Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,758,523	G→A	A68V (GCC→GTC)	ldtE ←	L,D‑transpeptidase LdtE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,758,523	0	G	A	100.0%	110.7 / NA	32	A68V (GCC→GTC)	ldtE	L,D‑transpeptidase LdtE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/15);  total (17/15)

CGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGT  >  NC_000913/1758390‑1758642                                                                                                                                      |                                                                                                                        cggcggATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCaa                                                                                                                   >  2:240612/1‑140 (MQ=255)                 aGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGc                                                                                                   <  2:588892/140‑1 (MQ=255)                   gcgcAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAg                                                                                                 <  1:747380/140‑1 (MQ=255)                             gCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGTTAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACg                                                                                       <  2:945887/140‑1 (MQ=255)                                   ttAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGc                                                                                 >  1:70927/1‑140 (MQ=255)                                       cGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATa                                                                             >  2:384637/1‑140 (MQ=255)                                             tCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTc                                                                                     >  1:1041424/1‑126 (MQ=255)                                             tCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTc                                                                                     <  2:1041424/126‑1 (MQ=255)                                             tCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCaa                                                                               <  1:418359/132‑1 (MQ=255)                                             tCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCaa                                                                               >  2:418359/1‑132 (MQ=255)                                                ccTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAgg                                                                    <  1:384637/140‑1 (MQ=255)                                                       cgGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTAt                                                             <  1:148596/140‑1 (MQ=255)                                                         gTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATcc                                                           >  1:625077/1‑140 (MQ=255)                                                                   taataaCAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCa                                                 <  1:591812/140‑1 (MQ=255)                                                                        aCAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGta                                            <  2:70927/140‑1 (MQ=255)                                                                            ttGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCa                                                 <  1:1043076/131‑1 (MQ=255)                                                                            ttGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCa                                                 >  2:1043076/1‑131 (MQ=255)                                                                               tgAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGttt                                     >  2:736353/1‑140 (MQ=255)                                                                                  aGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGAt                                  <  1:1039868/140‑1 (MQ=255)                                                                                           tATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCaa                         >  2:285004/1‑140 (MQ=255)                                                                                            aTCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAAc                        <  1:736353/140‑1 (MQ=255)                                                                                            aTCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAAc                        >  2:858203/1‑140 (MQ=255)                                                                                              cgtcgtACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCa                      >  2:897578/1‑140 (MQ=255)                                                                                               gtcgtACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAg                     >  1:870756/1‑140 (MQ=255)                                                                                               gtcgtACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAg                     >  1:1021118/1‑140 (MQ=255)                                                                                                 cgtACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTc                   >  1:867492/1‑140 (MQ=255)                                                                                                       aGGTTTTGGCACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTc             <  2:867492/140‑1 (MQ=255)                                                                                                          ttttGGCACCGGCGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCgg          >  1:908920/1‑140 (MQ=255)                                                                                                                cACCGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTtggtg    <  2:870756/140‑1 (MQ=255)                                                                                                                  ccGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTggtggt  >  1:465061/1‑140 (MQ=255)                                                                                                                   cGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTc             >  2:600981/1‑128 (MQ=255)                                                                                                                   cGGGGCGATAGTGTTATTGACTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTc             <  1:600981/128‑1 (MQ=255)                                                                                                                                      |                                                                                                                        CGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGT  >  NC_000913/1758390‑1758642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑