Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,338,923	C→T	E80K (GAG→AAG)	ribA ←	GTP cyclohydrolase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,338,923	0	C	T	100.0%	181.7 / NA	52	E80K (GAG→AAG)	ribA	GTP cyclohydrolase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/25);  total (27/25)

CGAAGCCTAACTGGTGGTTAGCCTCTACGGTATCGTAACCTTGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTCGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTACCGGGGTATGCCCGGAAATATCGCCATAGA  >  NC_000913/1338791‑1339054                                                                                                                                     |                                                                                                                                    cgAAGCCTAACTGGTGGTTAGCCTCTACGGTATCGTAACCTTGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGGCCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAAtt                                                                                                                              >  1:50513/1‑140 (MQ=255)   aaGCCTAACTGGTGGTTAGCCTCTACGGTATCGTAACCTTGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTg                                                                                                                            >  2:610509/1‑140 (MQ=255)                        tctACGGTATCGTAACCTTGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTg                                                                                                       <  1:337689/140‑1 (MQ=255)                         ctACGGTATCGTAACCTTGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTgg                                                                                                      <  1:612651/140‑1 (MQ=255)                          tACGGTATCGTAACCTTGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGa                                                                                                     >  2:1053610/1‑140 (MQ=255)                           aCGGTATCGTAACCTTGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCtt                                                                                                              <  1:344098/130‑1 (MQ=255)                           aCGGTATCGTAACCTTGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCtt                                                                                                              >  2:344098/1‑130 (MQ=255)                            cGGTATCGTAACCTTGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAg                                                                                                   >  2:881208/1‑140 (MQ=255)                                    tAACCTTGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATc                                                                                           <  1:881208/140‑1 (MQ=255)                                    tAACCTTGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATc                                                                                           <  1:610509/140‑1 (MQ=255)                                     aaCCTTGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCg                                                                                          <  2:426559/140‑1 (MQ=255)                                         ttGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAgc                                                                                      <  1:1053610/140‑1 (MQ=255)                                         ttGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAgc                                                                                      <  2:919617/140‑1 (MQ=255)                                           gATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAgcgc                                                                                    >  1:953918/1‑140 (MQ=255)                                           gATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAgcgc                                                                                    >  2:488843/1‑140 (MQ=255)                                           gATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAgcgc                                                                                    >  1:166216/1‑140 (MQ=255)                                              ccTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGTAAGCCACAATCGCAGCGCAAg                                                                                 <  1:466619/140‑1 (MQ=255)                                               cTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGc                                                                                <  2:953918/140‑1 (MQ=255)                                                       cGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAgc                                                                                      <  2:927935/126‑1 (MQ=255)                                                       cGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAgc                                                                                      >  1:927935/1‑126 (MQ=255)                                                         tAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGc                                                                      >  1:523773/1‑140 (MQ=255)                                                              gcgGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCAc                                                                 <  2:502370/140‑1 (MQ=255)                                                               cgGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCAcc                                                                >  1:530602/1‑140 (MQ=255)                                                                 gATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCgg                                                              >  2:1071067/1‑140 (MQ=255)                                                                    tttATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCa                                                           <  2:523773/140‑1 (MQ=255)                                                                    tttATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCa                                                           <  2:530602/140‑1 (MQ=255)                                                                    tttATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCa                                                           <  2:166216/140‑1 (MQ=255)                                                                             cagACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCgg                                                  >  2:486479/1‑140 (MQ=255)                                                                                aCCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAAt                                               >  2:817103/1‑140 (MQ=255)                                                                                aCCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAAt                                               >  1:742853/1‑140 (MQ=255)                                                                                         aCGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGAcgcgcg                                      <  1:486479/140‑1 (MQ=255)                                                                                          cGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACgcgcgc                                     >  1:55331/1‑140 (MQ=255)                                                                                          cGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACgcgcgc                                     >  1:554734/1‑140 (MQ=255)                                                                                           gACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCa                                    <  2:421173/140‑1 (MQ=255)                                                                                           gACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCa                                    <  2:55331/140‑1 (MQ=255)                                                                                               ttCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTa                                >  1:443632/1‑140 (MQ=255)                                                                                               ttCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTa                                >  1:678952/1‑140 (MQ=255)                                                                                                tCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATTGACgcgcgc                                     >  1:155072/1‑134 (MQ=255)                                                                                                tCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATTGACgcgcgc                                     <  2:155072/134‑1 (MQ=255)                                                                                                tCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTAc                               <  1:817103/140‑1 (MQ=255)                                                                                                     aCGGTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTACCgggg                          <  1:488843/140‑1 (MQ=255)                                                                                                       ggTGATACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTACCGGGGTa                        >  2:1070387/1‑140 (MQ=255)                                                                                                           aTACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTACCGGGGTATGcc                    <  2:443632/140‑1 (MQ=255)                                                                                                            tACAGCAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTACCGGGGTATGccc                   >  2:803664/1‑140 (MQ=255)                                                                                                                 cAAAATACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTACCGGGGTATGCCCTGaaa              >  1:907891/1‑140 (MQ=255)                                                                                                                  aaaaTACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTACCGGGGTATGCCCGGAAat             >  1:552695/1‑140 (MQ=255)                                                                                                                   aaaTACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTACCGGGGTATGCCCGGAAata            >  1:8134/1‑140 (MQ=255)                                                                                                                      tACCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTACCGGGGTATGCCCGGAAATATCg         >  2:278668/1‑140 (MQ=255)                                                                                                                       aCCACGGCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTACCGGGGTATGCCCGGAAATATCGc        <  2:8134/140‑1 (MQ=255)                                                                                                                             gCCTTCCTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTACCGGGGTATGCCCGGAAATATCGCCATAGa  <  1:803664/140‑1 (MQ=255)                                                                                                                                  ccTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCACAAGTACCGGGGTATGCCCGGAAatat           <  1:1025616/126‑1 (MQ=255)                                                                                                                                  ccTTGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCACAAGTACCGGGGTATGCCCGGAAatat           >  2:1025616/1‑126 (MQ=255)                                                                                                                                     |                                                                                                                                    CGAAGCCTAACTGGTGGTTAGCCTCTACGGTATCGTAACCTTGATCCTGCAGTGCGTAAGCGCGGATTTTATTCAGCAGACCAATGTTACGACCTTCCTGACGGTGATACAGCAAAATACCACGGCCTTCCTCGGCAATTTGCGTCAATGCCGCTTCGAGCTGGAAGCCACAATCGCAGCGCAAGCTGAACAGGGCGTCACCGGTCAGACATTCGGAATGGACGCGCGCAAGTACCGGGGTATGCCCGGAAATATCGCCATAGA  >  NC_000913/1338791‑1339054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑