Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,719,230	T→C	T125A (ACC→GCC)	anmK ←	anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,719,230	0	T	C	100.0%	205.1 / NA	58	T125A (ACC→GCC)	anmK	anhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/30);  total (28/30)

CCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCA  >  NC_000913/1719097‑1719364                                                                                                                                      |                                                                                                                                       ccAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGgcgcgtgc                                                                                                                                  <  2:512817/140‑1 (MQ=255)   aaTATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCca                                                                                                                                <  2:581452/140‑1 (MQ=255)    atatTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCcac                                                                                                                               <  2:816833/140‑1 (MQ=255)         gAGCACCATTCGTCACTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAAttt                                                                                                                          >  1:831151/1‑140 (MQ=255)              ccATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTg                                                                                                                     >  1:62372/1‑140 (MQ=255)               cATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGt                                                                                                                    <  2:849067/140‑1 (MQ=255)                aTTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGtt                                                                                                                   >  2:407712/1‑140 (MQ=255)                 ttCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTa                                                                                                                  >  2:1047650/1‑140 (MQ=255)                 ttCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTa                                                                                                                  >  2:889192/1‑140 (MQ=255)                      cgCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGcc                                                                                                             <  1:655676/140‑1 (MQ=255)                      cgCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGcc                                                                                                             <  1:1047650/140‑1 (MQ=255)                           ggTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCt                                                                                                        >  2:301050/1‑140 (MQ=255)                                     gCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAgtgtg                                                                                              >  1:684814/1‑140 (MQ=255)                                      ccAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAgtgtgt                                                                                             <  1:407712/140‑1 (MQ=255)                                      ccAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAgtgtgt                                                                                             <  1:640977/140‑1 (MQ=255)                                      ccAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAgtgtgt                                                                                             >  1:140115/1‑140 (MQ=255)                                       cagcagCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGCAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGtgtgtg                                                                                            >  2:163319/1‑140 (MQ=255)                                          cagcGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAgtgtg                                                                                              >  2:994664/1‑135 (MQ=255)                                          cagcGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAgtgtg                                                                                              <  1:994664/135‑1 (MQ=255)                                                    tgGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGt                                                                               <  2:191461/140‑1 (MQ=255)                                                    tgGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGt                                                                               <  1:140959/140‑1 (MQ=255)                                                        aCGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGt                                                                           <  2:799810/140‑1 (MQ=255)                                                        aCGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGt                                                                           <  2:831151/140‑1 (MQ=255)                                                        aCGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGt                                                                           <  1:301050/140‑1 (MQ=255)                                                            aGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCAt                                                                       >  2:534759/1‑140 (MQ=255)                                                            aGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCAt                                                                       >  1:443627/1‑140 (MQ=255)                                                              gTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGc                                                                     >  2:533612/1‑140 (MQ=255)                                                                aCCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCa                                                                   <  2:62372/140‑1 (MQ=255)                                                                        tGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGttt                                                           >  2:1084873/1‑140 (MQ=255)                                                                        tGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGttt                                                           >  1:454348/1‑140 (MQ=255)                                                                         gcgcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTg                                                          <  1:1084873/140‑1 (MQ=255)                                                                         gcgcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTg                                                          >  2:259281/1‑140 (MQ=255)                                                                          cgcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGa                                                                 >  1:94410/1‑132 (MQ=255)                                                                          cgcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGa                                                                 <  2:94410/132‑1 (MQ=255)                                                                          cgcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGa                                                         >  1:470744/1‑140 (MQ=255)                                                                            ccTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGAcc                                                       >  1:270304/1‑140 (MQ=255)                                                                               tGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTg                                                    >  1:402263/1‑140 (MQ=255)                                                                                     cccAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAAcc                                              >  1:826947/1‑140 (MQ=255)                                                                                     cccAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAAcc                                              <  2:443627/140‑1 (MQ=255)                                                                                        aaGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGAt                                           <  1:889192/140‑1 (MQ=255)                                                                                         agacAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATc                                          >  1:955352/4‑140 (MQ=255)                                                                                          ggCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCg                                         <  2:826947/140‑1 (MQ=255)                                                                                           gCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAAc                                               >  2:435493/1‑133 (MQ=255)                                                                                           gCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAAc                                               <  1:435493/133‑1 (MQ=255)                                                                                           gCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCAAAATTTGATTGTTATCGCCAATCTGTAAAGGGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGATCGTGACAACCGATCGc                                        >  1:385908/1‑140 (MQ=255)                                                                                             aaTATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCaa                                      >  1:938643/1‑140 (MQ=255)                                                                                                atCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACta                                   <  2:470744/140‑1 (MQ=255)                                                                                                atCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACta                                   <  1:259281/140‑1 (MQ=255)                                                                                                  cgcgACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGACAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACtata                                 >  2:968879/1‑140 (MQ=255)                                                                                                    cgACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCg                                         <  2:800852/130‑1 (MQ=255)                                                                                                    cgACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCg                                         >  1:800852/1‑130 (MQ=255)                                                                                                     gACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGAt                                           <  2:860067/127‑1 (MQ=255)                                                                                                     gACGGCGAAAATCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGAt                                           >  1:860067/1‑127 (MQ=255)                                                                                                              aaTCACCGACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGc                     <  2:402263/140‑1 (MQ=255)                                                                                                                     gACAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGAtttt              <  2:270304/140‑1 (MQ=255)                                                                                                                      aCAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTg             <  2:859953/140‑1 (MQ=255)                                                                                                                      aCAACCGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTg             <  2:912211/140‑1 (MQ=255)                                                                                                                           cGTAATTCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCt        >  2:891773/1‑140 (MQ=255)                                                                                                                                ttCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGc   <  1:534759/140‑1 (MQ=255)                                                                                                                                 tCCGGCGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCa  <  1:64130/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       CCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCA  >  NC_000913/1719097‑1719364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑