Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,338,438	A→G	V328A (GTT→GCT)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,338,438	0	A	G	100.0%	104.5 / NA	32	V328A (GTT→GCT)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/15);  total (17/15)

TACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTT  >  NC_000913/2338307‑2338572                                                                                                                                    |                                                                                                                                       tACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAAc                                                                                                                                >  1:140140/1‑140 (MQ=255)        ttCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCt                                                                                                                         >  2:860921/1‑140 (MQ=255)              aaTAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGt                                                                                                                   <  1:860921/140‑1 (MQ=255)                tAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTag                                                                                                                 <  1:293804/140‑1 (MQ=255)                  gTACGACGGGTCACCACTTCACGGCGGTGGCGAACAAACGCCGCGGTGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGGTACCGAAAGAAGCCTGCAACTGGGTCTGGGGGTagag                                                                                                               >  2:992095/1‑140 (MQ=255)                   tACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAgg                                                                                                              >  1:212759/1‑140 (MQ=255)                   gacgacgGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAgg                                                                                                              <  1:406605/139‑1 (MQ=255)                        cgGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGgttgtt                                                                                                         <  2:212759/140‑1 (MQ=255)                           gTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAg                                                                                                      >  2:1026804/1‑140 (MQ=255)                           gTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAg                                                                                                      >  2:232879/1‑140 (MQ=255)                           gTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAg                                                                                                      <  1:87697/140‑1 (MQ=255)                           gTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAg                                                                                                      >  1:685211/1‑140 (MQ=255)                                cacTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACaa                                                                                                 >  1:211043/1‑140 (MQ=255)                                     cACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCa                                                                                            >  2:1006545/1‑140 (MQ=255)                                             tGACGGACAAACCCCCCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCAAGGGGCAATGCCACCATATTGATACCGAAAGAAGCCTGCAACTGGGGCTGGGAGTAGAGGTTGTTGAGCACAACATTACCCACCGc                                                                                    >  2:755900/1‑140 (MQ=255)                                              gACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCa                                                                                   <  2:685211/140‑1 (MQ=255)                                                       aCGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTc                                                                          >  1:1046214/1‑140 (MQ=255)                                                                   tgTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACg                                                              <  1:670767/140‑1 (MQ=255)                                                                   tgTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACg                                                              <  2:366223/140‑1 (MQ=255)                                                                            ggTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATa                                                     <  2:1046214/140‑1 (MQ=255)                                                                              ttCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATAcc                                                   <  1:1026804/140‑1 (MQ=255)                                                                                        tCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCa                                         >  2:437923/1‑140 (MQ=255)                                                                                         cGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAg                                        <  1:437923/140‑1 (MQ=255)                                                                                         cGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAg                                        >  1:298088/1‑140 (MQ=255)                                                                                         cGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAg                                        >  2:326435/1‑140 (MQ=255)                                                                                         cGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAg                                        <  2:397170/140‑1 (MQ=255)                                                                                               aCCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTg                                            >  1:475458/1‑130 (MQ=255)                                                                                               aCCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTg                                            <  2:475458/130‑1 (MQ=255)                                                                                               aCCATGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGt                                  >  1:1030827/1‑140 (MQ=255)                                                                                                   tGGTGCAATGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACg                              <  2:1027408/140‑1 (MQ=255)                                                                                                          aTGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGAc                                      <  2:1085604/125‑1 (MQ=255)                                                                                                          aTGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGAc                                      >  1:1085604/1‑125 (MQ=255)                                                                                                           tGCCACCATGTTGATACCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAgcgcgc                      >  2:581389/1‑140 (MQ=255)                                                                                                                          aCCGAAAGAAGCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCAcgc       >  2:967503/1‑140 (MQ=255)                                                                                                                             gaaagaagCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGtt    <  1:326435/140‑1 (MQ=255)                                                                                                                              aaagaagCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGttt   >  2:581187/1‑140 (MQ=255)                                                                                                                               aagaagCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGa                   >  2:46466/1‑123 (MQ=255)                                                                                                                               aagaagCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGa                   <  1:46466/123‑1 (MQ=255)                                                                                                                               aagaagCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGtttt  >  2:597333/1‑140 (MQ=255)                                                                                                                               aagaagCCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGtttt  >  2:824205/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                       TACGCAGTTCGAAAATAGTACGACGGGTCACCACTTCACGGCGGTGACGAACAAACGCCGCGATGATGTCTTTCAGGTTCATGATCTTCGGCTGACCATGGTGCAATGCCACCATGTTGATACCGAAAGAAACCTGCAACTGGGTCTGGGAGTAGAGGTTGTTGAGCACAACTTCACCGACCGCATCGCGTTTCACTTCAATCACGATGCGCATACCGTCTTTGTCAGACTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTT  >  NC_000913/2338307‑2338572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑