| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 213,286 | A→G | N319S (AAC→AGC) | tilS → | tRNA(Ile)‑lysidine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 213,286 | 0 | A | G | 97.8% | 157.0 / ‑4.2 | 46 | N319S (AAC→AGC) | tilS | tRNA(Ile)‑lysidine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (21/24); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
CGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGA > NC_000913/213151‑213422 | cgTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTAGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCAtt > 2:1009168/1‑140 (MQ=255) cTGGCAGGAAGTGGCGCTGCCGCCGGACGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGT‑GCAGAc < 2:758817/140‑1 (MQ=255) tGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGAc < 1:1009168/140‑1 (MQ=255) tGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGAc < 2:714737/140‑1 (MQ=255) ggCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACg > 2:1009591/1‑140 (MQ=255) ggCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACg > 1:612950/1‑140 (MQ=255) aGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTc < 1:1009591/140‑1 (MQ=255) aGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTc < 2:831821/140‑1 (MQ=255) aGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTc > 1:933600/1‑140 (MQ=255) tggcgctggcgcGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGAc < 2:229383/130‑1 (MQ=255) tggcgctggcgcGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGAc > 1:229383/1‑130 (MQ=255) ggcgctggcgcGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAAc < 1:1026493/140‑1 (MQ=255) ggcgcGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCaa > 2:638739/1‑133 (MQ=255) ggcgcGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCaa < 1:638739/133‑1 (MQ=255) ggcgcGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTgg > 1:424435/1‑140 (MQ=255) ggcgcGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTgg > 1:252356/1‑140 (MQ=255) aTGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCGCCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACcggcg < 2:969496/140‑1 (MQ=255) aTGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACcggcg < 2:303950/140‑1 (MQ=255) aTGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACcggcg < 2:252356/140‑1 (MQ=255) ccTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCgggg < 2:424435/140‑1 (MQ=255) tCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCt < 2:612950/140‑1 (MQ=255) aCCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTgg < 1:626195/140‑1 (MQ=255) cccTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTggg < 1:84085/140‑1 (MQ=255) cccTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTggg > 1:115240/1‑140 (MQ=255) cccTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTggg < 1:441460/140‑1 (MQ=255) cccTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGAGGCTTCAACCGCTGGAATTACCGGCGGGCCgggg > 2:725232/1‑140 (MQ=255) cTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGaa < 2:115240/140‑1 (MQ=255) gTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAg < 1:877708/140‑1 (MQ=255) tttGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGc > 1:183827/1‑140 (MQ=255) tGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACaacct > 2:82926/1‑136 (MQ=255) ggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTaa > 1:351671/1‑140 (MQ=255) ggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGAAGGGTAGAGCttta > 2:855244/1‑138 (MQ=255) ggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAGGGTAAAGCCTaa > 2:538773/1‑140 (MQ=255) gTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCgg < 1:725232/140‑1 (MQ=255) aaaTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGc < 1:828800/140‑1 (MQ=255) aaaTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGc < 1:538773/140‑1 (MQ=255) ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCGCCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGata < 1:855244/140‑1 (MQ=255) tCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCcgcg < 1:1080882/140‑1 (MQ=255) tCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCcgcg < 2:183827/140‑1 (MQ=255) tggtggATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTAATGCGGGAGGCGATATTTGCCCCCCCCGGGCCGAAGa > 1:392688/1‑140 (MQ=255) gtggATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCGGGGAAGGGTACAGCCTAATGCGGGGGGGGGGATTCGCCCCCCCCGTGCAGACGaaa > 2:62565/1‑139 (MQ=255) gATTAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTAATGCGGGGGGGGGTATTCGCCCCCCCCGTGCAGACGAAACgg > 2:453500/1‑140 (MQ=255) ttAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGGGATATTTGCCCTCCGCGGGCAGACGAAGCgggc > 2:231293/1‑138 (MQ=255) tAAATCCGTCACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGAAAGCTTAATGCGGGGGGGGGTATTTGCCCCACCCGGGCAGACGCAACGGTCa > 2:988663/1‑140 (MQ=255) cACCGGGCAAAGCGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTACAGCTTAATGCGGGGGGCGATATTTGCCCCCCCCGTGCAGACGAAGCGGGCAgcgtgcggt > 2:577834/1‑138 (MQ=255) cGAAAGCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAg < 2:614188/140‑1 (MQ=255) aaagCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGa < 1:390312/140‑1 (MQ=255) | CGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGA > NC_000913/213151‑213422 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |