Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	236,613	A→G	T183A (ACG→GCG)	dnaQ →	DNA polymerase III subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	236,613	0	A	G	100.0%	298.2 / NA	82	T183A (ACG→GCG)	dnaQ	DNA polymerase III subunit epsilon
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/38);  total (44/38)

TCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCATGAAGC  >  NC_000913/236480‑236746                                                                                                                                      |                                                                                                                                      tCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGa                                                                                                                                 <  1:302162/140‑1 (MQ=255)  cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGAt                                                                                                                                >  2:1040670/1‑140 (MQ=255)           cgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGc                                                                                                                       >  2:597324/1‑140 (MQ=255)             cAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGa                                                                                                                     <  2:864836/140‑1 (MQ=255)                   ccTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAg                                                                                                               >  1:297116/1‑140 (MQ=255)                    cTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAgg                                                                                                              <  2:297116/140‑1 (MQ=255)                         tGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGgagaga                                                                                                         <  1:665581/140‑1 (MQ=255)                          gCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGac                                                                                                        <  1:785254/140‑1 (MQ=255)                             ttATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGAcaca                                                                                                     >  1:1027138/1‑140 (MQ=255)                               aTGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACacaac                                                                                                   >  1:949713/1‑140 (MQ=255)                                   gCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGca                                                                                               >  1:842550/1‑140 (MQ=255)                                     tCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGcaac                                                                                             >  2:51712/1‑140 (MQ=255)                                     tCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGcaac                                                                                             <  2:949713/140‑1 (MQ=255)                                      cGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGcaaca                                                                                            >  1:230693/1‑140 (MQ=255)                                       gCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGgagaga                                                                                                         <  1:119981/126‑1 (MQ=255)                                       gCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGgagaga                                                                                                         >  2:119981/1‑126 (MQ=255)                                       gCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGcaacaa                                                                                           <  2:1027138/140‑1 (MQ=255)                                       gCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGcaacaa                                                                                           >  1:398524/1‑140 (MQ=255)                                       gCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGcaacaa                                                                                           <  1:896701/140‑1 (MQ=255)                                        cTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAg                                                                                          >  2:496746/1‑140 (MQ=255)                                         tACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAgg                                                                                         >  1:290736/1‑140 (MQ=255)                                          aCGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGt                                                                                        >  2:218672/1‑140 (MQ=255)                                               aTAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGc                                                                                   <  1:1040670/140‑1 (MQ=255)                                                      aCAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAAtt                                                                            <  2:230693/140‑1 (MQ=255)                                                      aCAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAAtt                                                                            <  2:719739/140‑1 (MQ=255)                                                         gTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAg                                                                         <  2:1051912/140‑1 (MQ=255)                                                          tAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGcaa                                                                                 >  1:1077371/1‑131 (MQ=255)                                                          tAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGcaa                                                                                 <  2:1077371/131‑1 (MQ=255)                                                          tAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCa                                                                          <  1:927836/138‑1 (MQ=255)                                                          tAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCa                                                                          >  2:927836/1‑138 (MQ=255)                                                          tAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAgc                                                                        >  2:36625/1‑140 (MQ=255)                                                           aaaCGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAgcg                                                                       <  1:597324/140‑1 (MQ=255)                                                           aaaCGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAgcg                                                                       <  2:235062/140‑1 (MQ=255)                                                           aaaCGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTACGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGACGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAgcg                                                                       <  2:1014429/140‑1 (MQ=255)                                                            aacgaacgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGa                                                                                      >  2:430451/1‑124 (MQ=255)                                                            aacgaacgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGa                                                                                      <  1:430451/124‑1 (MQ=255)                                                            aacgaacgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAgcgc                                                                      >  1:450427/1‑140 (MQ=255)                                                               gaacgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCAtt                                                                   <  1:496746/140‑1 (MQ=255)                                                                 acgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGt                                                                 >  1:600641/1‑140 (MQ=255)                                                                    cTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACg                                                              >  1:337851/1‑140 (MQ=255)                                                                       cACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTcg                                                           <  1:373889/140‑2 (MQ=255)                                                                       cACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCa                                                           <  2:386736/140‑1 (MQ=255)                                                                               aTTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCaagta                                                   >  1:529015/1‑140 (MQ=255)                                                                                ttACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCaagtaa                                                  <  2:529015/140‑1 (MQ=255)                                                                                 tACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCaagtaag                                                 >  2:594473/1‑140 (MQ=255)                                                                                 tACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCaagtaag                                                 >  2:989816/1‑140 (MQ=255)                                                                                      gATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTAcg                                            <  1:284007/140‑1 (MQ=255)                                                                                      gATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTAcg                                            >  1:735541/1‑140 (MQ=255)                                                                                        tGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTAcgcg                                          >  2:830394/1‑140 (MQ=255)                                                                                           ccAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCCGCGCATTGTACGTCCGGCAAGTAAGTTACGCgttg                                       >  1:235610/1‑140 (MQ=255)                                                                                              gATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGttt                                    >  2:78207/1‑140 (MQ=255)                                                                                               aTCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGtttt                                   <  2:825475/140‑1 (MQ=255)                                                                                                    tGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGa                              <  2:337851/140‑1 (MQ=255)                                                                                                    tGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGa                              <  2:735541/140‑1 (MQ=255)                                                                                                    tGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGa                              <  2:450427/140‑1 (MQ=255)                                                                                                    tGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGCGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGa                              >  2:117656/1‑140 (MQ=255)                                                                                                         aaGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGAt                         >  1:448694/1‑140 (MQ=255)                                                                                                         aaGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGAt                         >  2:1037629/1‑140 (MQ=255)                                                                                                         aaGTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGAt                         <  2:842550/140‑1 (MQ=255)                                                                                                           gTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGa                       >  2:1078695/1‑140 (MQ=255)                                                                                                           gTTTATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGa                       <  2:290736/140‑1 (MQ=255)                                                                                                              tATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAaga                    <  2:398524/140‑1 (MQ=255)                                                                                                              tATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAaga                    >  1:658859/1‑140 (MQ=255)                                                                                                              tATCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAaga                    >  1:285046/1‑140 (MQ=255)                                                                                                               aTCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAagag                   >  2:579676/1‑140 (MQ=255)                                                                                                                tCTGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAgaga                  >  2:311447/1‑140 (MQ=255)                                                                                                                  tGGCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGAtt                <  1:650270/140‑1 (MQ=255)                                                                                                                   ggCGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTg               <  1:51712/140‑1 (MQ=255)                                                                                                                     cGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAATAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCa             >  2:819986/1‑140 (MQ=255)                                                                                                                     cGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCa             >  2:7162/1‑140 (MQ=255)                                                                                                                     cGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCa             >  2:454763/1‑140 (MQ=255)                                                                                                                     cGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCa             >  2:59155/1‑140 (MQ=255)                                                                                                                     cGATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCa             >  2:41660/1‑140 (MQ=255)                                                                                                                      gATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAg            <  2:600641/140‑1 (MQ=255)                                                                                                                      gATGACCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAg            <  1:311447/140‑1 (MQ=255)                                                                                                                          aCCGGTGGTCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCa        >  1:1041396/1‑140 (MQ=255)                                                                                                                              gtggtCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGc              >  2:1072474/1‑130 (MQ=255)                                                                                                                              gtggtCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGc              <  1:1072474/130‑1 (MQ=255)                                                                                                                              gtggtCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCATGaa    >  2:477113/1‑140 (MQ=255)                                                                                                                              gtggtCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCATGaa    >  2:839459/1‑140 (MQ=255)                                                                                                                                ggtCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCATGAAGc  <  1:830394/140‑1 (MQ=255)                                                                                                                                ggtCAAGCGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCATGAAGc  <  1:839459/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      TCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAAGAGATTGCAGCTCATGAAGC  >  NC_000913/236480‑236746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑