Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,398,452	A→G	R57R (CGT→CGC)	uspE ←	universal stress protein with a role cellular motility

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,398,452	0	A	G	100.0%	213.4 / NA	60	R57R (CGT→CGC)	uspE	universal stress protein with a role cellular motility
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/26);  total (34/26)

AATGATGGCTTCGAAAGGACGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCTGGTTGGTCGTCCTGGTTAGG  >  NC_000913/1398320‑1398586                                                                                                                                     |                                                                                                                                         aaTGATGGCTTCGAAAGGACGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGt                                                                                                                                   <  1:253533/140‑1 (MQ=255) aaTGATGGCTTCGAAAGGACGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGt                                                                                                                                   <  1:505814/140‑1 (MQ=255)     atgGCTTCGAAAGGACGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCgg                                                                                                                               >  1:896829/1‑140 (MQ=255)     atgGCTTCGAAAGGACGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCgg                                                                                                                               >  2:512622/1‑140 (MQ=255)                aGGACGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAggg                                                                                                                    <  1:613237/140‑1 (MQ=255)                      gTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCa                                                                                                              <  1:592924/140‑1 (MQ=255)                               gACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATg                                                                                                     >  1:181498/1‑140 (MQ=255)                                aCCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATga                                                                                                    >  1:719933/1‑140 (MQ=255)                                 ccACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATgag                                                                                                   <  2:20246/140‑1 (MQ=255)                                 ccACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATgag                                                                                                   >  2:440805/1‑140 (MQ=255)                                 ccACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATgag                                                                                                   >  1:828338/1‑140 (MQ=255)                                 ccACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATgag                                                                                                   <  1:512622/140‑1 (MQ=255)                                 ccACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATgag                                                                                                   <  2:719933/140‑1 (MQ=255)                                 ccACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATgag                                                                                                   <  2:896829/140‑1 (MQ=255)                                   aCTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGaa                                                                                                 <  2:321117/140‑1 (MQ=255)                                    cTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAg                                                                                                >  2:748308/1‑140 (MQ=255)                                     tttAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCa                                                                                                              >  2:695393/1‑125 (MQ=255)                                     tttAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCa                                                                                                              <  1:695393/125‑1 (MQ=255)                                     tttAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGt                                                                                               >  2:632905/1‑140 (MQ=255)                                               aTGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑Tctc                                                                                   >  1:162857/1‑138 (MQ=255)                                                   gAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCaaa                                                                               <  2:828338/140‑1 (MQ=255)                                                        ccAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGc                                                                          >  2:910912/1‑140 (MQ=255)                                                              ttGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAAt                                                                    >  2:142048/1‑140 (MQ=255)                                                                      atatTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGccac                                                            <  1:1052372/140‑1 (MQ=255)                                                                           ttGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATc                                                       >  2:845756/1‑140 (MQ=255)                                                                            tGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGc                                                               >  2:273023/1‑131 (MQ=255)                                                                            tGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGc                                                               <  1:273023/131‑1 (MQ=255)                                                                             gCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCg                                                     <  1:440805/140‑1 (MQ=255)                                                                             gCCTGCTCGTGGATCCAGGCTGTACGCCGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCAGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCg                                                     >  1:453563/1‑140 (MQ=255)                                                                                 gCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGccacca                                                          >  1:635642/1‑131 (MQ=255)                                                                                 gCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGccacca                                                          <  2:635642/131‑1 (MQ=255)                                                                                  cTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGAt                                                >  1:633106/1‑140 (MQ=255)                                                                                  cTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGAt                                                >  2:1058855/1‑140 (MQ=255)                                                                                   tCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGATCTCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGa                                                 <  2:162857/140‑1 (MQ=255)                                                                                             aGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACa                                     >  1:995298/1‑140 (MQ=255)                                                                                               gCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGAGGTCATTTCGTATGAGAGGTCATAGA‑‑TAGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGc                                   >  2:971224/1‑140 (MQ=255)                                                                                                   tACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCGTTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTcgc                               >  1:726415/1‑140 (MQ=255)                                                                                                   tACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTcgc                               >  1:263698/1‑140 (MQ=255)                                                                                                   tACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTcgc                               >  2:828331/1‑140 (MQ=255)                                                                                                   tACGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTcgc                               >  1:526501/1‑140 (MQ=255)                                                                                                    aCGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTcgcc                              >  2:1048505/1‑140 (MQ=255)                                                                                                    aCGCTGGCTGATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTcgcc                              >  1:899744/1‑140 (MQ=255)                                                                                                        tggctgATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCaa                          >  1:473618/1‑140 (MQ=255)                                                                                                        tggctgATGACGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCaa                          >  1:303735/1‑140 (MQ=255)                                                                                                             gatgaCGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCtg                     >  1:29853/1‑140 (MQ=255)                                                                                                              atgaCGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCtgg                    <  2:633106/140‑1 (MQ=255)                                                                                                                gaCGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCtggtt                  <  1:673730/140‑1 (MQ=255)                                                                                                                  cGCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCtggttgg                <  1:1058855/140‑1 (MQ=255)                                                                                                                   gCCCTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCtggttggt               <  2:899744/140‑1 (MQ=255)                                                                                                                    cccTGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCTGGTTGgtc              >  2:36709/1‑140 (MQ=255)                                                                                                                       tGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCTGGTTGgtcgtc           <  2:526501/140‑1 (MQ=255)                                                                                                                       tGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCTGGTTGgtcgtc           <  2:473618/140‑1 (MQ=255)                                                                                                                       tGACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCTGGTTGgtcgtc           <  2:263698/140‑1 (MQ=255)                                                                                                                        gACGCATAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCTGGTTGGTCGTcc          >  1:230032/1‑140 (MQ=255)                                                                                                                             aTAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCtggttgg                <  1:442677/129‑1 (MQ=255)                                                                                                                             aTAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCtggttgg                >  2:442677/1‑129 (MQ=255)                                                                                                                              tAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCtggttgg                >  2:1017720/1‑128 (MQ=255)                                                                                                                              tAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCtggttgg                <  1:1017720/128‑1 (MQ=255)                                                                                                                              tAGCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCTGGTTGGTCGTCCTGGTTa    >  2:836483/1‑140 (MQ=255)                                                                                                                                gCGGTGCGTTCGTCCGGGGAGAGCAGGGTGGTCGTTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCTGGTTGGTCGTCCTGGTTAgg  <  2:726415/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         AATGATGGCTTCGAAAGGACGGTTATGCCAGACCACTTTAATTTCAATGGGAACGCCAGCATTGAGATAATATTTTGCCTGCTCGTGGATCCAGGCTGTACGCTGGCTGATGACGCCCTGACGCATAGCGGTACGTTCGTCCGGGGAGAGCAGGGTGGTCATTTCGTATGAGAAGTCATAGA‑‑TCGGCAAAAAGGCTTTAATTTTGCCACCAATCCGTTGATGTAAATAAACAGCTCGCCGCAATGCTGGTTGGTCGTCCTGGTTAGG  >  NC_000913/1398320‑1398586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑