Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,417,868	T→C	V263A (GTG→GCG)	yfcC →	putative transporter YfcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,417,868	0	T	C	100.0%	178.6 / NA	51	V263A (GTG→GCG)	yfcC	putative transporter YfcC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/21);  total (30/21)

CAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGTCTGGGTGATTTGGGGCGTGATCGTTAATGCC  >  NC_000913/2417732‑2418004                                                                                                                                         |                                                                                                                                         cAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCa                                                                                                                                       <  1:298931/140‑1 (MQ=255)       aTTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTc                                                                                                                                 >  1:954656/1‑140 (MQ=255)         tGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCg                                                                                                                               >  2:151139/1‑140 (MQ=255)              gCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGc                                                                                                                          >  1:715400/1‑140 (MQ=255)                gTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGctt                                                                                                                        <  1:43344/140‑1 (MQ=255)                   ccGGTGCTTTCTGGCTCCGGGTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGcttctt                                                                                                                     >  2:363586/1‑140 (MQ=255)                         cTTTCTGGCTCCGGGTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGa                                                                                                               <  1:363586/140‑1 (MQ=255)                             cTGGCTCCGGGTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAg                                                                                                           <  2:237761/140‑1 (MQ=255)                               ggCTCCGGGTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCa                                                                                                         <  2:998052/140‑1 (MQ=255)                                gCTCCGGGTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAg                                                                                                        >  2:564102/1‑140 (MQ=255)                                  tCCGGGTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGc                                                                                                      <  1:151139/140‑1 (MQ=255)                                  tCCGGGTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGc                                                                                                      <  1:564102/140‑1 (MQ=255)                                      ggTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGAt                                                                                                  >  1:467696/1‑140 (MQ=255)                                           cgcATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGc                                                                                                      <  1:259928/131‑1 (MQ=255)                                           cgcATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGc                                                                                                      >  2:259928/1‑131 (MQ=255)                                           cgcATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGa                                                                                             >  1:903626/1‑140 (MQ=255)                                             cATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGaac                                                                                           <  1:165093/140‑1 (MQ=255)                                               tCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGaacaa                                                                                         <  2:1000588/140‑1 (MQ=255)                                                  tggtgTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGt                                                                                      >  2:640250/1‑140 (MQ=255)                                                         ggTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTa                                                                               <  1:891541/140‑1 (MQ=255)                                                          gTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTAc                                                                              >  1:377002/1‑140 (MQ=255)                                                          gTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTAc                                                                              >  2:991496/1‑140 (MQ=255)                                                            tATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCt                                                                            >  1:465075/1‑140 (MQ=255)                                                               cGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACg                                                                                       <  2:923593/126‑1 (MQ=255)                                                               cGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACg                                                                                       >  1:923593/1‑126 (MQ=255)                                                                   aCTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGa                                                                     <  2:715400/140‑1 (MQ=255)                                                                   aCTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGa                                                                     <  2:467696/140‑1 (MQ=255)                                                                    ctctGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGAc                                                                    >  1:756956/1‑140 (MQ=255)                                                                     tctGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGAct                                                                   <  2:903626/140‑1 (MQ=255)                                                                       tGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGActgg                                                                 <  2:465075/140‑1 (MQ=255)                                                                       tGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGActgg                                                                 <  1:1041082/140‑1 (MQ=255)                                                                              ccTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGa                                                                     >  2:187377/1‑129 (MQ=255)                                                                              ccTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGa                                                                     <  1:187377/129‑1 (MQ=255)                                                                                   tCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGgattgatt                                                     >  2:835487/1‑140 (MQ=255)                                                                                       tACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTcc                                                 >  1:742179/1‑140 (MQ=255)                                                                                           caggTGTACCCCTCACGAGTAAAAAAAAACCCTCTTCTGTCACGCGCGCATGAGCCCGCCCGTTTTTTTCGTGAAAAGCGGGCTGATGTTGAACAACGTCCTTTTCCTTTTGGTGACTGGCTGGTATTGATTGTCCTGAc                                             <  2:814021/138‑1 (MQ=255)                                                                                           aTGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGAc                                             <  1:548508/140‑1 (MQ=255)                                                                                            tGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGt                                                            >  1:25838/1‑124 (MQ=255)                                                                                            tGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGt                                                            <  2:25838/124‑1 (MQ=255)                                                                                            tGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGAcc                                            >  1:146319/1‑140 (MQ=255)                                                                                              gtgtACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGAccgc                                          >  1:536978/1‑140 (MQ=255)                                                                                               tgtACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGAccgcc                                         >  1:516196/1‑140 (MQ=255)                                                                                                 tACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGAccg                                           <  1:958909/136‑1 (MQ=255)                                                                                                 tACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGAccg                                           >  2:958909/1‑136 (MQ=255)                                                                                                  ccGCCCCACGAGTGAAAAAGAATCCTCTTCTGTCCCGCGCGCATGAGCCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCTTTTCCTTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTa                                      <  2:516196/139‑1 (MQ=255)                                                                                                   cGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTaa                                     >  2:507541/1‑140 (MQ=255)                                                                                                       tCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGt                                 >  2:894891/1‑140 (MQ=255)                                                                                                        cACGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGTc                                >  1:756621/1‑140 (MQ=255)                                                                                                         aCGAGTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATggctg                               >  1:814021/1‑137 (MQ=255)                                                                                                             gTGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGCAATGGTCTGGGt                           <  2:954656/140‑1 (MQ=255)                                                                                                              tGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGTCTGGGTg                          >  1:118882/1‑140 (MQ=255)                                                                                                              tGAAAAAGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGTCTGGGTg                          >  2:236887/1‑140 (MQ=255)                                                                                                                aaaaaGAATCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGTCTGGGTGAt                        >  2:55625/1‑140 (MQ=255)                                                                                                                        tCCTCTTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGTCTGGGTGATTTGGGGCg                >  1:314408/1‑140 (MQ=255)                                                                                                                          ctctTCTGTCACGCGCGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGTCTGGGTGATTTGGGGCGTg              <  1:236887/140‑1 (MQ=255)                                                                                                                           tcttctGCCGCGCGCGCGGGGGCCCGCCCCTTTTTTTCGTAAAAGGAGGGCTGATGTTAAACAACGTCCGTTTCCTTTTGGGGATGGGTTGGTTTTGATTGTCCTGACCGCCGTAATGGTCTGGGTGATTTGGGGCGTGa             <  2:536978/140‑1 (MQ=255)                                                                                                                                cgTCACGCGAGCATGATTCCGCCCTCTTCTTTTGGGAAAAGCGGGCTTATTTTGAACAACGTCCGTTTTCCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGTCTGGGTGATTTGGGGCGTGATCGtt        <  2:756621/139‑1 (MQ=255)                                                                                                                                      gcgcgcATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGTCTGGGTGATTTGGGGCGTGATCGTTAATGcc  <  1:991496/140‑1 (MQ=255)                                                                                                                                      gcgcgcATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGTCTGGGTGATTTGGGGCGTGATCGTTAATGcc  <  1:894891/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATCGTGGTGTGGGTTATCGCCACTCTGATTGGCCTGATCTTTACCATGGTGTACGCCTCACGAGTGAAAAAGAATCCTCTTCTGTCACGCGTGCATGAGTCCGACCGCTTCTTTCGTGAAAAGCAGGCTGATGTTGAACAACGTCCGTTTACCTTTGGTGACTGGCTGGTATTGATTGTCCTGACCGCCGTAATGGTCTGGGTGATTTGGGGCGTGATCGTTAATGCC  >  NC_000913/2417732‑2418004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑