| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,646,191 | A→G | T99A (ACC→GCC) | ydfV → | Qin prophage; protein YdfV |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,646,191 | 0 | A | G | 97.1% | 110.3 / ‑4.3 | 34 | T99A (ACC→GCC) | ydfV | Qin prophage; protein YdfV |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (19/14); total (20/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CGGCCATCATCTTACTGGTACAGACACCATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGACCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAAT > NC_000913/1646055‑1646317 | cGGCCATCATCTTACTGGTACAGACACCATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTgacctggcct < 2:364858/140‑1 (MQ=255) catcTTACTGGTACAGACACCATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATa < 2:1064801/140‑1 (MQ=255) acacCATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGt > 1:790345/1‑140 (MQ=255) acCATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAgg < 2:258802/126‑1 (MQ=255) acCATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAgg > 1:258802/1‑126 (MQ=255) acCATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGttt > 2:931844/1‑140 (MQ=255) cATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAac < 2:985715/132‑1 (MQ=255) cATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAac > 1:985715/1‑132 (MQ=255) cATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGtt < 2:847279/137‑1 (MQ=255) cATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGtt > 1:847279/1‑137 (MQ=255) cATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTa > 2:471267/1‑140 (MQ=255) tataCTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATg < 2:454495/140‑1 (MQ=255) taCTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCa > 1:485953/1‑140 (MQ=255) ttGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGACCTGTCGATAAAGGAACGTAACACGTTTTATGCAacac > 1:174454/1‑140 (MQ=255) gtgACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATg < 2:61565/132‑1 (MQ=255) gtgACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATg > 1:61565/1‑132 (MQ=255) gtgACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACAccc > 1:970672/1‑140 (MQ=255) gACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGc > 1:1050734/1‑140 (MQ=255) tGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAattat > 1:62744/1‑140 (MQ=255) cacaACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTg > 1:187252/1‑140 (MQ=255) cacaACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTg > 1:61104/1‑140 (MQ=255) aactcaactTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCg < 2:982937/140‑1 (MQ=255) actcaactTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCGa > 1:363414/1‑140 (MQ=255) actcaactTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCGa > 2:564368/1‑140 (MQ=255) ctcaactTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCGaa > 2:185424/1‑140 (MQ=255) caactTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCGa < 2:159665/137‑1 (MQ=255) caactTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCGa > 1:159665/1‑137 (MQ=255) ttCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCGAACGTTTAccc < 1:564368/140‑1 (MQ=255) tCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGcc < 1:383775/124‑1 (MQ=255) tCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGcc > 2:383775/1‑124 (MQ=255) tttGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCGAACGTTTAc > 1:139086/1‑127 (MQ=255) tttGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCGAACGTTTAc < 2:139086/127‑1 (MQ=255) cTTTCTCGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAg > 1:607834/1‑140 (MQ=255) tctcGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGccc < 2:607834/140‑1 (MQ=255) tctcGTCGGCGGGTGAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGccc < 2:62744/140‑1 (MQ=255) gAGCGGTGACCTGGCCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAAt < 2:310742/140‑1 (MQ=255) | CGGCCATCATCTTACTGGTACAGACACCATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGACCTGTCGATAAAGGAACGTAACACGTTTTATGCAACACCCGCATGCGGCAGAAAATTATTGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAAT > NC_000913/1646055‑1646317 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |