Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,163,735	T→C	S747G (AGC→GGC)	parC ←	DNA topoisomerase IV subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,163,735	0	T	C	100.0%	113.6 / NA	33	S747G (AGC→GGC)	parC	DNA topoisomerase IV subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/15);  total (18/15)

AACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGTTTCCCAA  >  NC_000913/3163598‑3163869                                                                                                                                          |                                                                                                                                       aacGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACcgccgc                                                                                                                                      >  2:183013/1‑140 (MQ=255)     ttttCAAGGAATTTTTTATCGTTTCGGGGGGTTGAATACGCTGCCGGATGCCGACTAAACTTTTCACCCGGCGTTCCTTGCAGGGGGGAGGAAACGGCCCCCCCCCCGGCATATTCCTCTCCGCTATCACCGCCGCTGGc                                                                                                                                  <  2:876170/139‑1 (MQ=255)      tttCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCa                                                                                                                                 >  2:498412/1‑140 (MQ=255)         cAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACgg                                                                                                                              >  2:162218/1‑140 (MQ=255)                    ttATCTTTCCGGGGGGTTGAATCCGCTGCGGGAGGACGACTTACCTTTTCACCCGGCTTTCTTTGCAGGGGGGAGAAAACGGCCCCCCCCCCGGCATATTCCTCTCCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTc                                                                                                                   <  2:123791/140‑1 (MQ=255)                             ccGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTTGCACGGCGAGGAGAGTCGATCTCAac                                                                                                          <  1:183013/140‑1 (MQ=255)                               gggggTTAAATCCGCGGCGGGATGACGATTTAACTTTTCACCGGGGTTTCCTGGAAGGGGGGAGAAACCGGCCCCCCCCCGGGAATTTTCCTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAacac                                                                                                        <  2:480750/140‑1 (MQ=255)                                     tGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACAcgatcga                                                                                                  >  1:480750/1‑140 (MQ=255)                                     tGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACAcgatcga                                                                                                  >  2:78907/1‑140 (MQ=255)                                             cTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGc                                                                                          >  2:273248/1‑140 (MQ=255)                                                       acgacTTATCTTTTCACCCGGCTTTCCTGGCAGGGGGGAGGAAACAGCCCCCCCCCCGGCATATTCCTCTCCGCTACCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCa                                                                                <  2:134308/140‑1 (MQ=255)                                                                   ttCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACcgc                                                                    >  2:358426/1‑140 (MQ=255)                                                                   ttCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACcgc                                                                    >  1:92176/1‑140 (MQ=255)                                                                      aTCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCgcggc                                                                 >  2:91608/1‑140 (MQ=255)                                                                            cGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCAc                                                           >  2:171044/1‑140 (MQ=255)                                                                                  ttGCAGGGGGGAGAAAACGCCCCCCCCCCCGGCATTTTACTTTTCTCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGc                                                     <  2:92176/140‑1 (MQ=255)                                                                                   tGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGcc                                                    >  1:631597/1‑140 (MQ=255)                                                                                                      cgcCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAActct                                 >  1:725633/1‑140 (MQ=255)                                                                                                      cgcCCTCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAActct                                 >  2:179575/1‑140 (MQ=255)                                                                                                           tCCCCGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCgg                            >  1:241358/1‑140 (MQ=255)                                                                                                            ccccGGCATATTCCTCTCCCCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCggg                           <  2:941110/140‑1 (MQ=255)                                                                                                             cccGGCATATTACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGgc                          <  2:631597/140‑1 (MQ=255)                                                                                                              cgggCATATTCCTCTCCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGgcg                         <  2:914967/138‑1 (MQ=255)                                                                                                                       tACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAAt                >  1:714942/1‑140 (MQ=255)                                                                                                                       tACTCTTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAAt                >  1:25634/1‑140 (MQ=255)                                                                                                                           ctTCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTg            <  1:498412/140‑1 (MQ=255)                                                                                                                            ttCGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGc           >  1:150409/1‑140 (MQ=255)                                                                                                                             ccGCTATCCCCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCg          <  2:223783/139‑1 (MQ=255)                                                                                                                              cGCTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGt         >  1:34670/1‑140 (MQ=255)                                                                                                                                cTATCCCCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGttt       <  1:740415/140‑1 (MQ=255)                                                                                                                                cTATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGttt       <  1:179575/140‑1 (MQ=255)                                                                                                                                 tATCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGTTTc      >  2:697626/1‑140 (MQ=255)                                                                                                                                   tCACCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGTTTccc    <  1:78907/140‑1 (MQ=255)                                                                                                                                     cccgccgCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGTTTCCCaa  <  1:162218/139‑1 (MQ=255)                                                                                                                                     aCCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGTTTCCCaa  <  2:34670/140‑1 (MQ=255)                                                                                                                                     aCCGCCGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGTTTCCCaa  <  2:714942/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       AACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCAGTGACTTTCTGTAACTCTTCCGGGCGCAGTTTAATTTTGCGTTTCCCAA  >  NC_000913/3163598‑3163869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑