Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	254,329	A→G	V463A (GTT→GCT)	pepD ←	peptidase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	254,329	0	A	G	100.0%	145.2 / NA	41	V463A (GTT→GCT)	pepD	peptidase D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/23);  total (18/23)

GAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAA  >  NC_000913/254194‑254462                                                                                                                                        |                                                                                                                                      gAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTg                                                                                                                                   <  1:593233/140‑1 (MQ=255) gAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTg                                                                                                                                   <  1:648771/140‑1 (MQ=255)  aaGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGc                                                                                                                                  >  2:83074/1‑140 (MQ=255)  aaGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGc                                                                                                                                  >  1:681045/1‑140 (MQ=255)   aGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCt                                                                                                                                 >  1:904590/1‑140 (MQ=255)   aGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCt                                                                                                                                 <  1:161333/140‑1 (MQ=255)   aGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCt                                                                                                                                 <  1:465925/140‑1 (MQ=255)   aGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCt                                                                                                                                 <  2:302491/140‑1 (MQ=255)          tGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCgg                                                                                                                          <  2:22882/140‑1 (MQ=255)          tGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCgg                                                                                                                          <  2:713507/140‑1 (MQ=255)          tGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCgg                                                                                                                          <  2:708697/140‑1 (MQ=255)           gAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGga                                                                                                                         <  2:901555/140‑1 (MQ=255)               gATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGagagg                                                                                                                     >  1:776394/1‑139 (MQ=255)               gATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAgt                                                                                                                     >  2:631024/1‑140 (MQ=255)                         ccGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGACAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGt                                                                                                           >  2:944732/1‑140 (MQ=255)                                   aTTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGc                                                                                                 <  2:1017552/140‑1 (MQ=255)                                    ttAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGcc                                                                                                <  2:294326/140‑1 (MQ=255)                                         cGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATa                                                                                           <  2:735596/140‑1 (MQ=255)                                              tCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAAc                                                                                      >  1:311883/1‑140 (MQ=255)                                               ccGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAAcc                                                                                     <  1:299520/140‑1 (MQ=255)                                                 ggCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGt                                                                                                     >  2:989449/1‑122 (MQ=255)                                                 ggCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGt                                                                                                     <  1:989449/122‑1 (MQ=255)                                                               taatTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGAt                                                                     <  2:311883/140‑1 (MQ=255)                                                                aatTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGGGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATa                                                                    >  1:703686/1‑140 (MQ=255)                                                                  ttACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACg                                                                  <  1:638342/140‑1 (MQ=255)                                                                            ggAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGaa                                                        <  1:597226/140‑1 (MQ=255)                                                                            ggAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGaa                                                        <  2:367322/140‑1 (MQ=255)                                                                                            gTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAgg                                        <  1:944732/140‑1 (MQ=255)                                                                                                       agTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGa                              >  2:425192/1‑139 (MQ=255)                                                                                                       agTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGa                              <  1:425192/139‑1 (MQ=255)                                                                                                       agTGTCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGAt                             >  1:5475/1‑140 (MQ=255)                                                                                                           tCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGAt                             <  2:339047/136‑1 (MQ=255)                                                                                                           tCCAGTAATGACCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGAt                             >  1:339047/1‑136 (MQ=255)                                                                                                                     aCCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCg               >  2:367052/1‑140 (MQ=255)                                                                                                                     aCCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCg               >  2:52207/1‑140 (MQ=255)                                                                                                                     aCCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCg               >  1:734312/1‑140 (MQ=255)                                                                                                                     aCCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCg               >  1:560613/1‑140 (MQ=255)                                                                                                                     aCCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCg               >  1:442478/1‑140 (MQ=255)                                                                                                                     aCCTACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCg               >  1:437967/1‑140 (MQ=255)                                                                                                                        tACGCTTTCGATGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCg            <  2:904590/140‑1 (MQ=255)                                                                                                                                  aTGTGAGCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGaa  <  2:681045/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      GAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAA  >  NC_000913/254194‑254462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑