Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,886,797	T→C	V318A (GTG→GCG)	yeaX →	carnitine monooxygenase subunit YeaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,886,797	0	T	C	100.0%	67.1 / NA	20	V318A (GTG→GCG)	yeaX	carnitine monooxygenase subunit YeaX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/9);  total (11/9)

TGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCG  >  NC_000913/1886667‑1886927                                                                                                                                   |                                                                                                                                   tGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGAttt                                                                                                                           >  1:419798/1‑140 (MQ=255)      aaCCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGgcgt                                                                                                                                  >  1:36325/1‑128 (MQ=255)           gCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGcc                                                                                                                 >  2:342065/1‑140 (MQ=255)                 cAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGcc                                                                                                           <  1:467758/140‑1 (MQ=255)                                 tGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGcc                                                                                                           >  1:125619/1‑124 (MQ=255)                                 tGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGcc                                                                                                           <  2:125619/124‑1 (MQ=255)                                 tGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAgc                                                                                           >  2:470367/1‑140 (MQ=255)                                          ccATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGt                                                                                  <  1:826505/140‑1 (MQ=255)                                           cATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGTCGGATGTGGCGCTGAGCGCGCCCAGTc                                                                                 <  2:419798/140‑1 (MQ=255)                                                   taaaTATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCg                                                                         >  1:1043918/3‑140 (MQ=255)                                                            tAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGAtttt                                                                <  1:28863/140‑1 (MQ=255)                                                                    aaGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGa                                                        <  1:860622/140‑1 (MQ=255)                                                                                          tATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTa                                  >  2:947226/1‑140 (MQ=255)                                                                                             gTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATg                               <  1:342065/140‑1 (MQ=255)                                                                                                    tgttgtTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGc                        >  1:666991/1‑140 (MQ=255)                                                                                                    tgttgtTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGc                        >  2:881381/1‑140 (MQ=255)                                                                                                    tgttgtTCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGc                        >  1:182047/1‑140 (MQ=255)                                                                                                        gttCGCGTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAATCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATc                    >  1:529084/1‑140 (MQ=255)                                                                                                              gTGCGAAGGGTAAACGCCTGGCGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTca              >  2:948717/1‑140 (MQ=255)                                                                                                                          aaCGCCTGGCGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCg  <  1:470367/140‑1 (MQ=255)                                                                                                                          aaCGCCTGGCGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAATCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCg  <  2:529084/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                   TGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCG  >  NC_000913/1886667‑1886927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑