Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,624,311	T→C	V423A (GTG→GCG)	ppk →	polyphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,624,311	0	T	C	100.0%	191.3 / NA	54	V423A (GTG→GCG)	ppk	polyphosphate kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/26);  total (28/26)

GAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTAT  >  NC_000913/2624174‑2624447                                                                                                                                          |                                                                                                                                         gAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGgcgcgc                                                                                                                                        <  2:530169/140‑1 (MQ=255)   aaGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCAc                                                                                                                                      <  1:613995/140‑1 (MQ=255)                catcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTg                                                                                                                         >  1:361708/1‑140 (MQ=255)                  tcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTgcg                                                                                                                       >  1:485645/1‑140 (MQ=255)                      cTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCgg                                                                                                                   >  1:101598/1‑140 (MQ=255)                      cTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCgg                                                                                                                   <  2:444423/140‑1 (MQ=255)                       tCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCggg                                                                                                                  <  2:101598/140‑1 (MQ=255)                        cGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTTACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCgggg                                                                                                                 >  2:977708/1‑140 (MQ=255)                        cGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCgggg                                                                                                                 >  2:74354/1‑140 (MQ=255)                          atgatCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCt                                                                                                               >  2:1042300/1‑140 (MQ=255)                          atgatCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCt                                                                                                               >  1:528639/1‑140 (MQ=255)                                     cgcACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACg                                                                                                    >  2:954354/1‑140 (MQ=255)                                     cgcACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACg                                                                                                    >  2:729730/1‑140 (MQ=255)                                     cgcACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACg                                                                                                    >  2:964956/1‑140 (MQ=255)                                       cacaTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGcc                                                                                                  >  1:1073617/1‑140 (MQ=255)                                         caTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCaa                                                                                                >  1:335211/1‑140 (MQ=255)                                           tAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAAc                                                                                              <  2:314441/140‑1 (MQ=255)                                                 tAAGAAAGTCACCGTGGTGGTTGAGTTACAGTCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACg                                                                                                    >  1:121242/1‑128 (MQ=255)                                                 tAAGAAAGTCACCGTGGTGGTTGAGTTACAGTCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACg                                                                                                    <  2:121242/128‑1 (MQ=255)                                                 tAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTTACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTcc                                                                                        >  1:926078/1‑140 (MQ=255)                                                     aaaGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTTACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGAt                                                                                    <  2:926078/140‑1 (MQ=255)                                                      aaGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGAtt                                                                                   >  2:406557/1‑140 (MQ=255)                                                        gTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTc                                                                                 >  2:968088/1‑140 (MQ=255)                                                           aCCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACg                                                                              >  1:864511/1‑140 (MQ=255)                                                            ccGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGt                                                                             <  1:1042300/140‑1 (MQ=255)                                                               tggtggTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTaaa                                                                          >  1:689586/1‑140 (MQ=255)                                                                       gAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACgg                                                                  >  2:438040/1‑140 (MQ=255)                                                                          ttACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGa                                                               >  1:508438/1‑140 (MQ=255)                                                                              aGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAgtg                                                           >  2:364627/1‑140 (MQ=255)                                                                               ggCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTg                                                                <  1:830783/134‑1 (MQ=255)                                                                               ggCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTg                                                                >  2:830783/1‑134 (MQ=255)                                                                                gcgcGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAgtggt                                                         <  2:770558/140‑1 (MQ=255)                                                                                   cgTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCg                                                      >  2:1065340/1‑140 (MQ=255)                                                                                    gTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGt                                                     <  2:528639/140‑1 (MQ=255)                                                                                     tttCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGtt                                                    <  1:438040/140‑1 (MQ=255)                                                                                          acgaAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGca                                               <  1:968088/140‑1 (MQ=255)                                                                                          acgaAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGca                                               <  2:891695/140‑1 (MQ=255)                                                                                          acgaAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGca                                               <  2:335211/140‑1 (MQ=255)                                                                                          acgaAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGca                                               <  1:364627/140‑1 (MQ=255)                                                                                          acgaAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCAAGTAAAGAAAACGGTGAAGTGGTGCGTTATGca                                               <  2:1073617/140‑1 (MQ=255)                                                                                                            aCTGGGCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAAc                             >  1:511256/1‑140 (MQ=255)                                                                                                                ggCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTa                         >  1:524947/1‑140 (MQ=255)                                                                                                                 gCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCggg                                <  2:259179/132‑1 (MQ=255)                                                                                                                 gCGAAGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCggg                                >  1:259179/1‑132 (MQ=255)                                                                                                                     aGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAATGaa                    >  1:823347/1‑140 (MQ=255)                                                                                                                     aGCGCCTGACCGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGaa                    >  1:953972/1‑140 (MQ=255)                                                                                                                              ccGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACcgcg           <  2:524947/140‑1 (MQ=255)                                                                                                                              ccGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACcgcg           <  1:1065340/140‑1 (MQ=255)                                                                                                                              ccGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACcgcg           <  1:1006712/140‑1 (MQ=255)                                                                                                                              ccGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACcgcg           <  1:977708/140‑1 (MQ=255)                                                                                                                              ccGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACcgcg           <  2:864511/140‑1 (MQ=255)                                                                                                                              ccGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACcgcg           <  2:953972/140‑1 (MQ=255)                                                                                                                              ccGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACcgcg           <  1:410487/140‑1 (MQ=255)                                                                                                                              ccGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACcgcg           <  1:406557/140‑1 (MQ=255)                                                                                                                              ccGAAGCAGGCGCGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGCAGAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACcgcg           <  1:729730/140‑1 (MQ=255)                                                                                                                                       gcgcgcACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTtat  <  2:823347/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCCGGGGCTGAAAATTCACGCCAAACTGTTCCTGATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTAT  >  NC_000913/2624174‑2624447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑