Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,471,844	T→C	pseudogene (219/519 nt)	yfdL ←	CPS‑53 (KpLE1) prophage; putative uncharacterized protein YfdL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,471,844	0	T	C	100.0%	141.6 / NA	40	pseudogene (219/519 nt)	yfdL	CPS‑53 (KpLE1) prophage; putative uncharacterized protein YfdL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/18);  total (22/18)

AAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCG  >  NC_000913/2471719‑2471980                                                                                                                              |                                                                                                                                         aaaTAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGcccc                                                                                                                            <  2:208166/140‑1 (MQ=255)            tgtgTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACAt                                                                                                                 >  2:239099/1‑140 (MQ=255)              tgtCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACAttt                                                                                                               >  2:802520/1‑140 (MQ=255)                 ccTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCtt                                                                                                            >  2:169766/1‑140 (MQ=255)                     agaCGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAg                                                                                                        >  2:795173/1‑140 (MQ=255)                     agaCGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAg                                                                                                        >  2:239116/1‑140 (MQ=255)                        cGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATGTCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCg                                                                                                     >  2:489816/1‑140 (MQ=255)                        cGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCg                                                                                                     >  1:646375/1‑140 (MQ=255)                          tCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCt                                                                                                   >  1:230874/1‑140 (MQ=255)                                        aTTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAg                                                                                     >  1:267221/1‑140 (MQ=255)                                          tGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGt                                                                                   <  2:646375/140‑1 (MQ=255)                                           ggCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGtt                                                                                  <  2:230874/140‑1 (MQ=255)                                           ggCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGtt                                                                                  >  2:886504/1‑140 (MQ=255)                                           ggCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGtt                                                                                  >  2:874440/1‑140 (MQ=255)                                                 cATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCgag                                                                            >  2:702253/1‑140 (MQ=255)                                                  aTTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCgaga                                                                           <  2:908505/140‑1 (MQ=255)                                                  aTTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCgaga                                                                           <  1:239116/140‑1 (MQ=255)                                                  aTTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCgaga                                                                           <  1:312124/140‑1 (MQ=255)                                                  aTTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCgaga                                                                           <  1:795173/140‑1 (MQ=255)                                                  aTTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCAGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCgaga                                                                           <  2:494115/140‑1 (MQ=255)                                                           ttAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGc                                                                  >  2:255931/1‑140 (MQ=255)                                                                     tGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGt                                                        >  2:462101/1‑140 (MQ=255)                                                                     tGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGt                                                        >  2:874752/1‑140 (MQ=255)                                                                      ggCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTc                                                       <  2:267221/140‑1 (MQ=255)                                                                        cAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCa                                                     >  1:915621/1‑140 (MQ=255)                                                                              ttGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGa                                               <  1:874752/140‑1 (MQ=255)                                                                               tGATGTCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGat                                              <  1:489816/140‑1 (MQ=255)                                                                                     ccAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGc                                         <  1:544227/139‑1 (MQ=255)                                                                                     ccAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGc                                         >  2:544227/1‑139 (MQ=255)                                                                                              gAAGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGAtt                               >  1:808126/1‑140 (MQ=255)                                                                                               aaGCAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTc                              >  2:153898/1‑140 (MQ=255)                                                                                                  cAGAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTg                           <  1:81308/140‑1 (MQ=255)                                                                                                    gAAAGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCg                         >  1:439986/1‑140 (MQ=255)                                                                                                     aaaGCATAAAACTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCgg                        <  1:802520/140‑1 (MQ=255)                                                                                                               aCTACCCATATCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAAcagca              >  2:547858/1‑140 (MQ=255)                                                                                                                        atCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGaa     >  1:324302/1‑140 (MQ=255)                                                                                                                        atCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGaa     <  2:808126/140‑1 (MQ=255)                                                                                                                        atCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGaa     <  2:607085/140‑1 (MQ=255)                                                                                                                        atCAGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGaa     <  1:547858/140‑1 (MQ=255)                                                                                                                           aGGCATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAgcg  <  1:153898/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                         AAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCG  >  NC_000913/2471719‑2471980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑