Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,148,923	T→C	S343P (TCT→CCT)	gpr →	L‑glyceraldehyde 3‑phosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,148,923	0	T	C	100.0%	142.6 / NA	42	S343P (TCT→CCT)	gpr	L‑glyceraldehyde 3‑phosphate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/22);  total (20/22)

CGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACC  >  NC_000913/3148786‑3149059                                                                                                                                          |                                                                                                                                         cGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCt                                                                                                                                        >  2:725514/1‑140 (MQ=255)             gTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAAt                                                                                                                              <  2:52760/138‑1 (MQ=255)             gTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAAt                                                                                                                              >  1:52760/1‑138 (MQ=255)             gTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGa                                                                                                                            <  2:650128/140‑1 (MQ=255)              tGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGAc                                                                                                                           >  2:878258/1‑140 (MQ=255)              tGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGAc                                                                                                                           <  2:109094/140‑1 (MQ=255)               gCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGAcc                                                                                                                          <  2:842940/140‑1 (MQ=255)               gCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGAcc                                                                                                                          <  2:333051/140‑1 (MQ=255)                        gcgGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTtaataa                                                                                                                 >  2:332399/1‑140 (MQ=255)                         cgGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAAc                                                                                                                >  2:3406/1‑140 (MQ=255)                            aGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACggg                                                                                                             <  2:212866/140‑1 (MQ=255)                                           aCGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGcc                                                                                              >  1:1012314/1‑140 (MQ=255)                                                cAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAat                                                                                         <  1:58451/140‑1 (MQ=255)                                                        gAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAatcagatcag                                                                                 >  2:507317/1‑140 (MQ=255)                                                                   cATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACg                                                                      >  1:1044898/1‑140 (MQ=255)                                                                       tAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGagtc                                                                  >  1:216826/1‑140 (MQ=255)                                                                              aaGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGt                                                           <  2:1044898/140‑1 (MQ=255)                                                                                    cTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGagtcag                                                                >  1:259495/1‑129 (MQ=255)                                                                                    cTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGagtcag                                                                <  2:259495/129‑1 (MQ=255)                                                                                           aGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCAt                                              <  1:725514/140‑1 (MQ=255)                                                                                           aGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCAt                                              <  1:906045/140‑1 (MQ=255)                                                                                             aTTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATca                                            <  2:945523/140‑1 (MQ=255)                                                                                              ttGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATcac                                           <  2:216826/140‑1 (MQ=255)                                                                                                 aTCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCAt                                              >  1:66135/1‑134 (MQ=255)                                                                                                 aTCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCAt                                              <  2:66135/134‑1 (MQ=255)                                                                                                    aGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTGAAGATAGCCCATCACAAATGc                                     >  2:871695/1‑140 (MQ=255)                                                                                                       atatCGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGa                                  >  2:410565/1‑140 (MQ=255)                                                                                                           cGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAAt                                       >  1:686574/1‑131 (MQ=255)                                                                                                           cGCCGATGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAAt                                       <  2:686574/131‑1 (MQ=255)                                                                                                                aTGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACaaa                         >  1:471418/1‑140 (MQ=255)                                                                                                                aTGGCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACaaa                         >  2:118442/1‑140 (MQ=255)                                                                                                                   gCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGt                      <  2:372560/140‑1 (MQ=255)                                                                                                                   gCGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGt                      <  2:789395/140‑1 (MQ=255)                                                                                                                    cGAGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTc                     >  1:558984/1‑140 (MQ=255)                                                                                                                      aGCTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAg                   <  1:507317/140‑1 (MQ=255)                                                                                                                        cTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGAt                 <  1:432554/140‑1 (MQ=255)                                                                                                                        cTGAATCTGTGGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGAt                 >  1:348016/1‑140 (MQ=255)                                                                                                                               tgtgGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAAt          <  1:508063/140‑1 (MQ=255)                                                                                                                                 tgGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGa        <  1:410565/140‑1 (MQ=255)                                                                                                                                 tgGCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGa        >  1:15235/1‑140 (MQ=255)                                                                                                                                  ggCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATa            >  2:90847/1‑135 (MQ=255)                                                                                                                                  ggCAGGCGCCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATa            <  1:90847/135‑1 (MQ=255)                                                                                                                                       gcgcCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTAcc  >  1:566970/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         CGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACC  >  NC_000913/3148786‑3149059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑