Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,336,633	T→C	V268A (GTT→GCT)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,336,633	0	T	C	100.0%	183.3 / NA	51	V268A (GTT→GCT)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/25);  total (26/25)

GCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCC  >  NC_000913/1336499‑1336766                                                                                                                                       |                                                                                                                                      gCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCa                                                                                                                                  <  1:626005/140‑1 (MQ=255) gCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCa                                                                                                                                  <  1:314624/140‑1 (MQ=255)  cTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCAc                                                                                                                                 >  1:445384/1‑140 (MQ=255)     gggtggggCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGt                                                                                                                              <  1:149976/140‑1 (MQ=255)          gggCGTTGGTGGGATCGAAGCAGTAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTAccc                                                                                                                         >  1:798103/1‑140 (MQ=255)          gggCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTAccc                                                                                                                         <  1:1005631/140‑1 (MQ=255)               ttGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAAtg                                                                                                                    >  2:409923/1‑140 (MQ=255)               ttGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAAtg                                                                                                                    <  1:880470/140‑1 (MQ=255)                tggtggGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAAtgc                                                                                                                   <  1:409923/140‑1 (MQ=255)                 ggtggGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCAc                                                                                                                                 >  2:593969/1‑125 (MQ=255)                 ggtggGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCAc                                                                                                                                 <  1:593969/125‑1 (MQ=255)                 ggtggGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAAtgct                                                                                                                  >  1:511414/1‑140 (MQ=255)                 ggtggGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAAtgct                                                                                                                  >  1:174238/1‑140 (MQ=255)                       aTCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCaa                                                                                                            <  2:445384/140‑1 (MQ=255)                               aGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCg                                                                                                    <  1:490295/140‑1 (MQ=255)                                 aaGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCgtg                                                                                                  >  2:637506/1‑140 (MQ=255)                                 aaGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCgtg                                                                                                  >  2:1066510/1‑140 (MQ=255)                                 aaGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAAATTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCgtg                                                                                                  >  2:751159/1‑140 (MQ=255)                                                    gCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAAt                                                                               >  2:6012/1‑140 (MQ=255)                                                               tGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGAt                                                                    >  2:1075596/1‑140 (MQ=255)                                                                    aTCCGGGATGTAGTGGGCTTCAATCTTCCCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCt                                                               <  1:960831/140‑1 (MQ=255)                                                                     tCCCGGATGTAGTGGGCTTCAACCTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTg                                                              >  1:138603/1‑140 (MQ=255)                                                                     tCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTg                                                                      >  2:6854/1‑132 (MQ=255)                                                                     tCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTg                                                                      <  1:6854/132‑1 (MQ=255)                                                                     tCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTg                                                              >  2:845657/1‑140 (MQ=255)                                                                      cccGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTgg                                                             <  2:690728/140‑1 (MQ=255)                                                                         ggATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGAtt                                                          <  1:427706/140‑1 (MQ=255)                                                                          gATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTg                                                                      >  2:1037953/1‑127 (MQ=255)                                                                          gATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTg                                                                      <  1:1037953/127‑1 (MQ=255)                                                                          gATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTc                                                         >  2:689373/1‑140 (MQ=255)                                                                           aTGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGAt                                                           <  2:1010668/137‑1 (MQ=255)                                                                           aTGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGAt                                                           >  1:1010668/1‑137 (MQ=255)                                                                           aTGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCa                                                        <  1:751159/140‑1 (MQ=255)                                                                           aTGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCa                                                        <  1:1066510/140‑1 (MQ=255)                                                                               aGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTAc                                                    >  1:193244/1‑140 (MQ=255)                                                                                gTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTAcc                                                   >  2:61424/1‑140 (MQ=255)                                                                                     ctcaaaaCTAACCGAAAAATTACGTGAAGAAATTTCCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTgg                                              <  2:511414/136‑1 (MQ=255)                                                                                     cTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTgg                                              >  1:762699/1‑140 (MQ=255)                                                                                     cTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCCGGATTCACTACCGTTgg                                              >  2:343061/1‑140 (MQ=255)                                                                                               aCCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCgcgc                                    >  1:589572/1‑140 (MQ=255)                                                                                               aCCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCgcgc                                    >  1:289433/1‑140 (MQ=255)                                                                                                    aaaaTTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGccacca                               <  1:845657/140‑1 (MQ=255)                                                                                                    aaaaTTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCCGGATTCACTACCGTTGGCGGATCGCGccacca                               <  1:343061/140‑1 (MQ=255)                                                                                                     aaaTTACGTGAAGGTATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCAt                              <  1:1075596/140‑1 (MQ=255)                                                                                                              gAAGGTTTTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAatat                     <  2:989435/140‑1 (MQ=255)                                                                                                                   tATTACCGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGc                <  2:138603/140‑1 (MQ=255)                                                                                                                         cGCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAat          <  2:589572/140‑1 (MQ=255)                                                                                                                          gCCACAGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAata         >  1:360153/1‑140 (MQ=255)                                                                                                                            cacaGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATg       >  1:417621/1‑140 (MQ=255)                                                                                                                            cacaGACCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATg       >  1:294568/1‑140 (MQ=255)                                                                                                                                 aCCTGGCTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGcc  <  2:193244/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      GCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCC  >  NC_000913/1336499‑1336766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑