Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,188,182	A→G	pseudogene (751/2434 nt)	yqiG →	putative outer membrane usher protein YqiG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,188,182	0	A	G	100.0%	302.5 / NA	83	pseudogene (751/2434 nt)	yqiG	putative outer membrane usher protein YqiG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/44);  total (39/44)

TCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTACCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGTTCCTCCAGGCCCATTTGCTATTACCAATTT  >  NC_000913/3188047‑3188316                                                                                                                                        |                                                                                                                                       tCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGc                                                                                                                                    <  2:1029692/140‑1 (MQ=255)      gTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGAc                                                                                                                               >  1:645094/1‑140 (MQ=255)      gTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGAc                                                                                                                               >  1:26489/1‑140 (MQ=255)           tatttGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACg                                                                                                                          <  2:930461/140‑1 (MQ=255)                 tttCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTa                                                                                                                    >  1:569546/1‑140 (MQ=255)                   tCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACg                                                                                                                  >  1:649568/1‑140 (MQ=255)                   tCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACg                                                                                                                  >  1:2514/1‑140 (MQ=255)                        cAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCg                                                                                                             <  1:892222/140‑1 (MQ=255)                        cAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCg                                                                                                             <  2:247263/140‑1 (MQ=255)                         aaTCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGc                                                                                                            <  1:433313/140‑1 (MQ=255)                              cAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATc                                                                                                       >  2:869756/1‑140 (MQ=255)                                gCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCAc                                                                                                     <  2:645094/140‑1 (MQ=255)                                 cAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACg                                                                                                    <  1:518378/140‑1 (MQ=255)                                     gaatgCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGgtg                                                                                                >  2:99861/1‑140 (MQ=255)                                      aatgCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCGGACCTACGTGGTTACGCACCGCAAATCACGGgtgt                                                                                               >  2:748707/1‑140 (MQ=255)                                          cAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGca                                                                                           <  1:99861/140‑1 (MQ=255)                                           aaaaCTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGcac                                                                                          >  1:730953/1‑140 (MQ=255)                                            aaaCTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGcaca                                                                                         <  2:608283/140‑1 (MQ=255)                                                 aactaTAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGa                                                                                    <  1:449744/140‑1 (MQ=255)                                                      taGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCa                                                                               <  2:719339/140‑1 (MQ=255)                                                       aGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCaa                                                                              >  2:1020970/1‑140 (MQ=255)                                                       aGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCaa                                                                              <  1:869756/140‑1 (MQ=255)                                                        ggTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCaaa                                                                             >  2:407883/1‑140 (MQ=255)                                                           cAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGt                                                                          <  2:524021/140‑1 (MQ=255)                                                           cAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGt                                                                          <  2:649568/140‑1 (MQ=255)                                                           cAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGt                                                                          <  1:880738/140‑1 (MQ=255)                                                           cAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGt                                                                          <  1:151246/140‑1 (MQ=255)                                                            aaTACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTc                                                                         >  1:178113/1‑140 (MQ=255)                                                             aTACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCa                                                                        <  1:1020970/140‑1 (MQ=255)                                                             aTACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCa                                                                        <  1:407883/140‑1 (MQ=255)                                                             aTACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCa                                                                        <  2:1329/140‑1 (MQ=255)                                                              tACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCAc                                                                       >  2:375520/1‑140 (MQ=255)                                                              tACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCAc                                                                       <  1:375520/140‑1 (MQ=255)                                                                cGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTg                                                                     <  1:708256/140‑1 (MQ=255)                                                                   tACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACGAACGAATGCAAAGGTCACTGtct                                                                  <  1:435764/140‑1 (MQ=255)                                                                   tACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTg                                                                     >  1:237647/1‑137 (MQ=255)                                                                   tACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTg                                                                     <  2:237647/137‑1 (MQ=255)                                                                   tACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGtct                                                                  >  2:344376/1‑140 (MQ=255)                                                                      tGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGc                                                                                <  2:395029/123‑1 (MQ=255)                                                                      tGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGc                                                                                >  1:395029/1‑123 (MQ=255)                                                                      tGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTcac                                                               >  2:791847/1‑140 (MQ=255)                                                                      tGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTcac                                                               >  2:709624/1‑140 (MQ=255)                                                                        aaTCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTca                                                                >  1:505053/1‑137 (MQ=255)                                                                        aaTCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTca                                                                <  2:505053/137‑1 (MQ=255)                                                                        aaTCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTca                                                                >  2:778828/1‑137 (MQ=255)                                                                        aaTCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTca                                                                <  1:778828/137‑1 (MQ=255)                                                                        aaTCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAg                                                             >  2:918375/1‑140 (MQ=255)                                                                        aaTCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAg                                                             >  1:443322/1‑140 (MQ=255)                                                                          tCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGaa                                                           <  1:344376/140‑1 (MQ=255)                                                                             tctATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTca                                                                >  2:412615/1‑132 (MQ=255)                                                                             tctATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTca                                                                <  1:412615/132‑1 (MQ=255)                                                                             tctATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGaacaa                                                        <  2:569546/140‑1 (MQ=255)                                                                             tctATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGaacaa                                                        >  1:422190/1‑140 (MQ=255)                                                                             tctATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGaacaa                                                        <  2:2514/140‑1 (MQ=255)                                                                             tctATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGaac                                                          <  2:288555/138‑1 (MQ=255)                                                                             tctATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGaac                                                          >  1:288555/1‑138 (MQ=255)                                                                             tctATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGaa                                                           >  1:220052/1‑137 (MQ=255)                                                                             tctATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGaa                                                           <  2:220052/137‑1 (MQ=255)                                                                              ctATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGaacaac                                                       <  1:709624/140‑1 (MQ=255)                                                                               tATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAAcc                                                      >  1:861566/1‑140 (MQ=255)                                                                               tATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAAcc                                                      >  2:787290/1‑140 (MQ=255)                                                                               tATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAAcc                                                      >  1:198733/1‑140 (MQ=255)                                                                                      gACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGtattat                                               >  2:39639/1‑140 (MQ=255)                                                                                           tttCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGaac                                                          <  1:671913/124‑1 (MQ=255)                                                                                           tttCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGaac                                                          >  2:671913/1‑124 (MQ=255)                                                                                           tttCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATc                                          >  2:233019/1‑140 (MQ=255)                                                                                           tttCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATc                                          >  2:38430/1‑140 (MQ=255)                                                                                           tttCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATc                                          >  1:182084/1‑140 (MQ=255)                                                                                             tCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCaa                                        <  1:918375/140‑1 (MQ=255)                                                                                                  ttttCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGaaaa                                   <  1:233019/140‑1 (MQ=255)                                                                                                     tCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGt                                <  2:730953/140‑1 (MQ=255)                                                                                                     tCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGt                                >  2:741384/1‑140 (MQ=255)                                                                                                       tGGCATTTCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGTtc                              >  1:1076027/1‑140 (MQ=255)                                                                                                              tCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGTTCCTCCAgg                       >  1:195354/1‑140 (MQ=255)                                                                                                              tCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGTTCCTCCAgg                       >  2:75769/1‑140 (MQ=255)                                                                                                              tCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGTTCCTCCAgg                       <  1:39639/140‑1 (MQ=255)                                                                                                              tCGTTGAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGTTCCTCCAgg                       >  1:245321/1‑140 (MQ=255)                                                                                                                   gAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGTTCCTCCAGGCCCAt                  <  1:791847/140‑1 (MQ=255)                                                                                                                   gAAAAGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGTTCCTCCAGGCCCAt                  <  1:809379/140‑1 (MQ=255)                                                                                                                       aGCGATGAGAATATGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGTTCCTCCAGGCCCATTTGc              <  2:26489/140‑1 (MQ=255)                                                                                                                                 atatGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGTTCCTCCAGGCCCATTTGCTATTACCAAt    <  1:787290/140‑1 (MQ=255)                                                                                                                                 atatGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGTTCCTCCAGGCCCATTTGCTATTACCAAt    <  2:939881/140‑1 (MQ=255)                                                                                                                                   atGTTGCCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGTTCCTCCAGGCCCATTTGCTATTACCAAttt  <  1:741384/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAGCGATGAGAATATGTTACCGCCAGACCTACGTGGTTACGCACCGCAAATCACGGGTGTCGCACAAACGAATGCAAAGGTCACTGTCTCACAGAACAACCGTATTATTTATCAAGAAAATGTTCCTCCAGGCCCATTTGCTATTACCAATTT  >  NC_000913/3188047‑3188316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑