Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,479,590	A→G	A151A (GCA→GCG)	ynbC →	hydrolase/methyltransferase domain‑containing protein YnbC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,479,590	0	A	G	100.0%	203.0 / NA	57	A151A (GCA→GCG)	ynbC	hydrolase/methyltransferase domain‑containing protein YnbC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/28);  total (29/28)

GGTGGTAGTGATCGCGCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATT  >  NC_000913/1479461‑1479725                                                                                                                                  |                                                                                                                                        ggtggtAGTGATCGCGCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTgcgc                                                                                                                               >  1:559548/1‑140 (MQ=255)        gTGATCGCGCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGtt                                                                                                                        <  2:559548/140‑1 (MQ=255)           aTCGCGCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATg                                                                                                                     >  1:668403/1‑140 (MQ=255)            tCGCGCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATgg                                                                                                                    >  2:814640/1‑140 (MQ=255)             cgcgCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGc                                                                                                                   <  2:730725/140‑1 (MQ=255)             cgcgCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGc                                                                                                                   >  1:431193/1‑140 (MQ=255)               cgcAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGtt                                                                                                                        >  1:1068443/1‑133 (MQ=255)               cgcAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGtt                                                                                                                        <  2:1068443/133‑1 (MQ=255)               cgcAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGtt                                                                                                                        >  2:628932/1‑133 (MQ=255)               cgcAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGtt                                                                                                                        <  1:628932/133‑1 (MQ=255)                gcAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATc                                                                                                                >  1:629834/1‑140 (MQ=255)                     gCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTg                                                                                                           >  2:633069/1‑140 (MQ=255)                      cGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGc                                                                                                          <  1:3688/140‑1 (MQ=255)                       gTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCg                                                                                                         <  1:491646/140‑1 (MQ=255)                           gcgcAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGt                                                                                                     <  1:1029500/140‑1 (MQ=255)                                       ttGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTttatt                                                                                         <  1:894028/140‑1 (MQ=255)                                         gCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTaa                                                                                       <  1:747024/140‑1 (MQ=255)                                           cacaTGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAAtt                                                                                     >  1:339792/1‑140 (MQ=255)                                           cacaTGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAAtt                                                                                     >  2:1046584/1‑140 (MQ=255)                                               tGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTa                                                                                 >  2:29019/1‑140 (MQ=255)                                                     cATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGaa                                                                           <  2:227680/140‑1 (MQ=255)                                                     cATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGaa                                                                           <  1:633069/140‑1 (MQ=255)                                                     cATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGaa                                                                           <  1:896203/140‑1 (MQ=255)                                                         aTTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGa                                                                       <  1:814640/140‑1 (MQ=255)                                                         aTTATGCACCGGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGa                                                                       >  2:571869/1‑140 (MQ=255)                                                            aTGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGa                                                                       >  1:75048/1‑137 (MQ=255)                                                            aTGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGa                                                                       <  2:75048/137‑1 (MQ=255)                                                            aTGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGc                                                                    >  2:791463/1‑140 (MQ=255)                                                                   tGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGa                                                             <  1:791463/140‑1 (MQ=255)                                                                    gCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACg                                                                     >  1:272261/1‑131 (MQ=255)                                                                    gCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACg                                                                     <  2:272261/131‑1 (MQ=255)                                                                    gCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGAc                                                            >  1:698713/1‑140 (MQ=255)                                                                    gCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGAc                                                            >  1:800651/1‑140 (MQ=255)                                                                     cAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGAcc                                                           >  1:70561/1‑140 (MQ=255)                                                                       aTTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCa                                                         >  1:211712/1‑140 (MQ=255)                                                                       aTTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCa                                                         <  1:571869/140‑1 (MQ=255)                                                                           gcgGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGAt                                                     <  1:821883/140‑1 (MQ=255)                                                                                   gTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACg                                             >  2:963204/1‑140 (MQ=255)                                                                                        ggCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACgggggg                                        <  2:698713/140‑1 (MQ=255)                                                                                         gCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGc                                       >  2:498346/1‑140 (MQ=255)                                                                                          cTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCg                                      <  2:431193/140‑1 (MQ=255)                                                                                             ctcCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGt                                   <  1:366901/140‑1 (MQ=255)                                                                                              tcCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGtt                                  >  2:927774/1‑140 (MQ=255)                                                                                               ccGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGttt                                 <  2:1037515/140‑1 (MQ=255)                                                                                                cGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTc                                <  1:1046584/140‑1 (MQ=255)                                                                                                  gCCTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCaa                              <  1:29019/140‑1 (MQ=255)                                                                                                   ccTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCaat                             >  2:1074884/1‑140 (MQ=255)                                                                                                    cTTTAAGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCaata                            <  1:1074884/140‑1 (MQ=255)                                                                                                        aaGGTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGa                        >  2:453555/1‑140 (MQ=255)                                                                                                          ggTTAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATc                      <  1:498346/140‑1 (MQ=255)                                                                                                             tAAATTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGc                   >  2:342124/1‑140 (MQ=255)                                                                                                              aaaTTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCt                  <  2:629834/140‑1 (MQ=255)                                                                                                              aaaTTGTATGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCt                  >  2:843082/1‑140 (MQ=255)                                                                                                                     aTGTGCCGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATcaca           >  1:654596/1‑140 (MQ=255)                                                                                                                           cGCTGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCg     >  2:510731/1‑140 (MQ=255)                                                                                                                              tGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGAtt  <  2:70561/140‑1 (MQ=255)                                                                                                                              tGGCGCGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGAtt  >  1:27295/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        GGTGGTAGTGATCGCGCAAAGCGTCGGCGCAGTGCTGGTTGCCACATGGATTCATGATTATGCACCTGCAATTCGCGGGCTGGTGCTGGCTTCTCCGGCCTTTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATT  >  NC_000913/1479461‑1479725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑