| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,910,848 | G→A | L191L (CTG→CTA) | yebQ → | putative transporter YebQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,910,848 | 0 | G | A | 97.7% | 134.7 / ‑4.1 | 43 | L191L (CTG→CTA) | yebQ | putative transporter YebQ |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (20/22); total (21/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01 | |||||||||||
GCCGTCTCTTCTGCTGCCGGGCCGACAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTGACGTTAATTGCTGCG > NC_000913/1910723‑1910983 | gCCGTCTCTTCTGCTGCCGGGCCGACAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTtc > 1:692303/1‑140 (MQ=255) ccGTCTCTTCTGCTGCCGGGCCGACAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTtct < 2:173516/140‑1 (MQ=255) ctctTCTGCTGCCGGGCCGACAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTcgcg > 2:801638/1‑140 (MQ=255) ctctTCTGCTGCCGGGCCGACAATTGCTGCAGCAATCCTCCCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTcgcg < 2:374034/140‑1 (MQ=255) tcttctGCTGCCGGGCCGACAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCgcgc < 2:446046/140‑1 (MQ=255) cAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGAc < 2:603139/140‑1 (MQ=255) cAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGAc < 2:1061012/140‑1 (MQ=255) cAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGAc < 2:568412/140‑1 (MQ=255) aaTTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGAcc > 1:780401/1‑140 (MQ=255) agcaATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAgcg < 2:229307/140‑1 (MQ=255) gcaATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAgcgc > 2:349308/1‑140 (MQ=255) aTCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGt > 2:600216/1‑140 (MQ=255) ctcCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGtgatga < 1:801638/140‑1 (MQ=255) ctcCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGtgatga < 1:84921/140‑1 (MQ=255) cGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGt > 1:744714/1‑140 (MQ=255) cATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTa > 1:960389/1‑140 (MQ=255) tGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTGAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCtt < 2:543057/140‑1 (MQ=255) tGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTGAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCtt > 1:543057/1‑140 (MQ=255) tGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCtt < 2:718793/140‑1 (MQ=255) aaTGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGc < 2:627306/140‑1 (MQ=255) gTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGAGATGAACGCGTTAACCTTCGGCCTGc > 2:962013/1‑140 (MQ=255) tttttAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTAt > 1:729847/1‑140 (MQ=255) aTCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCt > 2:39759/1‑128 (MQ=255) aTCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCt < 1:39759/128‑1 (MQ=255) aTCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGc > 1:216892/1‑140 (MQ=255) cGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGa > 1:1066050/1‑140 (MQ=255) cGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGa < 1:379406/140‑1 (MQ=255) gTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAg < 2:744714/140‑1 (MQ=255) ggTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACt < 2:447528/123‑1 (MQ=255) ggTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACt > 1:447528/1‑123 (MQ=255) gTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCt < 2:1066050/140‑1 (MQ=255) tatCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGc > 1:1056198/1‑135 (MQ=255) tatCGCCCTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGc < 2:1056198/135‑1 (MQ=255) cccTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCaa > 2:816473/1‑140 (MQ=255) cTGCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATc > 2:61075/1‑140 (MQ=255) gCTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGc < 2:109655/140‑1 (MQ=255) cTTCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCt < 1:61075/140‑1 (MQ=255) ttCTGGCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTg < 2:960389/140‑1 (MQ=255) gCGATGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTGACGtt > 2:17390/1‑140 (MQ=255) tGCGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTGACGTTAAtt > 2:671325/1‑140 (MQ=255) cGTTTTCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTGACGTTAATtgc > 1:975635/1‑140 (MQ=255) ttttCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTGACGTTAATtgctg > 2:317981/1‑140 (MQ=255) ttCTACCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTGACGTTAATTGCTGCg < 1:73308/140‑1 (MQ=255) | GCCGTCTCTTCTGCTGCCGGGCCGACAATTGCTGCAGCAATCCTCTCCATCGCATCCTGGAAATGGTTATTTTTAATCAACGTACCGTTAGGTATTATCGCCCTGCTTCTGGCGATGCGTTTTCTGCCACCCAATGGTTCTCGCGCCAGTAAACCCCGTTTCGACCTGCCCAGCGCCGTGATGAACGCGTTAACCTTCGGCCTGCTTATCACTGCGTTGAGTGGTTTCGCTCAGGGGCAATCGCTGACGTTAATTGCTGCG > NC_000913/1910723‑1910983 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |