCAACTCGAATCCCGAGCATAAACTGATGCAGCCAAAACGTGTGCGTTTGCACGTTGCCGGTATTTTGCAGTTGAGTGGTCAACTCGATCACAGTTTTGCCATGATCCCGCTGGCGGATGCCCAACAATATCTTGATAT  >  NC_000913/1177700‑1177969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑