Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,130,735	T→C	E48E (GAA→GAG)	wcaD ←	putative colanic acid polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,130,735	0	T	C	100.0%	211.1 / NA	58	E48E (GAA→GAG)	wcaD	putative colanic acid polymerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/34);  total (24/34)

GATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAG  >  NC_000913/2130600‑2130871                                                                                                                                        |                                                                                                                                         gATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTa                                                                                                                                      >  2:338182/1‑140 (MQ=255)  aTCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTaa                                                                                                                                     >  2:926986/1‑140 (MQ=255)   tCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTaaa                                                                                                                                    <  1:189465/140‑1 (MQ=255)        atatAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAAcag                                                                                                                               <  1:338182/140‑1 (MQ=255)         tataGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAAcagc                                                                                                                              <  1:167375/140‑1 (MQ=255)         tataGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAAcagc                                                                                                                              <  1:859034/140‑1 (MQ=255)         tataGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAAcagc                                                                                                                              <  1:739266/140‑1 (MQ=255)         tataGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCACCAATTTTTTAACGCTGATGCGCTCTAAAAAcagc                                                                                                                              <  1:1062226/140‑1 (MQ=255)              aCAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTaa                                                                                                                         >  2:481483/1‑140 (MQ=255)                 aaCAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACaa                                                                                                                      >  1:931001/1‑140 (MQ=255)                  aCAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGCGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAg                                                                                                                     >  1:178172/1‑140 (MQ=255)                    aGCATAGTTGAAGTGTCGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGAc                                                                                                                   <  1:980224/140‑1 (MQ=255)                    aGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGAc                                                                                                                   <  1:919465/140‑1 (MQ=255)                    aGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGAc                                                                                                                   <  1:926986/140‑1 (MQ=255)                     gCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAATCAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACa                                                                                                                  >  2:8330/1‑140 (MQ=255)                              aaGTGACGTATTTACTGGCATCCAGCGACTGACCAATCAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTaaaaaa                                                                                                         <  1:8330/140‑1 (MQ=255)                              aaGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGTGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTaaaaaa                                                                                                         <  1:376914/140‑1 (MQ=255)                                gTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGt                                                                                                       <  1:131635/140‑1 (MQ=255)                                     gTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGa                                                                                                  >  2:683058/1‑140 (MQ=255)                                     gTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGa                                                                                                  <  2:443597/140‑1 (MQ=255)                                       aTTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATg                                                                                                <  1:788189/140‑1 (MQ=255)                                         ttACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGt                                                                                              <  1:683058/140‑1 (MQ=255)                                               gCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATggg                                                                                        >  2:480371/1‑140 (MQ=255)                                                cATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATgggg                                                                                       <  1:480371/140‑1 (MQ=255)                                                  tCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTg                                                                                             <  1:99461/132‑1 (MQ=255)                                                  tCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTg                                                                                             >  2:99461/1‑132 (MQ=255)                                                           tGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGcc                                                                            >  2:143731/1‑140 (MQ=255)                                                           tGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGcc                                                                            >  1:775595/1‑140 (MQ=255)                                                             aCCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCGATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCaa                                                                          >  1:88190/1‑140 (MQ=255)                                                                       tAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGAt                                                                >  2:330204/1‑140 (MQ=255)                                                                       tAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGAt                                                                >  2:210092/1‑140 (MQ=255)                                                                         gTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGAttt                                                              <  1:210092/140‑1 (MQ=255)                                                                         gTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGAttt                                                              <  2:180350/140‑1 (MQ=255)                                                                            aaaCGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGa                                                           <  1:913408/140‑1 (MQ=255)                                                                            aaaCGCCGCGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGa                                                           <  2:178172/140‑1 (MQ=255)                                                                                gCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGtt                                                       >  2:912264/1‑140 (MQ=255)                                                                                   gTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAAc                                                    <  1:912264/140‑1 (MQ=255)                                                                                    tGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAAcc                                                   >  1:1029181/1‑140 (MQ=255)                                                                                    tGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGCGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAAcc                                                   >  2:707794/1‑140 (MQ=255)                                                                                     gAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACca                                                  <  2:775595/140‑1 (MQ=255)                                                                                                  aTCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAg                                     >  2:13878/1‑140 (MQ=255)                                                                                                        aaGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGgc                                 >  1:941237/1‑138 (MQ=255)                                                                                                        aaGGCAATCATCAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGgc                                 <  2:941237/138‑1 (MQ=255)                                                                                                              atcatcAATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAg                         >  2:355775/1‑140 (MQ=255)                                                                                                               tcatcaATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGgcag                               <  2:928651/133‑1 (MQ=255)                                                                                                               tcatcaATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGgcag                               >  1:928651/1‑133 (MQ=255)                                                                                                               tcatcaATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGgc                                 <  1:907401/131‑1 (MQ=255)                                                                                                               tcatcaATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGgc                                 >  2:907401/1‑131 (MQ=255)                                                                                                               tcatcaATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAgg                        >  1:10629/1‑140 (MQ=255)                                                                                                               tcatcaATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAgg                        >  2:296636/1‑140 (MQ=255)                                                                                                               tcatcaATTTTTTAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAgg                        <  1:143731/140‑1 (MQ=255)                                                                                                                          ttAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGAt             <  1:961250/140‑1 (MQ=255)                                                                                                                           tAACGCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGAtt            <  1:635422/140‑1 (MQ=255)                                                                                                                               gCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGa        <  1:13878/140‑1 (MQ=255)                                                                                                                               gCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGa        <  1:330204/140‑1 (MQ=255)                                                                                                                               gCTGATGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGa        <  1:1009368/140‑1 (MQ=255)                                                                                                                                   aTGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGa    <  1:296636/140‑1 (MQ=255)                                                                                                                                   aTGCGCTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGa    <  1:906838/140‑1 (MQ=255)                                                                                                                                     gcgcTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAg  <  2:1029181/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAG  >  NC_000913/2130600‑2130871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑