Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,840,452	A→G	H310R (CAC→CGC)	ascF →	beta‑glucoside specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,840,452	0	A	G	100.0%	245.4 / NA	68	H310R (CAC→CGC)	ascF	beta‑glucoside specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/35);  total (33/35)

CCTGCTGATTGGCCCGATTGGTATCTGGATCGGTAGCGCCATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCACCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAAC  >  NC_000913/2840315‑2840585                                                                                                                                          |                                                                                                                                      ccTGCTGATTGGCCCGATTGGTATCTGGATCGGTAGCGCCATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGcc                                                                                                                                     >  1:513313/1‑140 (MQ=255)                  ttGGTATCTGGATCGGTAGCGCCATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAAcc                                                                                                                    >  1:191767/1‑140 (MQ=255)                        tCTGGATCGGTAGCGCCATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCAtt                                                                                                              <  1:670510/140‑1 (MQ=255)                        tCTGGATCGGTAGCGCCATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCAtt                                                                                                              <  1:581935/140‑1 (MQ=255)                             aTCGGTAGCGCCATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGAc                                                                                                         >  2:210233/1‑140 (MQ=255)                             aTCGGTAGCGCCATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGAc                                                                                                         >  2:918643/1‑140 (MQ=255)                               cGGTAGCGCCATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCa                                                                                                       >  2:556657/1‑140 (MQ=255)                                  tAGCGCCATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTg                                                                                                    >  2:952798/1‑140 (MQ=255)                                    gcgcCATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGcc                                                                                                  >  1:1053663/1‑140 (MQ=255)                                       ccATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGaa                                                                                               >  1:281342/1‑140 (MQ=255)                                       ccATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGaa                                                                                               >  1:233948/1‑140 (MQ=255)                                         aTTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAAc                                                                                             <  1:556657/140‑1 (MQ=255)                                           ttCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCg                                                                                           <  2:1059862/140‑1 (MQ=255)                                            tCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCgg                                                                                          <  2:96093/140‑1 (MQ=255)                                              ggCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCa                                                                                        <  1:952798/140‑1 (MQ=255)                                               gcgcTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCg                                                                                           >  2:131675/1‑136 (MQ=255)                                               gcgcTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCg                                                                                           <  1:131675/136‑1 (MQ=255)                                                  cTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAaaga                                                                                    <  2:249390/140‑1 (MQ=255)                                                    ggTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAaagaag                                                                                  <  1:590896/140‑1 (MQ=255)                                                      tttACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAggg                                                                                <  1:561781/140‑1 (MQ=255)                                                             cATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCa                                                                         >  2:99320/1‑140 (MQ=255)                                                              aTTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCAt                                                                        <  2:191767/140‑1 (MQ=255)                                                               ttCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCa                                                                         <  2:507468/138‑1 (MQ=255)                                                               ttCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCa                                                                         >  1:507468/1‑138 (MQ=255)                                                               ttCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATg                                                                       >  2:451796/1‑140 (MQ=255)                                                               ttCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATg                                                                       >  1:773307/1‑140 (MQ=255)                                                               ttCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATg                                                                       >  2:321698/1‑140 (MQ=255)                                                               ttCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATg                                                                       >  1:825226/1‑140 (MQ=255)                                                                tCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGc                                                                      <  2:1053663/140‑1 (MQ=255)                                                                tCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGc                                                                      >  2:15993/1‑140 (MQ=255)                                                                  aTGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCg                                                                    <  1:451796/140‑1 (MQ=255)                                                                    ggTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTc                                                                  >  1:313510/1‑140 (MQ=255)                                                                          cTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGAt                                                            <  1:99320/140‑1 (MQ=255)                                                                          cTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGAt                                                            <  2:825226/140‑1 (MQ=255)                                                                          cTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGAt                                                            <  2:513313/140‑1 (MQ=255)                                                                              gctggctTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGgc                                                        >  1:991594/1‑140 (MQ=255)                                                                                  gctTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTa                                                    >  1:155781/1‑140 (MQ=255)                                                                                  gctTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTa                                                    >  2:626518/1‑140 (MQ=255)                                                                                    tttCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAAc                                                  <  2:1025067/140‑1 (MQ=255)                                                                                       cAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTg                                               >  1:706727/1‑140 (MQ=255)                                                                                            gCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGAt                                                            >  1:846289/1‑122 (MQ=255)                                                                                            gCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGAt                                                            <  2:846289/122‑1 (MQ=255)                                                                                            gCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCt                                          >  1:298423/1‑140 (MQ=255)                                                                                               aTTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTggg                                       <  1:408441/140‑1 (MQ=255)                                                                                               aTTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTggg                                       <  2:233948/140‑1 (MQ=255)                                                                                               aTTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTggg                                       <  2:281342/140‑1 (MQ=255)                                                                                               aTTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTggg                                       <  1:918643/140‑1 (MQ=255)                                                                                                   tGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGt                                   <  2:125233/140‑1 (MQ=255)                                                                                                   tGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGt                                   <  2:298423/140‑1 (MQ=255)                                                                                                    gggCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGtt                                  <  2:706727/140‑1 (MQ=255)                                                                                                     ggCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTtc                                 <  1:291259/140‑1 (MQ=255)                                                                                                     ggCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTtc                                 <  1:767927/140‑1 (MQ=255)                                                                                                      gcgcgcTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTtca                                <  2:991594/140‑1 (MQ=255)                                                                                                       cgcgcTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTtc                                 <  2:248714/138‑1 (MQ=255)                                                                                                       cgcgcTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTtc                                 >  1:248714/1‑138 (MQ=255)                                                                                                        gcgcTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTtcatc                              >  2:972821/1‑140 (MQ=255)                                                                                                         cgcTGTGGCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTtcatca                             >  2:434381/1‑140 (MQ=255)                                                                                                               ggCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCa                       <  2:155781/140‑1 (MQ=255)                                                                                                               ggCCTCTGCTGGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCa                       <  1:972821/140‑1 (MQ=255)                                                                                                                      gctgGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCa                       <  2:1044693/133‑1 (MQ=255)                                                                                                                      gctgGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCa                       >  1:1044693/1‑133 (MQ=255)                                                                                                                      gctgGTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGt                >  2:1055719/1‑140 (MQ=255)                                                                                                                          gTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTtcatca                             >  1:911137/1‑123 (MQ=255)                                                                                                                          gTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTtcatca                             <  2:911137/123‑1 (MQ=255)                                                                                                                          gTAATGACCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGaa            >  2:394267/1‑140 (MQ=255)                                                                                                                                aCCGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGaa      <  1:1055719/140‑1 (MQ=255)                                                                                                                                 ccGGGATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGaaa     >  1:789583/1‑140 (MQ=255)                                                                                                                                    ggATGCGCCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAAc  >  1:797690/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      CCTGCTGATTGGCCCGATTGGTATCTGGATCGGTAGCGCCATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAGTCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCACCGCGTCTTTACGCCAACCATCATTCAGACCATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAAC  >  NC_000913/2840315‑2840585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑